Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice

The type II CRISPR/Cas system from Streptococcus pyogenes and its simplified derivative, the Cas9/single guide RNA (sgRNA) system, have emerged as potent new tools for targeted gene knockout in bacteria, yeast, fruit fly, zebrafish and human cells. Here, we describe adaptations of these systems lead...

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Detalles Bibliográficos
Autores principales: Jiang, Wenzhi, Zhou, Huanbin, Bi, Honghao, Fromm, Michael, Yang, Bing, Weeks, Donald P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3814374/
https://www.ncbi.nlm.nih.gov/pubmed/23999092
http://dx.doi.org/10.1093/nar/gkt780

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3814374/
https://www.ncbi.nlm.nih.gov/pubmed/23999092
http://dx.doi.org/10.1093/nar/gkt780

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