CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans

The generation of genetic mutants in Caenorhabditis elegans has long relied on the selection of mutations in large-scale screens. Directed mutagenesis of specific loci in the genome would greatly speed up analysis of gene function. Here, we adapt the CRISPR/Cas9 system to generate mutations at speci...

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Autores principales: Waaijers, Selma, Portegijs, Vincent, Kerver, Jana, Lemmens, Bennie B. L. G., Tijsterman, Marcel, van den Heuvel, Sander, Boxem, Mike
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2013
Materias:
Notes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813849/
https://www.ncbi.nlm.nih.gov/pubmed/23979586
http://dx.doi.org/10.1534/genetics.113.156299
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author Waaijers, Selma
Portegijs, Vincent
Kerver, Jana
Lemmens, Bennie B. L. G.
Tijsterman, Marcel
van den Heuvel, Sander
Boxem, Mike
author_facet Waaijers, Selma
Portegijs, Vincent
Kerver, Jana
Lemmens, Bennie B. L. G.
Tijsterman, Marcel
van den Heuvel, Sander
Boxem, Mike
author_sort Waaijers, Selma
collection PubMed
description The generation of genetic mutants in Caenorhabditis elegans has long relied on the selection of mutations in large-scale screens. Directed mutagenesis of specific loci in the genome would greatly speed up analysis of gene function. Here, we adapt the CRISPR/Cas9 system to generate mutations at specific sites in the C. elegans genome.
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spelling pubmed-38138492013-11-01 CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans Waaijers, Selma Portegijs, Vincent Kerver, Jana Lemmens, Bennie B. L. G. Tijsterman, Marcel van den Heuvel, Sander Boxem, Mike Genetics Notes The generation of genetic mutants in Caenorhabditis elegans has long relied on the selection of mutations in large-scale screens. Directed mutagenesis of specific loci in the genome would greatly speed up analysis of gene function. Here, we adapt the CRISPR/Cas9 system to generate mutations at specific sites in the C. elegans genome. Genetics Society of America 2013-11 /pmc/articles/PMC3813849/ /pubmed/23979586 http://dx.doi.org/10.1534/genetics.113.156299 Text en Copyright © 2013 by the Genetics Society of America Available freely online through the author-supported open access option.
spellingShingle Notes
Waaijers, Selma
Portegijs, Vincent
Kerver, Jana
Lemmens, Bennie B. L. G.
Tijsterman, Marcel
van den Heuvel, Sander
Boxem, Mike
CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans
title CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans
title_full CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans
title_fullStr CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans
title_full_unstemmed CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans
title_short CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans
title_sort crispr/cas9-targeted mutagenesis in caenorhabditis elegans
topic Notes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813849/
https://www.ncbi.nlm.nih.gov/pubmed/23979586
http://dx.doi.org/10.1534/genetics.113.156299
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