Cargando…
Cytokines and Adhesion Molecules Expression in the Brain in Human Cerebral Malaria
Although the role of systemic proinflammatory cytokines, IL-1β and TNF-α, and their up-regulation of adhesion molecules, ICAM-1, VCAM-1 and E-Selectin, in the pathogenesis of cerebral malaria (CM) is well established, the role of local cytokine release remain unclear. Immunohistochemistry (IHC) was...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2005
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3814706/ https://www.ncbi.nlm.nih.gov/pubmed/16705810 |
_version_ | 1782289299235930112 |
---|---|
author | Armah, Henry Wiredu, Edwin Kwame Dodoo, Alfred Kofi Adjei, Andrew Anthony Tettey, Yao Gyasi, Richard |
author_facet | Armah, Henry Wiredu, Edwin Kwame Dodoo, Alfred Kofi Adjei, Andrew Anthony Tettey, Yao Gyasi, Richard |
author_sort | Armah, Henry |
collection | PubMed |
description | Although the role of systemic proinflammatory cytokines, IL-1β and TNF-α, and their up-regulation of adhesion molecules, ICAM-1, VCAM-1 and E-Selectin, in the pathogenesis of cerebral malaria (CM) is well established, the role of local cytokine release remain unclear. Immunohistochemistry (IHC) was used to compare the expression of ICAM-1, VCAM-1, E-Selectin, IL-1β, TNF-α and TGF- β at light microscopic level in cerebral, cerebellar and brainstem postmortem cryostat sections from 10 CM, 5 severe malarial anemia (SMA), 1 purulent bacterial meningitis (PBM), 2 non-central nervous system infections (NCNSI) and 3 non-infections (NI) deaths in Ghanaian children. Fatal malaria and Salmonella sepsis showed significantly higher vascular expression of all 3 adhesion molecules, with highly significant co-localization with sequestration in the malaria cases. However, there was negligible difference between CM and SMA. TGF-β showed intravascular and perivascular distribution in all cases, but expression was most intense in the PBM case and CM group. TNF-α and IL-1β showed prominent brain parenchymal staining, in addition to intravascular and perivascular staining, in only the PBM case and CM group. The maximal expression of all 6 antigens studied was in the cerebellar sections of the malaria cases. Endothelial activation is a feature of fatal malaria and Salmonella sepsis, with adhesion molecule expression being highly correlated with sequestration. IL-1β and TNF-α are upregulated in only cases with neurodegenerative lesions, whilst TGF-β is present in all cases. Both cytokines and adhesion molecules were maximally upregulated in the cerebellar sections of the malaria cases. |
format | Online Article Text |
id | pubmed-3814706 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-38147062013-11-04 Cytokines and Adhesion Molecules Expression in the Brain in Human Cerebral Malaria Armah, Henry Wiredu, Edwin Kwame Dodoo, Alfred Kofi Adjei, Andrew Anthony Tettey, Yao Gyasi, Richard Int J Environ Res Public Health Article Although the role of systemic proinflammatory cytokines, IL-1β and TNF-α, and their up-regulation of adhesion molecules, ICAM-1, VCAM-1 and E-Selectin, in the pathogenesis of cerebral malaria (CM) is well established, the role of local cytokine release remain unclear. Immunohistochemistry (IHC) was used to compare the expression of ICAM-1, VCAM-1, E-Selectin, IL-1β, TNF-α and TGF- β at light microscopic level in cerebral, cerebellar and brainstem postmortem cryostat sections from 10 CM, 5 severe malarial anemia (SMA), 1 purulent bacterial meningitis (PBM), 2 non-central nervous system infections (NCNSI) and 3 non-infections (NI) deaths in Ghanaian children. Fatal malaria and Salmonella sepsis showed significantly higher vascular expression of all 3 adhesion molecules, with highly significant co-localization with sequestration in the malaria cases. However, there was negligible difference between CM and SMA. TGF-β showed intravascular and perivascular distribution in all cases, but expression was most intense in the PBM case and CM group. TNF-α and IL-1β showed prominent brain parenchymal staining, in addition to intravascular and perivascular staining, in only the PBM case and CM group. The maximal expression of all 6 antigens studied was in the cerebellar sections of the malaria cases. Endothelial activation is a feature of fatal malaria and Salmonella sepsis, with adhesion molecule expression being highly correlated with sequestration. IL-1β and TNF-α are upregulated in only cases with neurodegenerative lesions, whilst TGF-β is present in all cases. Both cytokines and adhesion molecules were maximally upregulated in the cerebellar sections of the malaria cases. Molecular Diversity Preservation International (MDPI) 2005-05 2005-04-30 /pmc/articles/PMC3814706/ /pubmed/16705810 Text en © 2005 MDPI. All rights reserved. |
spellingShingle | Article Armah, Henry Wiredu, Edwin Kwame Dodoo, Alfred Kofi Adjei, Andrew Anthony Tettey, Yao Gyasi, Richard Cytokines and Adhesion Molecules Expression in the Brain in Human Cerebral Malaria |
title | Cytokines and Adhesion Molecules Expression in the Brain in Human Cerebral Malaria |
title_full | Cytokines and Adhesion Molecules Expression in the Brain in Human Cerebral Malaria |
title_fullStr | Cytokines and Adhesion Molecules Expression in the Brain in Human Cerebral Malaria |
title_full_unstemmed | Cytokines and Adhesion Molecules Expression in the Brain in Human Cerebral Malaria |
title_short | Cytokines and Adhesion Molecules Expression in the Brain in Human Cerebral Malaria |
title_sort | cytokines and adhesion molecules expression in the brain in human cerebral malaria |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3814706/ https://www.ncbi.nlm.nih.gov/pubmed/16705810 |
work_keys_str_mv | AT armahhenry cytokinesandadhesionmoleculesexpressioninthebraininhumancerebralmalaria AT wireduedwinkwame cytokinesandadhesionmoleculesexpressioninthebraininhumancerebralmalaria AT dodooalfredkofi cytokinesandadhesionmoleculesexpressioninthebraininhumancerebralmalaria AT adjeiandrewanthony cytokinesandadhesionmoleculesexpressioninthebraininhumancerebralmalaria AT tetteyyao cytokinesandadhesionmoleculesexpressioninthebraininhumancerebralmalaria AT gyasirichard cytokinesandadhesionmoleculesexpressioninthebraininhumancerebralmalaria |