Quantum-Dot-Based Immunochromatographic Assay for Total IgE in Human Serum

To rapidly quantify total immunoglobulin E levels in human serum, we developed a novel quantum-dot-based immunochromatographic assay that employs digital recording of fluorescence. It can detect IgE levels of 5–1000 kU/L, with a coefficient of variation ranging from 2.0 to 9.5%. The assay can be pro...

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Autores principales: Berlina, Anna N., Taranova, Nadezhda A., Zherdev, Anatoly V., Sankov, Mikhail N., Andreev, Igor V., Martynov, Alexandr I., Dzantiev, Boris B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813722/
https://www.ncbi.nlm.nih.gov/pubmed/24204841
http://dx.doi.org/10.1371/journal.pone.0077485
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author Berlina, Anna N.
Taranova, Nadezhda A.
Zherdev, Anatoly V.
Sankov, Mikhail N.
Andreev, Igor V.
Martynov, Alexandr I.
Dzantiev, Boris B.
author_facet Berlina, Anna N.
Taranova, Nadezhda A.
Zherdev, Anatoly V.
Sankov, Mikhail N.
Andreev, Igor V.
Martynov, Alexandr I.
Dzantiev, Boris B.
author_sort Berlina, Anna N.
collection PubMed
description To rapidly quantify total immunoglobulin E levels in human serum, we developed a novel quantum-dot-based immunochromatographic assay that employs digital recording of fluorescence. It can detect IgE levels of 5–1000 kU/L, with a coefficient of variation ranging from 2.0 to 9.5%. The assay can be processed in 10 min. The developed assay was tested on 95 serum samples. The correlation coefficient between the IgE values obtained by the proposed assay and those obtained by a commercial ELISA kit was 0.9884. Our assay thus shows promise as a new diagnostic tool for IgE detection.
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spelling pubmed-38137222013-11-07 Quantum-Dot-Based Immunochromatographic Assay for Total IgE in Human Serum Berlina, Anna N. Taranova, Nadezhda A. Zherdev, Anatoly V. Sankov, Mikhail N. Andreev, Igor V. Martynov, Alexandr I. Dzantiev, Boris B. PLoS One Research Article To rapidly quantify total immunoglobulin E levels in human serum, we developed a novel quantum-dot-based immunochromatographic assay that employs digital recording of fluorescence. It can detect IgE levels of 5–1000 kU/L, with a coefficient of variation ranging from 2.0 to 9.5%. The assay can be processed in 10 min. The developed assay was tested on 95 serum samples. The correlation coefficient between the IgE values obtained by the proposed assay and those obtained by a commercial ELISA kit was 0.9884. Our assay thus shows promise as a new diagnostic tool for IgE detection. Public Library of Science 2013-10-30 /pmc/articles/PMC3813722/ /pubmed/24204841 http://dx.doi.org/10.1371/journal.pone.0077485 Text en © 2013 Berlina et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Berlina, Anna N.
Taranova, Nadezhda A.
Zherdev, Anatoly V.
Sankov, Mikhail N.
Andreev, Igor V.
Martynov, Alexandr I.
Dzantiev, Boris B.
Quantum-Dot-Based Immunochromatographic Assay for Total IgE in Human Serum
title Quantum-Dot-Based Immunochromatographic Assay for Total IgE in Human Serum
title_full Quantum-Dot-Based Immunochromatographic Assay for Total IgE in Human Serum
title_fullStr Quantum-Dot-Based Immunochromatographic Assay for Total IgE in Human Serum
title_full_unstemmed Quantum-Dot-Based Immunochromatographic Assay for Total IgE in Human Serum
title_short Quantum-Dot-Based Immunochromatographic Assay for Total IgE in Human Serum
title_sort quantum-dot-based immunochromatographic assay for total ige in human serum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813722/
https://www.ncbi.nlm.nih.gov/pubmed/24204841
http://dx.doi.org/10.1371/journal.pone.0077485
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