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Honey bee protein atlas at organ-level resolution
Genome sequencing has provided us with gene lists but cannot tell us where and how their encoded products work together to support life. Complex organisms rely on differential expression of subsets of genes/proteins in organs and tissues, and, in concert, evolved to their present state as they funct...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3814894/ https://www.ncbi.nlm.nih.gov/pubmed/23878156 http://dx.doi.org/10.1101/gr.155994.113 |
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author | Chan, Queenie W.T. Chan, Man Yi Logan, Michelle Fang, Yuan Higo, Heather Foster, Leonard J. |
author_facet | Chan, Queenie W.T. Chan, Man Yi Logan, Michelle Fang, Yuan Higo, Heather Foster, Leonard J. |
author_sort | Chan, Queenie W.T. |
collection | PubMed |
description | Genome sequencing has provided us with gene lists but cannot tell us where and how their encoded products work together to support life. Complex organisms rely on differential expression of subsets of genes/proteins in organs and tissues, and, in concert, evolved to their present state as they function together to improve an organism's overall reproductive fitness. Proteomics studies of individual organs help us understand their basic functions, but this reductionist approach misses the larger context of the whole organism. This problem could be circumvented if all the organs in an organism were comprehensively studied by the same methodology and analyzed together. Using honey bees (Apis mellifera L.) as a model system, we report here an initial whole proteome of a complex organism, measuring 29 different organ/tissue types among the three honey bee castes: queen, drone, and worker. The data reveal that, e.g., workers have a heightened capacity to deal with environmental toxins and queens have a far more robust pheromone detection system than their nestmates. The data also suggest that workers altruistically sacrifice not only their own reproductive capacity but also their immune potential in favor of their queen. Finally, organ-level resolution of protein expression offers a systematic insight into how organs may have developed. |
format | Online Article Text |
id | pubmed-3814894 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-38148942014-05-01 Honey bee protein atlas at organ-level resolution Chan, Queenie W.T. Chan, Man Yi Logan, Michelle Fang, Yuan Higo, Heather Foster, Leonard J. Genome Res Resource Genome sequencing has provided us with gene lists but cannot tell us where and how their encoded products work together to support life. Complex organisms rely on differential expression of subsets of genes/proteins in organs and tissues, and, in concert, evolved to their present state as they function together to improve an organism's overall reproductive fitness. Proteomics studies of individual organs help us understand their basic functions, but this reductionist approach misses the larger context of the whole organism. This problem could be circumvented if all the organs in an organism were comprehensively studied by the same methodology and analyzed together. Using honey bees (Apis mellifera L.) as a model system, we report here an initial whole proteome of a complex organism, measuring 29 different organ/tissue types among the three honey bee castes: queen, drone, and worker. The data reveal that, e.g., workers have a heightened capacity to deal with environmental toxins and queens have a far more robust pheromone detection system than their nestmates. The data also suggest that workers altruistically sacrifice not only their own reproductive capacity but also their immune potential in favor of their queen. Finally, organ-level resolution of protein expression offers a systematic insight into how organs may have developed. Cold Spring Harbor Laboratory Press 2013-11 /pmc/articles/PMC3814894/ /pubmed/23878156 http://dx.doi.org/10.1101/gr.155994.113 Text en © 2013 Chan et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/3.0/ This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/. |
spellingShingle | Resource Chan, Queenie W.T. Chan, Man Yi Logan, Michelle Fang, Yuan Higo, Heather Foster, Leonard J. Honey bee protein atlas at organ-level resolution |
title | Honey bee protein atlas at organ-level resolution |
title_full | Honey bee protein atlas at organ-level resolution |
title_fullStr | Honey bee protein atlas at organ-level resolution |
title_full_unstemmed | Honey bee protein atlas at organ-level resolution |
title_short | Honey bee protein atlas at organ-level resolution |
title_sort | honey bee protein atlas at organ-level resolution |
topic | Resource |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3814894/ https://www.ncbi.nlm.nih.gov/pubmed/23878156 http://dx.doi.org/10.1101/gr.155994.113 |
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