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Control of gene expression by CRISPR-Cas systems

Clustered regularly interspaced short palindromic repeats (CRISPR) loci and their associated cas (CRISPR-associated) genes provide adaptive immunity against viruses (phages) and other mobile genetic elements in bacteria and archaea. While most of the early work has largely been dominated by examples...

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Detalles Bibliográficos
Autores principales: Bikard, David, Marraffini, Luciano A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Faculty of 1000 Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3816762/
https://www.ncbi.nlm.nih.gov/pubmed/24273648
http://dx.doi.org/10.12703/P5-47
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author Bikard, David
Marraffini, Luciano A.
author_facet Bikard, David
Marraffini, Luciano A.
author_sort Bikard, David
collection PubMed
description Clustered regularly interspaced short palindromic repeats (CRISPR) loci and their associated cas (CRISPR-associated) genes provide adaptive immunity against viruses (phages) and other mobile genetic elements in bacteria and archaea. While most of the early work has largely been dominated by examples of CRISPR-Cas systems directing the cleavage of phage or plasmid DNA, recent studies have revealed a more complex landscape where CRISPR-Cas loci might be involved in gene regulation. In this review, we summarize the role of these loci in the regulation of gene expression as well as the recent development of synthetic gene regulation using engineered CRISPR-Cas systems.
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spelling pubmed-38167622013-11-22 Control of gene expression by CRISPR-Cas systems Bikard, David Marraffini, Luciano A. F1000Prime Rep Review Article Clustered regularly interspaced short palindromic repeats (CRISPR) loci and their associated cas (CRISPR-associated) genes provide adaptive immunity against viruses (phages) and other mobile genetic elements in bacteria and archaea. While most of the early work has largely been dominated by examples of CRISPR-Cas systems directing the cleavage of phage or plasmid DNA, recent studies have revealed a more complex landscape where CRISPR-Cas loci might be involved in gene regulation. In this review, we summarize the role of these loci in the regulation of gene expression as well as the recent development of synthetic gene regulation using engineered CRISPR-Cas systems. Faculty of 1000 Ltd 2013-11-01 /pmc/articles/PMC3816762/ /pubmed/24273648 http://dx.doi.org/10.12703/P5-47 Text en © 2013 Faculty of 1000 Ltd http://creativecommons.org/licenses/by-nc/3.0/legalcode This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. You may not use this work for commercial purposes
spellingShingle Review Article
Bikard, David
Marraffini, Luciano A.
Control of gene expression by CRISPR-Cas systems
title Control of gene expression by CRISPR-Cas systems
title_full Control of gene expression by CRISPR-Cas systems
title_fullStr Control of gene expression by CRISPR-Cas systems
title_full_unstemmed Control of gene expression by CRISPR-Cas systems
title_short Control of gene expression by CRISPR-Cas systems
title_sort control of gene expression by crispr-cas systems
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3816762/
https://www.ncbi.nlm.nih.gov/pubmed/24273648
http://dx.doi.org/10.12703/P5-47
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