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Menaquinone and Iron Are Essential for Complex Colony Development in Bacillus subtilis
Cells of undomesticated species of Bacillus subtilis frequently form complex colonies during spreading on agar surfaces. Given that menaquinone is involved in another form of coordinated behavior, namely, sporulation, we looked for a possible role for menaquinone in complex colony development (CCD)...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3817097/ https://www.ncbi.nlm.nih.gov/pubmed/24223955 http://dx.doi.org/10.1371/journal.pone.0079488 |
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author | Pelchovich, Gidi Omer-Bendori, Shira Gophna, Uri |
author_facet | Pelchovich, Gidi Omer-Bendori, Shira Gophna, Uri |
author_sort | Pelchovich, Gidi |
collection | PubMed |
description | Cells of undomesticated species of Bacillus subtilis frequently form complex colonies during spreading on agar surfaces. Given that menaquinone is involved in another form of coordinated behavior, namely, sporulation, we looked for a possible role for menaquinone in complex colony development (CCD) in the B. subtilis strain NCIB 3610. Here we show that inhibition of menaquinone biosynthesis in B. subtilis indeed abolished its ability to develop complex colonies. Additionally some mutations of B. subtilis which confer defective CCD could be suppressed by menaquinone derivatives. Several such mutants mapped to the dhb operon encoding the genes responsible for the biosynthesis of the iron siderophore, bacillibactin. Our results demonstrate that both menaquinone and iron are essential for CCD in B. subtilis. |
format | Online Article Text |
id | pubmed-3817097 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-38170972013-11-09 Menaquinone and Iron Are Essential for Complex Colony Development in Bacillus subtilis Pelchovich, Gidi Omer-Bendori, Shira Gophna, Uri PLoS One Research Article Cells of undomesticated species of Bacillus subtilis frequently form complex colonies during spreading on agar surfaces. Given that menaquinone is involved in another form of coordinated behavior, namely, sporulation, we looked for a possible role for menaquinone in complex colony development (CCD) in the B. subtilis strain NCIB 3610. Here we show that inhibition of menaquinone biosynthesis in B. subtilis indeed abolished its ability to develop complex colonies. Additionally some mutations of B. subtilis which confer defective CCD could be suppressed by menaquinone derivatives. Several such mutants mapped to the dhb operon encoding the genes responsible for the biosynthesis of the iron siderophore, bacillibactin. Our results demonstrate that both menaquinone and iron are essential for CCD in B. subtilis. Public Library of Science 2013-11-04 /pmc/articles/PMC3817097/ /pubmed/24223955 http://dx.doi.org/10.1371/journal.pone.0079488 Text en © 2013 Pelchovich et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Pelchovich, Gidi Omer-Bendori, Shira Gophna, Uri Menaquinone and Iron Are Essential for Complex Colony Development in Bacillus subtilis |
title | Menaquinone and Iron Are Essential for Complex Colony Development in Bacillus subtilis
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title_full | Menaquinone and Iron Are Essential for Complex Colony Development in Bacillus subtilis
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title_fullStr | Menaquinone and Iron Are Essential for Complex Colony Development in Bacillus subtilis
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title_full_unstemmed | Menaquinone and Iron Are Essential for Complex Colony Development in Bacillus subtilis
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title_short | Menaquinone and Iron Are Essential for Complex Colony Development in Bacillus subtilis
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title_sort | menaquinone and iron are essential for complex colony development in bacillus subtilis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3817097/ https://www.ncbi.nlm.nih.gov/pubmed/24223955 http://dx.doi.org/10.1371/journal.pone.0079488 |
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