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Separation and characterization of nitrated variants of the major birch pollen allergen by CZE-ESI-μTOF MS

A CZE-ESI-TOF MS method has been optimized for the separation and identification of nitrated variants of the major birch pollen allergen from Betula verrucosa, isoform 1a (Bet v 1a). In-house nitration of recombinant Bet v 1a was done by peroxynitrite. As a BGE, 10 mmol/L ammonium bicarbonate with p...

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Detalles Bibliográficos
Autores principales:	Gusenkov, Sergey, Ackaert, Chloé, Stutz, Hanno
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Blackwell Publishing Ltd 2013
Materias:	Part IV: Proteins
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3816331/ https://www.ncbi.nlm.nih.gov/pubmed/23857337 http://dx.doi.org/10.1002/elps.201300151

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