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Assessing in situ rates of anaerobic hydrocarbon bioremediation

Identifying metabolites associated with anaerobic hydrocarbon biodegradation is a reliable way to garner evidence for the intrinsic bioremediation of problem contaminants. While such metabolites have been detected at numerous sites, the in situ rates of anaerobic hydrocarbon decay remain largely unk...

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Autores principales: Gieg, Lisa M., Alumbaugh, Robert E., Field, Jennifer, Jones, Jesse, Istok, Jonathon D., Suflita, Joseph M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815842/
https://www.ncbi.nlm.nih.gov/pubmed/21261916
http://dx.doi.org/10.1111/j.1751-7915.2008.00081.x
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author Gieg, Lisa M.
Alumbaugh, Robert E.
Field, Jennifer
Jones, Jesse
Istok, Jonathon D.
Suflita, Joseph M.
author_facet Gieg, Lisa M.
Alumbaugh, Robert E.
Field, Jennifer
Jones, Jesse
Istok, Jonathon D.
Suflita, Joseph M.
author_sort Gieg, Lisa M.
collection PubMed
description Identifying metabolites associated with anaerobic hydrocarbon biodegradation is a reliable way to garner evidence for the intrinsic bioremediation of problem contaminants. While such metabolites have been detected at numerous sites, the in situ rates of anaerobic hydrocarbon decay remain largely unknown. Yet, realistic rate information is critical for predicting how long individual contaminants will persist and remain environmental threats. Here, single‐well push–pull tests were conducted at two fuel‐contaminated aquifers to determine the in situ biotransformation rates of a suite of hydrocarbons added as deuterated surrogates, including toluene‐d(8), o‐xylene‐d(10), m‐xylene‐d(10), ethylbenzene‐d(5) (or ‐d(10)), 1, 2, 4‐trimethylbenzene‐d(12), 1, 3, 5‐trimethylbenzene‐d(12), methylcyclohexane‐d(14) and n‐hexane‐d(14). The formation of deuterated fumarate addition and downstream metabolites was quantified and found to be somewhat variable among wells in each aquifer, but generally within an order of magnitude. Deuterated metabolites formed in one aquifer at rates that ranged from 3 to 50 µg l(−1) day(−1), while the comparable rates at another aquifer were slower and ranged from 0.03 to 15 µg l(−1) day(−1). An important observation was that the deuterated hydrocarbon surrogates were metabolized in situ within hours or days at both sites, in contrast to many laboratory findings suggesting that long lag periods of weeks to months before the onset of anaerobic biodegradation are typical. It seems clear that highly reduced conditions are not detrimental to the intrinsic bioremediation of fuel‐contaminated aquifers.
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spelling pubmed-38158422014-02-12 Assessing in situ rates of anaerobic hydrocarbon bioremediation Gieg, Lisa M. Alumbaugh, Robert E. Field, Jennifer Jones, Jesse Istok, Jonathon D. Suflita, Joseph M. Microb Biotechnol Research Articles Identifying metabolites associated with anaerobic hydrocarbon biodegradation is a reliable way to garner evidence for the intrinsic bioremediation of problem contaminants. While such metabolites have been detected at numerous sites, the in situ rates of anaerobic hydrocarbon decay remain largely unknown. Yet, realistic rate information is critical for predicting how long individual contaminants will persist and remain environmental threats. Here, single‐well push–pull tests were conducted at two fuel‐contaminated aquifers to determine the in situ biotransformation rates of a suite of hydrocarbons added as deuterated surrogates, including toluene‐d(8), o‐xylene‐d(10), m‐xylene‐d(10), ethylbenzene‐d(5) (or ‐d(10)), 1, 2, 4‐trimethylbenzene‐d(12), 1, 3, 5‐trimethylbenzene‐d(12), methylcyclohexane‐d(14) and n‐hexane‐d(14). The formation of deuterated fumarate addition and downstream metabolites was quantified and found to be somewhat variable among wells in each aquifer, but generally within an order of magnitude. Deuterated metabolites formed in one aquifer at rates that ranged from 3 to 50 µg l(−1) day(−1), while the comparable rates at another aquifer were slower and ranged from 0.03 to 15 µg l(−1) day(−1). An important observation was that the deuterated hydrocarbon surrogates were metabolized in situ within hours or days at both sites, in contrast to many laboratory findings suggesting that long lag periods of weeks to months before the onset of anaerobic biodegradation are typical. It seems clear that highly reduced conditions are not detrimental to the intrinsic bioremediation of fuel‐contaminated aquifers. Blackwell Publishing Ltd 2009-03 2009-02-18 /pmc/articles/PMC3815842/ /pubmed/21261916 http://dx.doi.org/10.1111/j.1751-7915.2008.00081.x Text en © 2009 The Authors. Journal compilation © 2009 Society for Applied Microbiology and Blackwell Publishing Ltd
spellingShingle Research Articles
Gieg, Lisa M.
Alumbaugh, Robert E.
Field, Jennifer
Jones, Jesse
Istok, Jonathon D.
Suflita, Joseph M.
Assessing in situ rates of anaerobic hydrocarbon bioremediation
title Assessing in situ rates of anaerobic hydrocarbon bioremediation
title_full Assessing in situ rates of anaerobic hydrocarbon bioremediation
title_fullStr Assessing in situ rates of anaerobic hydrocarbon bioremediation
title_full_unstemmed Assessing in situ rates of anaerobic hydrocarbon bioremediation
title_short Assessing in situ rates of anaerobic hydrocarbon bioremediation
title_sort assessing in situ rates of anaerobic hydrocarbon bioremediation
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815842/
https://www.ncbi.nlm.nih.gov/pubmed/21261916
http://dx.doi.org/10.1111/j.1751-7915.2008.00081.x
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