Rapid detection of total and viable Legionella pneumophila in tap water by immunomagnetic separation, double fluorescent staining and flow cytometry

We developed a rapid detection method for Legionella pneumophila (Lp) by filtration, immunomagnetic separation, double fluorescent staining, and flow cytometry (IMS‐FCM method). The method requires 120 min and can discriminate ‘viable’ and ‘membrane‐damaged’ cells. The recovery is over 85% of spiked...

Descripción completa

Detalles Bibliográficos
Autores principales: Keserue, Hans‐Anton, Baumgartner, Andreas, Felleisen, Richard, Egli, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing 2012
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815896/
https://www.ncbi.nlm.nih.gov/pubmed/23062200
http://dx.doi.org/10.1111/j.1751-7915.2012.00366.x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815896/
https://www.ncbi.nlm.nih.gov/pubmed/23062200
http://dx.doi.org/10.1111/j.1751-7915.2012.00366.x

Ejemplares similares