Intracellular monitoring of target protein production in Staphylococcus aureus by peptide tag‐induced reporter fluorescence

An intracellular approach for monitoring protein production in Staphylococcus aureus is described. mCherry, fused to the dodecapeptide Tip, was capable of inducing tetracycline repressor (TetR). Time‐ and concentration‐dependent production of mCherry could be correlated to TetR‐controlled GFPmut2 ac...

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Detalles Bibliográficos
Autores principales: Gauger, Tina, Weihs, Felix, Mayer, Sonja, Krismer, Bernhard, Liese, Jan, Kull, Melanie, Bertram, Ralph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2012
Materias:
Brief Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815279/
https://www.ncbi.nlm.nih.gov/pubmed/21958360
http://dx.doi.org/10.1111/j.1751-7915.2011.00304.x
Descripción
Sumario:An intracellular approach for monitoring protein production in Staphylococcus aureus is described. mCherry, fused to the dodecapeptide Tip, was capable of inducing tetracycline repressor (TetR). Time‐ and concentration‐dependent production of mCherry could be correlated to TetR‐controlled GFPmut2 activity. This approach can potentially be extended to native S. aureus proteins.

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