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Depigmented Allergoids Reveal New Epitopes with Capacity to Induce IgG Blocking Antibodies
Background. The synthesis of allergen-specific blocking IgGs that interact with IgE after allergen immunotherapy (SIT) has been related to clinical efficacy. The objectives were to investigate the epitope specificity of IgG-antibodies induced by depigmented-polymerized (Dpg-Pol) allergoids and unmod...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3816019/ https://www.ncbi.nlm.nih.gov/pubmed/24222901 http://dx.doi.org/10.1155/2013/284615 |
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author | López-Matas, M. Angeles Gallego, Mayte Iraola, Víctor Robinson, Douglas Carnés, Jerónimo |
author_facet | López-Matas, M. Angeles Gallego, Mayte Iraola, Víctor Robinson, Douglas Carnés, Jerónimo |
author_sort | López-Matas, M. Angeles |
collection | PubMed |
description | Background. The synthesis of allergen-specific blocking IgGs that interact with IgE after allergen immunotherapy (SIT) has been related to clinical efficacy. The objectives were to investigate the epitope specificity of IgG-antibodies induced by depigmented-polymerized (Dpg-Pol) allergoids and unmodified allergen extracts, and examine IgE-blocking activity of induced IgG-antibodies. Methods. Rabbits were immunized with native and Dpg-Pol extracts of birch pollen, and serum samples were obtained. Recognition of linear IgG-epitopes of Bet v 1 and Bet v 2 and the capacity of these IgG-antibodies to block binding of human-IgE was determined. Results. Serum from rabbits immunized with native extracts recognised 11 linear epitopes from Bet v 1, while that from Dpg-Pol-immunized animals recognised 8. For Bet v 2, 8 epitopes were recognized by IgG from native immunized animals, and 9 from Dpg-Pol immunized one. Dpg-Pol and native immunized serum did not always recognise the same epitopes, but specific-IgG from both could block human-IgE binding sites for native extract. Conclusions. Depigmented-polymerized birch extract stimulates the synthesis of specific IgG-antibodies which recognize common but also novel epitopes compared with native extracts. IgG-antibodies induced by Dpg-Pol effectively inhibit human-IgE binding to allergens which may be part of the mechanism of action of SIT. |
format | Online Article Text |
id | pubmed-3816019 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-38160192013-11-11 Depigmented Allergoids Reveal New Epitopes with Capacity to Induce IgG Blocking Antibodies López-Matas, M. Angeles Gallego, Mayte Iraola, Víctor Robinson, Douglas Carnés, Jerónimo Biomed Res Int Research Article Background. The synthesis of allergen-specific blocking IgGs that interact with IgE after allergen immunotherapy (SIT) has been related to clinical efficacy. The objectives were to investigate the epitope specificity of IgG-antibodies induced by depigmented-polymerized (Dpg-Pol) allergoids and unmodified allergen extracts, and examine IgE-blocking activity of induced IgG-antibodies. Methods. Rabbits were immunized with native and Dpg-Pol extracts of birch pollen, and serum samples were obtained. Recognition of linear IgG-epitopes of Bet v 1 and Bet v 2 and the capacity of these IgG-antibodies to block binding of human-IgE was determined. Results. Serum from rabbits immunized with native extracts recognised 11 linear epitopes from Bet v 1, while that from Dpg-Pol-immunized animals recognised 8. For Bet v 2, 8 epitopes were recognized by IgG from native immunized animals, and 9 from Dpg-Pol immunized one. Dpg-Pol and native immunized serum did not always recognise the same epitopes, but specific-IgG from both could block human-IgE binding sites for native extract. Conclusions. Depigmented-polymerized birch extract stimulates the synthesis of specific IgG-antibodies which recognize common but also novel epitopes compared with native extracts. IgG-antibodies induced by Dpg-Pol effectively inhibit human-IgE binding to allergens which may be part of the mechanism of action of SIT. Hindawi Publishing Corporation 2013 2013-10-08 /pmc/articles/PMC3816019/ /pubmed/24222901 http://dx.doi.org/10.1155/2013/284615 Text en Copyright © 2013 M. Angeles López-Matas et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article López-Matas, M. Angeles Gallego, Mayte Iraola, Víctor Robinson, Douglas Carnés, Jerónimo Depigmented Allergoids Reveal New Epitopes with Capacity to Induce IgG Blocking Antibodies |
title | Depigmented Allergoids Reveal New Epitopes with Capacity to Induce IgG Blocking Antibodies |
title_full | Depigmented Allergoids Reveal New Epitopes with Capacity to Induce IgG Blocking Antibodies |
title_fullStr | Depigmented Allergoids Reveal New Epitopes with Capacity to Induce IgG Blocking Antibodies |
title_full_unstemmed | Depigmented Allergoids Reveal New Epitopes with Capacity to Induce IgG Blocking Antibodies |
title_short | Depigmented Allergoids Reveal New Epitopes with Capacity to Induce IgG Blocking Antibodies |
title_sort | depigmented allergoids reveal new epitopes with capacity to induce igg blocking antibodies |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3816019/ https://www.ncbi.nlm.nih.gov/pubmed/24222901 http://dx.doi.org/10.1155/2013/284615 |
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