Intracellular monitoring of target protein production in Staphylococcus aureus by peptide tag‐induced reporter fluorescence

An intracellular approach for monitoring protein production in Staphylococcus aureus is described. mCherry, fused to the dodecapeptide Tip, was capable of inducing tetracycline repressor (TetR). Time‐ and concentration‐dependent production of mCherry could be correlated to TetR‐controlled GFPmut2 ac...

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Detalles Bibliográficos
Autores principales: Gauger, Tina, Weihs, Felix, Mayer, Sonja, Krismer, Bernhard, Liese, Jan, Kull, Melanie, Bertram, Ralph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2012
Materias:
Brief Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815279/
https://www.ncbi.nlm.nih.gov/pubmed/21958360
http://dx.doi.org/10.1111/j.1751-7915.2011.00304.x
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author Gauger, Tina
Weihs, Felix
Mayer, Sonja
Krismer, Bernhard
Liese, Jan
Kull, Melanie
Bertram, Ralph
author_facet Gauger, Tina
Weihs, Felix
Mayer, Sonja
Krismer, Bernhard
Liese, Jan
Kull, Melanie
Bertram, Ralph
author_sort Gauger, Tina
collection PubMed
description An intracellular approach for monitoring protein production in Staphylococcus aureus is described. mCherry, fused to the dodecapeptide Tip, was capable of inducing tetracycline repressor (TetR). Time‐ and concentration‐dependent production of mCherry could be correlated to TetR‐controlled GFPmut2 activity. This approach can potentially be extended to native S. aureus proteins.
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spelling pubmed-38152792014-02-12 Intracellular monitoring of target protein production in Staphylococcus aureus by peptide tag‐induced reporter fluorescence Gauger, Tina Weihs, Felix Mayer, Sonja Krismer, Bernhard Liese, Jan Kull, Melanie Bertram, Ralph Microb Biotechnol Brief Reports An intracellular approach for monitoring protein production in Staphylococcus aureus is described. mCherry, fused to the dodecapeptide Tip, was capable of inducing tetracycline repressor (TetR). Time‐ and concentration‐dependent production of mCherry could be correlated to TetR‐controlled GFPmut2 activity. This approach can potentially be extended to native S. aureus proteins. Blackwell Publishing Ltd 2012-01 2011-12-14 /pmc/articles/PMC3815279/ /pubmed/21958360 http://dx.doi.org/10.1111/j.1751-7915.2011.00304.x Text en Copyright © 2011 The Authors. Microbial Biotechnology © 2011 Society for Applied Microbiology and Blackwell Publishing Ltd
spellingShingle Brief Reports
Gauger, Tina
Weihs, Felix
Mayer, Sonja
Krismer, Bernhard
Liese, Jan
Kull, Melanie
Bertram, Ralph
Intracellular monitoring of target protein production in Staphylococcus aureus by peptide tag‐induced reporter fluorescence
title Intracellular monitoring of target protein production in Staphylococcus aureus by peptide tag‐induced reporter fluorescence
title_full Intracellular monitoring of target protein production in Staphylococcus aureus by peptide tag‐induced reporter fluorescence
title_fullStr Intracellular monitoring of target protein production in Staphylococcus aureus by peptide tag‐induced reporter fluorescence
title_full_unstemmed Intracellular monitoring of target protein production in Staphylococcus aureus by peptide tag‐induced reporter fluorescence
title_short Intracellular monitoring of target protein production in Staphylococcus aureus by peptide tag‐induced reporter fluorescence
title_sort intracellular monitoring of target protein production in staphylococcus aureus by peptide tag‐induced reporter fluorescence
topic Brief Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815279/
https://www.ncbi.nlm.nih.gov/pubmed/21958360
http://dx.doi.org/10.1111/j.1751-7915.2011.00304.x
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