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Sperm Nuclear Architecture Is Locally Modified in Presence of a Robertsonian Translocation t(13;17)

In mammals, the non-random organization of the sperm nucleus supports an early function during embryonic development. Altering this organization may interfere with the zygote development and reduce fertility or prolificity. Thus, rare studies on sperm cells from infertile patients described an alter...

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Autores principales: Acloque, Hervé, Bonnet-Garnier, Amélie, Mompart, Florence, Pinton, Alain, Yerle-Bouissou, Martine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815027/
https://www.ncbi.nlm.nih.gov/pubmed/24205066
http://dx.doi.org/10.1371/journal.pone.0078005
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author Acloque, Hervé
Bonnet-Garnier, Amélie
Mompart, Florence
Pinton, Alain
Yerle-Bouissou, Martine
author_facet Acloque, Hervé
Bonnet-Garnier, Amélie
Mompart, Florence
Pinton, Alain
Yerle-Bouissou, Martine
author_sort Acloque, Hervé
collection PubMed
description In mammals, the non-random organization of the sperm nucleus supports an early function during embryonic development. Altering this organization may interfere with the zygote development and reduce fertility or prolificity. Thus, rare studies on sperm cells from infertile patients described an altered nuclear organization that may be a cause or a consequence of their respective pathologies. Thereby, chromosomal rearrangements and aneuploidy can be studied not only for their adverse effects on production of normal/balanced gametes at meiosis but also for their possible impact on sperm nuclear architecture and the epigenetic consequences of altered chromosome positioning. We decided to compare the global architecture of sperm nuclei from boars, either with a normal chromosome composition or with a Robertsonian translocation involving chromosomes 13 and 17. We hypothesized that the fusion between these chromosomes may change their spatial organization and we examined to what extend it could also modify the global sperm nuclear architecture. Analysis of telomeres, centromeres and gonosomes repartition does not support a global nuclear disorganization. But specific analysis of chromosomes 13 and 17 territories highlights an influence of chromosome 17 for the positioning of the fused chromosomes within the nucleus. We also observed a specific clustering of centromeres depending of the chromosome subtypes. Altogether our results showed that chromosome fusion does not significantly alter sperm nucleus architecture but suggest that centromere remodelling after chromosome fusion locally impacts chromosome positioning.
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spelling pubmed-38150272013-11-07 Sperm Nuclear Architecture Is Locally Modified in Presence of a Robertsonian Translocation t(13;17) Acloque, Hervé Bonnet-Garnier, Amélie Mompart, Florence Pinton, Alain Yerle-Bouissou, Martine PLoS One Research Article In mammals, the non-random organization of the sperm nucleus supports an early function during embryonic development. Altering this organization may interfere with the zygote development and reduce fertility or prolificity. Thus, rare studies on sperm cells from infertile patients described an altered nuclear organization that may be a cause or a consequence of their respective pathologies. Thereby, chromosomal rearrangements and aneuploidy can be studied not only for their adverse effects on production of normal/balanced gametes at meiosis but also for their possible impact on sperm nuclear architecture and the epigenetic consequences of altered chromosome positioning. We decided to compare the global architecture of sperm nuclei from boars, either with a normal chromosome composition or with a Robertsonian translocation involving chromosomes 13 and 17. We hypothesized that the fusion between these chromosomes may change their spatial organization and we examined to what extend it could also modify the global sperm nuclear architecture. Analysis of telomeres, centromeres and gonosomes repartition does not support a global nuclear disorganization. But specific analysis of chromosomes 13 and 17 territories highlights an influence of chromosome 17 for the positioning of the fused chromosomes within the nucleus. We also observed a specific clustering of centromeres depending of the chromosome subtypes. Altogether our results showed that chromosome fusion does not significantly alter sperm nucleus architecture but suggest that centromere remodelling after chromosome fusion locally impacts chromosome positioning. Public Library of Science 2013-10-31 /pmc/articles/PMC3815027/ /pubmed/24205066 http://dx.doi.org/10.1371/journal.pone.0078005 Text en © 2013 Acloque et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Acloque, Hervé
Bonnet-Garnier, Amélie
Mompart, Florence
Pinton, Alain
Yerle-Bouissou, Martine
Sperm Nuclear Architecture Is Locally Modified in Presence of a Robertsonian Translocation t(13;17)
title Sperm Nuclear Architecture Is Locally Modified in Presence of a Robertsonian Translocation t(13;17)
title_full Sperm Nuclear Architecture Is Locally Modified in Presence of a Robertsonian Translocation t(13;17)
title_fullStr Sperm Nuclear Architecture Is Locally Modified in Presence of a Robertsonian Translocation t(13;17)
title_full_unstemmed Sperm Nuclear Architecture Is Locally Modified in Presence of a Robertsonian Translocation t(13;17)
title_short Sperm Nuclear Architecture Is Locally Modified in Presence of a Robertsonian Translocation t(13;17)
title_sort sperm nuclear architecture is locally modified in presence of a robertsonian translocation t(13;17)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815027/
https://www.ncbi.nlm.nih.gov/pubmed/24205066
http://dx.doi.org/10.1371/journal.pone.0078005
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