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Revolutionizing membrane protein overexpression in bacteria
The bacterium Escherichia coli is the most widely used expression host for overexpression trials of membrane proteins. Usually, different strains, culture conditions and expression regimes are screened for to identify the optimal overexpression strategy. However, yields are often not satisfactory, e...
Autores principales: | Schlegel, Susan, Klepsch, Mirjam, Gialama, Dimitra, Wickström, David, Slotboom, Dirk Jan, De Gier, Jan‐Willem |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815807/ https://www.ncbi.nlm.nih.gov/pubmed/21255339 http://dx.doi.org/10.1111/j.1751-7915.2009.00148.x |
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