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Efficient genome editing in Caenorhabditis elegans by CRISPR-targeted homologous recombination

Cas9 is an RNA-guided double-stranded DNA nuclease that participates in clustered regularly interspaced short palindromic repeats (CRISPR)-mediated adaptive immunity in prokaryotes. CRISPR–Cas9 has recently been used to generate insertion and deletion mutations in Caenorhabditis elegans, but not to...

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Detalles Bibliográficos
Autores principales:	Chen, Changchun, Fenk, Lorenz A., de Bono, Mario
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2013
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3814388/ https://www.ncbi.nlm.nih.gov/pubmed/24013562 http://dx.doi.org/10.1093/nar/gkt805

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