Quantum-Dot-Based Immunochromatographic Assay for Total IgE in Human Serum

To rapidly quantify total immunoglobulin E levels in human serum, we developed a novel quantum-dot-based immunochromatographic assay that employs digital recording of fluorescence. It can detect IgE levels of 5–1000 kU/L, with a coefficient of variation ranging from 2.0 to 9.5%. The assay can be pro...

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Detalles Bibliográficos
Autores principales: Berlina, Anna N., Taranova, Nadezhda A., Zherdev, Anatoly V., Sankov, Mikhail N., Andreev, Igor V., Martynov, Alexandr I., Dzantiev, Boris B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813722/
https://www.ncbi.nlm.nih.gov/pubmed/24204841
http://dx.doi.org/10.1371/journal.pone.0077485
Descripción
Sumario:To rapidly quantify total immunoglobulin E levels in human serum, we developed a novel quantum-dot-based immunochromatographic assay that employs digital recording of fluorescence. It can detect IgE levels of 5–1000 kU/L, with a coefficient of variation ranging from 2.0 to 9.5%. The assay can be processed in 10 min. The developed assay was tested on 95 serum samples. The correlation coefficient between the IgE values obtained by the proposed assay and those obtained by a commercial ELISA kit was 0.9884. Our assay thus shows promise as a new diagnostic tool for IgE detection.

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