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Effects of Resveratrol on Vitrified Porcine Oocytes

Vitrified MII porcine oocytes are characterized by reduced developmental competence, associated with the activation of the apoptotic pathway. Resveratrol (R), a polyphenolic compound present in several vegetal sources, has been reported to exert, among all its other biological effects, an antiapopto...

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Autores principales: Giaretta, Elisa, Spinaci, Marcella, Bucci, Diego, Tamanini, Carlo, Galeati, Giovanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3816072/
https://www.ncbi.nlm.nih.gov/pubmed/24223236
http://dx.doi.org/10.1155/2013/920257
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author Giaretta, Elisa
Spinaci, Marcella
Bucci, Diego
Tamanini, Carlo
Galeati, Giovanna
author_facet Giaretta, Elisa
Spinaci, Marcella
Bucci, Diego
Tamanini, Carlo
Galeati, Giovanna
author_sort Giaretta, Elisa
collection PubMed
description Vitrified MII porcine oocytes are characterized by reduced developmental competence, associated with the activation of the apoptotic pathway. Resveratrol (R), a polyphenolic compound present in several vegetal sources, has been reported to exert, among all its other biological effects, an antiapoptotic one. The aim of this study was to determine the effects of R (2 µM) on the apoptotic status of porcine oocytes vitrified by Cryotop method, evaluating phosphatidylserine (PS) exteriorization and caspases activation. R was added during IVM (A); 2 h postwarming incubation (B); vitrification/warming and 2 h postwarming incubation (C); all previous phases (D). Data on PS exteriorization showed, in each treated group, a significantly higher (P < 0.05) percentage of live nonapoptotic oocytes as compared with CTR; moreover, the percentage of live apoptotic oocytes was significantly (P < 0.05) lower in all R-treated groups relative to CTR. The results on caspase activation showed a tendency to an increase of viable oocytes with inactive caspases in B, C, and D, while a significant (P < 0.05) increase in A compared to CTR was recorded. These data demonstrate that R supplementation in various phases of IVM and vitrification/warming procedure can modulate the apoptotic process, improving the resistance of porcine oocytes to cryopreservation-induced damage.
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spelling pubmed-38160722013-11-12 Effects of Resveratrol on Vitrified Porcine Oocytes Giaretta, Elisa Spinaci, Marcella Bucci, Diego Tamanini, Carlo Galeati, Giovanna Oxid Med Cell Longev Research Article Vitrified MII porcine oocytes are characterized by reduced developmental competence, associated with the activation of the apoptotic pathway. Resveratrol (R), a polyphenolic compound present in several vegetal sources, has been reported to exert, among all its other biological effects, an antiapoptotic one. The aim of this study was to determine the effects of R (2 µM) on the apoptotic status of porcine oocytes vitrified by Cryotop method, evaluating phosphatidylserine (PS) exteriorization and caspases activation. R was added during IVM (A); 2 h postwarming incubation (B); vitrification/warming and 2 h postwarming incubation (C); all previous phases (D). Data on PS exteriorization showed, in each treated group, a significantly higher (P < 0.05) percentage of live nonapoptotic oocytes as compared with CTR; moreover, the percentage of live apoptotic oocytes was significantly (P < 0.05) lower in all R-treated groups relative to CTR. The results on caspase activation showed a tendency to an increase of viable oocytes with inactive caspases in B, C, and D, while a significant (P < 0.05) increase in A compared to CTR was recorded. These data demonstrate that R supplementation in various phases of IVM and vitrification/warming procedure can modulate the apoptotic process, improving the resistance of porcine oocytes to cryopreservation-induced damage. Hindawi Publishing Corporation 2013 2013-10-07 /pmc/articles/PMC3816072/ /pubmed/24223236 http://dx.doi.org/10.1155/2013/920257 Text en Copyright © 2013 Elisa Giaretta et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Giaretta, Elisa
Spinaci, Marcella
Bucci, Diego
Tamanini, Carlo
Galeati, Giovanna
Effects of Resveratrol on Vitrified Porcine Oocytes
title Effects of Resveratrol on Vitrified Porcine Oocytes
title_full Effects of Resveratrol on Vitrified Porcine Oocytes
title_fullStr Effects of Resveratrol on Vitrified Porcine Oocytes
title_full_unstemmed Effects of Resveratrol on Vitrified Porcine Oocytes
title_short Effects of Resveratrol on Vitrified Porcine Oocytes
title_sort effects of resveratrol on vitrified porcine oocytes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3816072/
https://www.ncbi.nlm.nih.gov/pubmed/24223236
http://dx.doi.org/10.1155/2013/920257
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