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Reducing Vibrio load in Artemia nauplii using antimicrobial photodynamic therapy: a promising strategy to reduce antibiotic application in shrimp larviculture

We propose antimicrobial photodynamic therapy (aPDT) as an alternative strategy to reduce the use of antibiotics in shrimp larviculture systems. The growth of a multiple antibiotic resistant Vibrio harveyi strain was effectively controlled by treating the cells with Rose Bengal and photosensitizing...

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Detalles Bibliográficos
Autores principales: Asok, Aparna, Arshad, Esha, Jasmin, C., Somnath Pai, S., Bright Singh, I. S., Mohandas, A., Anas, Abdulaziz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815272/
https://www.ncbi.nlm.nih.gov/pubmed/21951316
http://dx.doi.org/10.1111/j.1751-7915.2011.00297.x
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author Asok, Aparna
Arshad, Esha
Jasmin, C.
Somnath Pai, S.
Bright Singh, I. S.
Mohandas, A.
Anas, Abdulaziz
author_facet Asok, Aparna
Arshad, Esha
Jasmin, C.
Somnath Pai, S.
Bright Singh, I. S.
Mohandas, A.
Anas, Abdulaziz
author_sort Asok, Aparna
collection PubMed
description We propose antimicrobial photodynamic therapy (aPDT) as an alternative strategy to reduce the use of antibiotics in shrimp larviculture systems. The growth of a multiple antibiotic resistant Vibrio harveyi strain was effectively controlled by treating the cells with Rose Bengal and photosensitizing for 30 min using a halogen lamp. This resulted in the death of > 50% of the cells within the first 10 min of exposure and the 50% reduction in the cell wall integrity after 30 min could be attributed to the destruction of outer membrane protein of V. harveyi by reactive oxygen intermediates produced during the photosensitization. Further, mesocosm experiments with V. harveyi and Artemia nauplii demonstrated that in 30 min, the aPDT could kill 78.9% and 91.2% of heterotrophic bacterial and Vibrio population respectively. In conclusion, the study demonstrated that aPDT with its rapid action and as yet unreported resistance development possibilities could be a propitious strategy to reduce the use of antibiotics in shrimp larviculture systems and thereby, avoid their hazardous effects on human health and the ecosystem at large.
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spelling pubmed-38152722014-02-12 Reducing Vibrio load in Artemia nauplii using antimicrobial photodynamic therapy: a promising strategy to reduce antibiotic application in shrimp larviculture Asok, Aparna Arshad, Esha Jasmin, C. Somnath Pai, S. Bright Singh, I. S. Mohandas, A. Anas, Abdulaziz Microb Biotechnol Research Articles We propose antimicrobial photodynamic therapy (aPDT) as an alternative strategy to reduce the use of antibiotics in shrimp larviculture systems. The growth of a multiple antibiotic resistant Vibrio harveyi strain was effectively controlled by treating the cells with Rose Bengal and photosensitizing for 30 min using a halogen lamp. This resulted in the death of > 50% of the cells within the first 10 min of exposure and the 50% reduction in the cell wall integrity after 30 min could be attributed to the destruction of outer membrane protein of V. harveyi by reactive oxygen intermediates produced during the photosensitization. Further, mesocosm experiments with V. harveyi and Artemia nauplii demonstrated that in 30 min, the aPDT could kill 78.9% and 91.2% of heterotrophic bacterial and Vibrio population respectively. In conclusion, the study demonstrated that aPDT with its rapid action and as yet unreported resistance development possibilities could be a propitious strategy to reduce the use of antibiotics in shrimp larviculture systems and thereby, avoid their hazardous effects on human health and the ecosystem at large. Blackwell Publishing Ltd 2012-01 2011-12-14 /pmc/articles/PMC3815272/ /pubmed/21951316 http://dx.doi.org/10.1111/j.1751-7915.2011.00297.x Text en Copyright © 2011 The Authors. Microbial Biotechnology © 2011 Society for Applied Microbiology and Blackwell Publishing Ltd
spellingShingle Research Articles
Asok, Aparna
Arshad, Esha
Jasmin, C.
Somnath Pai, S.
Bright Singh, I. S.
Mohandas, A.
Anas, Abdulaziz
Reducing Vibrio load in Artemia nauplii using antimicrobial photodynamic therapy: a promising strategy to reduce antibiotic application in shrimp larviculture
title Reducing Vibrio load in Artemia nauplii using antimicrobial photodynamic therapy: a promising strategy to reduce antibiotic application in shrimp larviculture
title_full Reducing Vibrio load in Artemia nauplii using antimicrobial photodynamic therapy: a promising strategy to reduce antibiotic application in shrimp larviculture
title_fullStr Reducing Vibrio load in Artemia nauplii using antimicrobial photodynamic therapy: a promising strategy to reduce antibiotic application in shrimp larviculture
title_full_unstemmed Reducing Vibrio load in Artemia nauplii using antimicrobial photodynamic therapy: a promising strategy to reduce antibiotic application in shrimp larviculture
title_short Reducing Vibrio load in Artemia nauplii using antimicrobial photodynamic therapy: a promising strategy to reduce antibiotic application in shrimp larviculture
title_sort reducing vibrio load in artemia nauplii using antimicrobial photodynamic therapy: a promising strategy to reduce antibiotic application in shrimp larviculture
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815272/
https://www.ncbi.nlm.nih.gov/pubmed/21951316
http://dx.doi.org/10.1111/j.1751-7915.2011.00297.x
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