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The DNA damage and the DNA replication checkpoints converge at the MBF transcription factor

In fission yeast cells, Cds1 is the effector kinase of the DNA replication checkpoint. We previously showed that when the DNA replication checkpoint is activated, the repressor Yox1 is phosphorylated and inactivated by Cds1, resulting in activation of MluI-binding factor (MBF)–dependent transcriptio...

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Autores principales: Ivanova, Tsvetomira, Alves-Rodrigues, Isabel, Gómez-Escoda, Blanca, Dutta, Chaitali, DeCaprio, James A., Rhind, Nick, Hidalgo, Elena, Ayté, José
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3814153/
https://www.ncbi.nlm.nih.gov/pubmed/24006488
http://dx.doi.org/10.1091/mbc.E13-05-0257
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author Ivanova, Tsvetomira
Alves-Rodrigues, Isabel
Gómez-Escoda, Blanca
Dutta, Chaitali
DeCaprio, James A.
Rhind, Nick
Hidalgo, Elena
Ayté, José
author_facet Ivanova, Tsvetomira
Alves-Rodrigues, Isabel
Gómez-Escoda, Blanca
Dutta, Chaitali
DeCaprio, James A.
Rhind, Nick
Hidalgo, Elena
Ayté, José
author_sort Ivanova, Tsvetomira
collection PubMed
description In fission yeast cells, Cds1 is the effector kinase of the DNA replication checkpoint. We previously showed that when the DNA replication checkpoint is activated, the repressor Yox1 is phosphorylated and inactivated by Cds1, resulting in activation of MluI-binding factor (MBF)–dependent transcription. This is essential to reinitiate DNA synthesis and for correct G1-to-S transition. Here we show that Cdc10, which is an essential part of the MBF core, is the target of the DNA damage checkpoint. When fission yeast cells are treated with DNA-damaging agents, Chk1 is activated and phosphorylates Cdc10 at its carboxy-terminal domain. This modification is responsible for the repression of MBF-dependent transcription through induced release of MBF from chromatin. This inactivation of MBF is important for survival of cells challenged with DNA-damaging agents. Thus Yox1 and Cdc10 couple normal cell cycle regulation in unperturbed conditions and the DNA replication and DNA damage checkpoints into a single transcriptional complex.
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spelling pubmed-38141532014-01-16 The DNA damage and the DNA replication checkpoints converge at the MBF transcription factor Ivanova, Tsvetomira Alves-Rodrigues, Isabel Gómez-Escoda, Blanca Dutta, Chaitali DeCaprio, James A. Rhind, Nick Hidalgo, Elena Ayté, José Mol Biol Cell Articles In fission yeast cells, Cds1 is the effector kinase of the DNA replication checkpoint. We previously showed that when the DNA replication checkpoint is activated, the repressor Yox1 is phosphorylated and inactivated by Cds1, resulting in activation of MluI-binding factor (MBF)–dependent transcription. This is essential to reinitiate DNA synthesis and for correct G1-to-S transition. Here we show that Cdc10, which is an essential part of the MBF core, is the target of the DNA damage checkpoint. When fission yeast cells are treated with DNA-damaging agents, Chk1 is activated and phosphorylates Cdc10 at its carboxy-terminal domain. This modification is responsible for the repression of MBF-dependent transcription through induced release of MBF from chromatin. This inactivation of MBF is important for survival of cells challenged with DNA-damaging agents. Thus Yox1 and Cdc10 couple normal cell cycle regulation in unperturbed conditions and the DNA replication and DNA damage checkpoints into a single transcriptional complex. The American Society for Cell Biology 2013-11-01 /pmc/articles/PMC3814153/ /pubmed/24006488 http://dx.doi.org/10.1091/mbc.E13-05-0257 Text en © 2013 Ivanova et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Ivanova, Tsvetomira
Alves-Rodrigues, Isabel
Gómez-Escoda, Blanca
Dutta, Chaitali
DeCaprio, James A.
Rhind, Nick
Hidalgo, Elena
Ayté, José
The DNA damage and the DNA replication checkpoints converge at the MBF transcription factor
title The DNA damage and the DNA replication checkpoints converge at the MBF transcription factor
title_full The DNA damage and the DNA replication checkpoints converge at the MBF transcription factor
title_fullStr The DNA damage and the DNA replication checkpoints converge at the MBF transcription factor
title_full_unstemmed The DNA damage and the DNA replication checkpoints converge at the MBF transcription factor
title_short The DNA damage and the DNA replication checkpoints converge at the MBF transcription factor
title_sort dna damage and the dna replication checkpoints converge at the mbf transcription factor
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3814153/
https://www.ncbi.nlm.nih.gov/pubmed/24006488
http://dx.doi.org/10.1091/mbc.E13-05-0257
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