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Colonization process of olive tissues by Verticillium dahliae and its in planta interaction with the biocontrol root endophyte Pseudomonas fluorescens PICF7

The colonization process of Olea europaea by the defoliating pathotype of Verticillium dahliae, and the in planta interaction with the endophytic, biocontrol strain Pseudomonas fluorescens PICF7 were determined. Differential fluorescent protein tagging was used for the simultaneous visualization of...

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Autores principales: Prieto, Pilar, Navarro‐Raya, Carmen, Valverde‐Corredor, Antonio, Amyotte, Stefan G., Dobinson, Katherine F., Mercado‐Blanco, Jesús
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815910/
https://www.ncbi.nlm.nih.gov/pubmed/21255281
http://dx.doi.org/10.1111/j.1751-7915.2009.00105.x
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author Prieto, Pilar
Navarro‐Raya, Carmen
Valverde‐Corredor, Antonio
Amyotte, Stefan G.
Dobinson, Katherine F.
Mercado‐Blanco, Jesús
author_facet Prieto, Pilar
Navarro‐Raya, Carmen
Valverde‐Corredor, Antonio
Amyotte, Stefan G.
Dobinson, Katherine F.
Mercado‐Blanco, Jesús
author_sort Prieto, Pilar
collection PubMed
description The colonization process of Olea europaea by the defoliating pathotype of Verticillium dahliae, and the in planta interaction with the endophytic, biocontrol strain Pseudomonas fluorescens PICF7 were determined. Differential fluorescent protein tagging was used for the simultaneous visualization of P. fluorescens PICF7 and V. dahliae in olive tissues. Olive plants were bacterized with PICF7 and then transferred to V. dahliae‐infested soil. Monitoring olive colonization events by V. dahliae and its interaction with PICF7 was conducted using a non‐gnotobiotic system, confocal laser scanner microscopy and tissue vibratoming sections. A yellow fluorescently tagged V. dahliae derivative (VDAT‐36I) was obtained by Agrobacterium tumefaciens‐mediated transformation. Isolate VDAT‐36I quickly colonized olive root surface, successfully invaded root cortex and vascular tissues via macro‐ and micro‐breakages, and progressed to the aerial parts of the plant through xylem vessel cells. Strain PICF7 used root hairs as preferred penetration site, and once established on/in root tissues, hindered pathogen colonization. For the first time using this approach, the entire colonization process of a woody plant by V. dahliae is reported. Early and localized root surface and root endophytic colonization by P. fluorescens PICF7 is needed to impair full progress of verticillium wilt epidemics in olive.
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spelling pubmed-38159102014-02-12 Colonization process of olive tissues by Verticillium dahliae and its in planta interaction with the biocontrol root endophyte Pseudomonas fluorescens PICF7 Prieto, Pilar Navarro‐Raya, Carmen Valverde‐Corredor, Antonio Amyotte, Stefan G. Dobinson, Katherine F. Mercado‐Blanco, Jesús Microb Biotechnol Special Issue: Life of microbes that interact with plants
Guest Editors: Dr. Ana Segura, Dr. Gail Preston and Professor Pierre de Wit The colonization process of Olea europaea by the defoliating pathotype of Verticillium dahliae, and the in planta interaction with the endophytic, biocontrol strain Pseudomonas fluorescens PICF7 were determined. Differential fluorescent protein tagging was used for the simultaneous visualization of P. fluorescens PICF7 and V. dahliae in olive tissues. Olive plants were bacterized with PICF7 and then transferred to V. dahliae‐infested soil. Monitoring olive colonization events by V. dahliae and its interaction with PICF7 was conducted using a non‐gnotobiotic system, confocal laser scanner microscopy and tissue vibratoming sections. A yellow fluorescently tagged V. dahliae derivative (VDAT‐36I) was obtained by Agrobacterium tumefaciens‐mediated transformation. Isolate VDAT‐36I quickly colonized olive root surface, successfully invaded root cortex and vascular tissues via macro‐ and micro‐breakages, and progressed to the aerial parts of the plant through xylem vessel cells. Strain PICF7 used root hairs as preferred penetration site, and once established on/in root tissues, hindered pathogen colonization. For the first time using this approach, the entire colonization process of a woody plant by V. dahliae is reported. Early and localized root surface and root endophytic colonization by P. fluorescens PICF7 is needed to impair full progress of verticillium wilt epidemics in olive. Blackwell Publishing Ltd 2009-07 2009-06-15 /pmc/articles/PMC3815910/ /pubmed/21255281 http://dx.doi.org/10.1111/j.1751-7915.2009.00105.x Text en Copyright © 2009 The Authors. Journal compilation © 2009 Society for Applied Microbiology and Blackwell Publishing Ltd
spellingShingle Special Issue: Life of microbes that interact with plants
Guest Editors: Dr. Ana Segura, Dr. Gail Preston and Professor Pierre de Wit
Prieto, Pilar
Navarro‐Raya, Carmen
Valverde‐Corredor, Antonio
Amyotte, Stefan G.
Dobinson, Katherine F.
Mercado‐Blanco, Jesús
Colonization process of olive tissues by Verticillium dahliae and its in planta interaction with the biocontrol root endophyte Pseudomonas fluorescens PICF7
title Colonization process of olive tissues by Verticillium dahliae and its in planta interaction with the biocontrol root endophyte Pseudomonas fluorescens PICF7
title_full Colonization process of olive tissues by Verticillium dahliae and its in planta interaction with the biocontrol root endophyte Pseudomonas fluorescens PICF7
title_fullStr Colonization process of olive tissues by Verticillium dahliae and its in planta interaction with the biocontrol root endophyte Pseudomonas fluorescens PICF7
title_full_unstemmed Colonization process of olive tissues by Verticillium dahliae and its in planta interaction with the biocontrol root endophyte Pseudomonas fluorescens PICF7
title_short Colonization process of olive tissues by Verticillium dahliae and its in planta interaction with the biocontrol root endophyte Pseudomonas fluorescens PICF7
title_sort colonization process of olive tissues by verticillium dahliae and its in planta interaction with the biocontrol root endophyte pseudomonas fluorescens picf7
topic Special Issue: Life of microbes that interact with plants
Guest Editors: Dr. Ana Segura, Dr. Gail Preston and Professor Pierre de Wit
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3815910/
https://www.ncbi.nlm.nih.gov/pubmed/21255281
http://dx.doi.org/10.1111/j.1751-7915.2009.00105.x
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