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Induction of natural competence in Bacillus cereus ATCC14579

Natural competence is the ability of certain microbes to take up exogenous DNA from the environment and integrate it in their genome. Competence development has been described for a variety of bacteria, but has so far not been shown to occur in Bacillus cereus. However, orthologues of most proteins...

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Autores principales: Mirończuk, Aleksandra M., Kovács, Ákos T., Kuipers, Oscar P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815884/
https://www.ncbi.nlm.nih.gov/pubmed/21261842
http://dx.doi.org/10.1111/j.1751-7915.2008.00023.x
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author Mirończuk, Aleksandra M.
Kovács, Ákos T.
Kuipers, Oscar P.
author_facet Mirończuk, Aleksandra M.
Kovács, Ákos T.
Kuipers, Oscar P.
author_sort Mirończuk, Aleksandra M.
collection PubMed
description Natural competence is the ability of certain microbes to take up exogenous DNA from the environment and integrate it in their genome. Competence development has been described for a variety of bacteria, but has so far not been shown to occur in Bacillus cereus. However, orthologues of most proteins involved in natural DNA uptake in Bacillus subtiliscould be identified in B. cereus. Here, we report that B. cereus ATCC14579 can become naturally competent. When expressing the B. subtilis ComK protein using an IPTG‐inducible system in B. cereus ATCC14579, cells grown in minimal medium displayed natural competence, as either genomic DNA or plasmid DNA was shown to be taken up by the cells and integrated into the genome or stably maintained respectively. This work proves that a sufficient structural system for DNA uptake exists in B. cereus. Bacillus cereus can be employed as a model system to investigate the mechanism of DNA uptake in related bacteria such as Bacillus anthracis and Bacillus thuringiensis. Moreover, natural competence provides an important tool for biotechnology, as it will allow more efficient transformation of B. cereus and related organisms, e.g. to knockout genes in a high‐throughput way.
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spelling pubmed-38158842014-02-12 Induction of natural competence in Bacillus cereus ATCC14579 Mirończuk, Aleksandra M. Kovács, Ákos T. Kuipers, Oscar P. Microb Biotechnol Research Articles Natural competence is the ability of certain microbes to take up exogenous DNA from the environment and integrate it in their genome. Competence development has been described for a variety of bacteria, but has so far not been shown to occur in Bacillus cereus. However, orthologues of most proteins involved in natural DNA uptake in Bacillus subtiliscould be identified in B. cereus. Here, we report that B. cereus ATCC14579 can become naturally competent. When expressing the B. subtilis ComK protein using an IPTG‐inducible system in B. cereus ATCC14579, cells grown in minimal medium displayed natural competence, as either genomic DNA or plasmid DNA was shown to be taken up by the cells and integrated into the genome or stably maintained respectively. This work proves that a sufficient structural system for DNA uptake exists in B. cereus. Bacillus cereus can be employed as a model system to investigate the mechanism of DNA uptake in related bacteria such as Bacillus anthracis and Bacillus thuringiensis. Moreover, natural competence provides an important tool for biotechnology, as it will allow more efficient transformation of B. cereus and related organisms, e.g. to knockout genes in a high‐throughput way. Blackwell Publishing Ltd 2008-05 2008-02-01 /pmc/articles/PMC3815884/ /pubmed/21261842 http://dx.doi.org/10.1111/j.1751-7915.2008.00023.x Text en Copyright © 2008 The Authors. Journal compilation © 2008 Society for Applied Microbiology and Blackwell Publishing Ltd.
spellingShingle Research Articles
Mirończuk, Aleksandra M.
Kovács, Ákos T.
Kuipers, Oscar P.
Induction of natural competence in Bacillus cereus ATCC14579
title Induction of natural competence in Bacillus cereus ATCC14579
title_full Induction of natural competence in Bacillus cereus ATCC14579
title_fullStr Induction of natural competence in Bacillus cereus ATCC14579
title_full_unstemmed Induction of natural competence in Bacillus cereus ATCC14579
title_short Induction of natural competence in Bacillus cereus ATCC14579
title_sort induction of natural competence in bacillus cereus atcc14579
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815884/
https://www.ncbi.nlm.nih.gov/pubmed/21261842
http://dx.doi.org/10.1111/j.1751-7915.2008.00023.x
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