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Strategies for enhancing bioluminescent bacterial sensor performance by promoter region manipulation
Bioluminescent bacterial sensors are based upon the fusion of bacterial bioluminescence (lux) genes, acting as a reporter element, to selected bacterial stress‐response gene promoters. Depending upon the nature of the promoter, the resulting constructs react to diverse types of environmental stress,...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815372/ https://www.ncbi.nlm.nih.gov/pubmed/21255329 http://dx.doi.org/10.1111/j.1751-7915.2009.00149.x |
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author | Yagur‐Kroll, Sharon Bilic, Benny Belkin, Shimshon |
author_facet | Yagur‐Kroll, Sharon Bilic, Benny Belkin, Shimshon |
author_sort | Yagur‐Kroll, Sharon |
collection | PubMed |
description | Bioluminescent bacterial sensors are based upon the fusion of bacterial bioluminescence (lux) genes, acting as a reporter element, to selected bacterial stress‐response gene promoters. Depending upon the nature of the promoter, the resulting constructs react to diverse types of environmental stress, including the presence of toxic chemicals, by dose‐dependant light emission. Two bacterial sensors, harbouring sulA::luxCDABE and grpE::luxCDABE fusions, activated by the model chemicals nalidixic acid (NA) and ethanol, respectively, were subjected to molecular manipulations of the promoter region, in order to enhance the intensity and speed of their response and lower their detection thresholds. By manipulating the length of the promoter‐containing segment (both promoters), by introducing random or specific mutations in the promoter sequence or by duplicating the promoter sequence (sulA only), major improvements in sensor performance were obtained. Improvements included significantly enhanced sensitivity, earlier response times and an increase in signal intensity. The general approaches described herein may be of general applicability for optimizing bacterial sensor performance, regardless of the sensing or reporting elements employed. |
format | Online Article Text |
id | pubmed-3815372 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-38153722014-02-12 Strategies for enhancing bioluminescent bacterial sensor performance by promoter region manipulation Yagur‐Kroll, Sharon Bilic, Benny Belkin, Shimshon Microb Biotechnol Research Articles Bioluminescent bacterial sensors are based upon the fusion of bacterial bioluminescence (lux) genes, acting as a reporter element, to selected bacterial stress‐response gene promoters. Depending upon the nature of the promoter, the resulting constructs react to diverse types of environmental stress, including the presence of toxic chemicals, by dose‐dependant light emission. Two bacterial sensors, harbouring sulA::luxCDABE and grpE::luxCDABE fusions, activated by the model chemicals nalidixic acid (NA) and ethanol, respectively, were subjected to molecular manipulations of the promoter region, in order to enhance the intensity and speed of their response and lower their detection thresholds. By manipulating the length of the promoter‐containing segment (both promoters), by introducing random or specific mutations in the promoter sequence or by duplicating the promoter sequence (sulA only), major improvements in sensor performance were obtained. Improvements included significantly enhanced sensitivity, earlier response times and an increase in signal intensity. The general approaches described herein may be of general applicability for optimizing bacterial sensor performance, regardless of the sensing or reporting elements employed. Blackwell Publishing Ltd 2010-05 2010-04-20 /pmc/articles/PMC3815372/ /pubmed/21255329 http://dx.doi.org/10.1111/j.1751-7915.2009.00149.x Text en Copyright© 2009 The Authors. Journal compilation © 2009 Society for Applied Microbiology and Blackwell Publishing Ltd |
spellingShingle | Research Articles Yagur‐Kroll, Sharon Bilic, Benny Belkin, Shimshon Strategies for enhancing bioluminescent bacterial sensor performance by promoter region manipulation |
title | Strategies for enhancing bioluminescent bacterial sensor performance by promoter region manipulation |
title_full | Strategies for enhancing bioluminescent bacterial sensor performance by promoter region manipulation |
title_fullStr | Strategies for enhancing bioluminescent bacterial sensor performance by promoter region manipulation |
title_full_unstemmed | Strategies for enhancing bioluminescent bacterial sensor performance by promoter region manipulation |
title_short | Strategies for enhancing bioluminescent bacterial sensor performance by promoter region manipulation |
title_sort | strategies for enhancing bioluminescent bacterial sensor performance by promoter region manipulation |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815372/ https://www.ncbi.nlm.nih.gov/pubmed/21255329 http://dx.doi.org/10.1111/j.1751-7915.2009.00149.x |
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