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CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans

The generation of genetic mutants in Caenorhabditis elegans has long relied on the selection of mutations in large-scale screens. Directed mutagenesis of specific loci in the genome would greatly speed up analysis of gene function. Here, we adapt the CRISPR/Cas9 system to generate mutations at speci...

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Detalles Bibliográficos
Autores principales:	Waaijers, Selma, Portegijs, Vincent, Kerver, Jana, Lemmens, Bennie B. L. G., Tijsterman, Marcel, van den Heuvel, Sander, Boxem, Mike
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Genetics Society of America 2013
Materias:	Notes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813849/ https://www.ncbi.nlm.nih.gov/pubmed/23979586 http://dx.doi.org/10.1534/genetics.113.156299

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