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Walking the tightrope of bioavailability: growth dynamics of PAH degraders on vapour‐phase PAH
Microbial contaminant degradation may either result in the utilization of the compound for growth or act as a protective mechanism against its toxicity. Bioavailability of contaminants for nutrition and toxicity has opposite consequences which may have resulted in quite different bacterial adaptatio...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815274/ https://www.ncbi.nlm.nih.gov/pubmed/21951380 http://dx.doi.org/10.1111/j.1751-7915.2011.00300.x |
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author | Hanzel, Joanna Thullner, Martin Harms, Hauke Wick, Lukas Y. |
author_facet | Hanzel, Joanna Thullner, Martin Harms, Hauke Wick, Lukas Y. |
author_sort | Hanzel, Joanna |
collection | PubMed |
description | Microbial contaminant degradation may either result in the utilization of the compound for growth or act as a protective mechanism against its toxicity. Bioavailability of contaminants for nutrition and toxicity has opposite consequences which may have resulted in quite different bacterial adaptation mechanisms; these may particularly interfere when a growth substrate causes toxicity at high bioavailability. Recently, it has been demonstrated that a high bioavailability of vapour‐phase naphthalene (NAPH) leads to chemotactic movement of NAPH‐degrading Pseudomonas putida (NAH7) G7 away from the NAPH source. To investigate the balance of toxic defence and substrate utilization, we tested the influence of the cell density on surface‐associated growth of strain PpG7 at different positions in vapour‐phase NAPH gradients. Controlled microcosm experiments revealed that high cell densities increased growth rates close (< 2 cm) to the NAPH source, whereas competition for NAPH decreased the growth rates at larger distances despite the high gas phase diffusivity of NAPH. At larger distance, less microbial biomass was likewise sustained by the vapour‐phase NAPH. Such varying growth kinetics is explained by a combination of bioavailability restrictions and NAPH‐based inhibition. To account for this balance, a novel, integrated ‘Best Equation’ describing microbial growth influenced by substrate availability and inhibition is presented. |
format | Online Article Text |
id | pubmed-3815274 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-38152742014-02-12 Walking the tightrope of bioavailability: growth dynamics of PAH degraders on vapour‐phase PAH Hanzel, Joanna Thullner, Martin Harms, Hauke Wick, Lukas Y. Microb Biotechnol Research Articles Microbial contaminant degradation may either result in the utilization of the compound for growth or act as a protective mechanism against its toxicity. Bioavailability of contaminants for nutrition and toxicity has opposite consequences which may have resulted in quite different bacterial adaptation mechanisms; these may particularly interfere when a growth substrate causes toxicity at high bioavailability. Recently, it has been demonstrated that a high bioavailability of vapour‐phase naphthalene (NAPH) leads to chemotactic movement of NAPH‐degrading Pseudomonas putida (NAH7) G7 away from the NAPH source. To investigate the balance of toxic defence and substrate utilization, we tested the influence of the cell density on surface‐associated growth of strain PpG7 at different positions in vapour‐phase NAPH gradients. Controlled microcosm experiments revealed that high cell densities increased growth rates close (< 2 cm) to the NAPH source, whereas competition for NAPH decreased the growth rates at larger distances despite the high gas phase diffusivity of NAPH. At larger distance, less microbial biomass was likewise sustained by the vapour‐phase NAPH. Such varying growth kinetics is explained by a combination of bioavailability restrictions and NAPH‐based inhibition. To account for this balance, a novel, integrated ‘Best Equation’ describing microbial growth influenced by substrate availability and inhibition is presented. Blackwell Publishing Ltd 2012-01 2011-12-14 /pmc/articles/PMC3815274/ /pubmed/21951380 http://dx.doi.org/10.1111/j.1751-7915.2011.00300.x Text en Copyright © 2011 The Authors. Microbial Biotechnology © 2011 Society for Applied Microbiology and Blackwell Publishing Ltd |
spellingShingle | Research Articles Hanzel, Joanna Thullner, Martin Harms, Hauke Wick, Lukas Y. Walking the tightrope of bioavailability: growth dynamics of PAH degraders on vapour‐phase PAH |
title | Walking the tightrope of bioavailability: growth dynamics of PAH degraders on vapour‐phase PAH |
title_full | Walking the tightrope of bioavailability: growth dynamics of PAH degraders on vapour‐phase PAH |
title_fullStr | Walking the tightrope of bioavailability: growth dynamics of PAH degraders on vapour‐phase PAH |
title_full_unstemmed | Walking the tightrope of bioavailability: growth dynamics of PAH degraders on vapour‐phase PAH |
title_short | Walking the tightrope of bioavailability: growth dynamics of PAH degraders on vapour‐phase PAH |
title_sort | walking the tightrope of bioavailability: growth dynamics of pah degraders on vapour‐phase pah |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815274/ https://www.ncbi.nlm.nih.gov/pubmed/21951380 http://dx.doi.org/10.1111/j.1751-7915.2011.00300.x |
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