Reversed-phase ion-pair liquid chromatography method for purification of duplex DNA with single base pair resolution

Obtaining quantities of highly pure duplex DNA is a bottleneck in the biophysical analysis of protein–DNA complexes. In traditional DNA purification methods, the individual cognate DNA strands are purified separately before annealing to form DNA duplexes. This approach works well for palindromic seq...

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Detalles Bibliográficos
Autores principales: Wysoczynski, Christina L., Roemer, Sarah C., Dostal, Vishantie, Barkley, Robert M., Churchill, Mair E. A., Malarkey, Christopher S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3814375/
https://www.ncbi.nlm.nih.gov/pubmed/24013567
http://dx.doi.org/10.1093/nar/gkt815

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3814375/
https://www.ncbi.nlm.nih.gov/pubmed/24013567
http://dx.doi.org/10.1093/nar/gkt815

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