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Small‐molecule elicitation of microbial secondary metabolites
Microbial natural products continue to be an unparalleled resource for pharmaceutical lead discovery, but the rediscovery rate is high. Bacterial and fungal sequencing studies indicate that the biosynthetic potential of many strains is much greater than that observed by fermentation. Prodding the ex...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Blackwell Publishing Ltd
2011
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815259/ https://www.ncbi.nlm.nih.gov/pubmed/21375710 http://dx.doi.org/10.1111/j.1751-7915.2010.00196.x |
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author | Pettit, Robin K. |
author_facet | Pettit, Robin K. |
author_sort | Pettit, Robin K. |
collection | PubMed |
description | Microbial natural products continue to be an unparalleled resource for pharmaceutical lead discovery, but the rediscovery rate is high. Bacterial and fungal sequencing studies indicate that the biosynthetic potential of many strains is much greater than that observed by fermentation. Prodding the expression of such silent (cryptic) pathways will allow us to maximize the chemical diversity available from microorganisms. Cryptic metabolic pathways can be accessed in the laboratory using molecular or cultivation‐based approaches. A targeted approach related to cultivation‐based methods is the application of small‐molecule elicitors to specifically affect transcription of secondary metabolite gene clusters. With the isolation of the novel secondary metabolites lunalides A and B, oxylipins, cladochromes F and G, nygerone A, chaetoglobosin‐542, ‐540 and ‐510, sphaerolone, dihydrosphaerolone, mutolide and pestalone, and the enhanced production of known secondary metabolites like penicillin and bacitracin, chemical elicitation is proving to be an effective way to augment natural product libraries. |
format | Online Article Text |
id | pubmed-3815259 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-38152592014-02-12 Small‐molecule elicitation of microbial secondary metabolites Pettit, Robin K. Microb Biotechnol Minireviews Microbial natural products continue to be an unparalleled resource for pharmaceutical lead discovery, but the rediscovery rate is high. Bacterial and fungal sequencing studies indicate that the biosynthetic potential of many strains is much greater than that observed by fermentation. Prodding the expression of such silent (cryptic) pathways will allow us to maximize the chemical diversity available from microorganisms. Cryptic metabolic pathways can be accessed in the laboratory using molecular or cultivation‐based approaches. A targeted approach related to cultivation‐based methods is the application of small‐molecule elicitors to specifically affect transcription of secondary metabolite gene clusters. With the isolation of the novel secondary metabolites lunalides A and B, oxylipins, cladochromes F and G, nygerone A, chaetoglobosin‐542, ‐540 and ‐510, sphaerolone, dihydrosphaerolone, mutolide and pestalone, and the enhanced production of known secondary metabolites like penicillin and bacitracin, chemical elicitation is proving to be an effective way to augment natural product libraries. Blackwell Publishing Ltd 2011-07 2011-07-06 /pmc/articles/PMC3815259/ /pubmed/21375710 http://dx.doi.org/10.1111/j.1751-7915.2010.00196.x Text en Copyright © 2010 The Authors. Journal compilation © 2010 Society for Applied Microbiology and Blackwell Publishing Ltd |
spellingShingle | Minireviews Pettit, Robin K. Small‐molecule elicitation of microbial secondary metabolites |
title | Small‐molecule elicitation of microbial secondary metabolites |
title_full | Small‐molecule elicitation of microbial secondary metabolites |
title_fullStr | Small‐molecule elicitation of microbial secondary metabolites |
title_full_unstemmed | Small‐molecule elicitation of microbial secondary metabolites |
title_short | Small‐molecule elicitation of microbial secondary metabolites |
title_sort | small‐molecule elicitation of microbial secondary metabolites |
topic | Minireviews |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815259/ https://www.ncbi.nlm.nih.gov/pubmed/21375710 http://dx.doi.org/10.1111/j.1751-7915.2010.00196.x |
work_keys_str_mv | AT pettitrobink smallmoleculeelicitationofmicrobialsecondarymetabolites |