A DNA Sequence Element That Advances Replication Origin Activation Time in Saccharomyces cerevisiae

Eukaryotic origins of DNA replication undergo activation at various times in S-phase, allowing the genome to be duplicated in a temporally staggered fashion. In the budding yeast Saccharomyces cerevisiae, the activation times of individual origins are not intrinsic to those origins but are instead g...

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Autores principales: Pohl, Thomas J., Kolor, Katherine, Fangman, Walton L., Brewer, Bonita J., Raghuraman, M. K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2013
Materias:
Investigations
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815058/
https://www.ncbi.nlm.nih.gov/pubmed/24022751
http://dx.doi.org/10.1534/g3.113.008250
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author Pohl, Thomas J.
Kolor, Katherine
Fangman, Walton L.
Brewer, Bonita J.
Raghuraman, M. K.
author_facet Pohl, Thomas J.
Kolor, Katherine
Fangman, Walton L.
Brewer, Bonita J.
Raghuraman, M. K.
author_sort Pohl, Thomas J.
collection PubMed
description Eukaryotic origins of DNA replication undergo activation at various times in S-phase, allowing the genome to be duplicated in a temporally staggered fashion. In the budding yeast Saccharomyces cerevisiae, the activation times of individual origins are not intrinsic to those origins but are instead governed by surrounding sequences. Currently, there are two examples of DNA sequences that are known to advance origin activation time, centromeres and forkhead transcription factor binding sites. By combining deletion and linker scanning mutational analysis with two-dimensional gel electrophoresis to measure fork direction in the context of a two-origin plasmid, we have identified and characterized a 19- to 23-bp and a larger 584-bp DNA sequence that are capable of advancing origin activation time.
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spelling pubmed-38150582013-11-04 A DNA Sequence Element That Advances Replication Origin Activation Time in Saccharomyces cerevisiae Pohl, Thomas J. Kolor, Katherine Fangman, Walton L. Brewer, Bonita J. Raghuraman, M. K. G3 (Bethesda) Investigations Eukaryotic origins of DNA replication undergo activation at various times in S-phase, allowing the genome to be duplicated in a temporally staggered fashion. In the budding yeast Saccharomyces cerevisiae, the activation times of individual origins are not intrinsic to those origins but are instead governed by surrounding sequences. Currently, there are two examples of DNA sequences that are known to advance origin activation time, centromeres and forkhead transcription factor binding sites. By combining deletion and linker scanning mutational analysis with two-dimensional gel electrophoresis to measure fork direction in the context of a two-origin plasmid, we have identified and characterized a 19- to 23-bp and a larger 584-bp DNA sequence that are capable of advancing origin activation time. Genetics Society of America 2013-11-01 /pmc/articles/PMC3815058/ /pubmed/24022751 http://dx.doi.org/10.1534/g3.113.008250 Text en Copyright © 2013 Pohl et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigations
Pohl, Thomas J.
Kolor, Katherine
Fangman, Walton L.
Brewer, Bonita J.
Raghuraman, M. K.
A DNA Sequence Element That Advances Replication Origin Activation Time in Saccharomyces cerevisiae
title A DNA Sequence Element That Advances Replication Origin Activation Time in Saccharomyces cerevisiae
title_full A DNA Sequence Element That Advances Replication Origin Activation Time in Saccharomyces cerevisiae
title_fullStr A DNA Sequence Element That Advances Replication Origin Activation Time in Saccharomyces cerevisiae
title_full_unstemmed A DNA Sequence Element That Advances Replication Origin Activation Time in Saccharomyces cerevisiae
title_short A DNA Sequence Element That Advances Replication Origin Activation Time in Saccharomyces cerevisiae
title_sort dna sequence element that advances replication origin activation time in saccharomyces cerevisiae
topic Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815058/
https://www.ncbi.nlm.nih.gov/pubmed/24022751
http://dx.doi.org/10.1534/g3.113.008250
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