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Develop to Term Rat Oocytes Injected with Heat-Dried Sperm Heads

This study investigated the development of rat oocytes in vitro and in vivo following intracytoplasmic injection of heads from spermatozoa heat-dried at 50°C for 8 h and stored at 4°C in different gas phases. Sperm membrane and chromosome are damaged by the process of heat-drying. Oocyte activation...

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Autores principales: Lee, Kyung-Bon, Park, Ki-Eun, Kwon, In-Kiu, Tripurani, Swamy K., Kim, Keun Jung, Lee, Ji Hye, Niwa, Koji, Kim, Min Kyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3817241/
https://www.ncbi.nlm.nih.gov/pubmed/24223784
http://dx.doi.org/10.1371/journal.pone.0078260
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author Lee, Kyung-Bon
Park, Ki-Eun
Kwon, In-Kiu
Tripurani, Swamy K.
Kim, Keun Jung
Lee, Ji Hye
Niwa, Koji
Kim, Min Kyu
author_facet Lee, Kyung-Bon
Park, Ki-Eun
Kwon, In-Kiu
Tripurani, Swamy K.
Kim, Keun Jung
Lee, Ji Hye
Niwa, Koji
Kim, Min Kyu
author_sort Lee, Kyung-Bon
collection PubMed
description This study investigated the development of rat oocytes in vitro and in vivo following intracytoplasmic injection of heads from spermatozoa heat-dried at 50°C for 8 h and stored at 4°C in different gas phases. Sperm membrane and chromosome are damaged by the process of heat-drying. Oocyte activation and cleavage of oocytes were worse in oocytes injected with spermatozoa heat-dried and stored for 1 week than unheated, fresh spermatozoa, but in heat-dried spermatozoa, there were no differences in these abilities of oocytes between the samples stored in nitrogen gas and in air. The oocytes injected with heat-dried spermatozoa stored for 1 week could develop to the morula and blastocyst stages without difference between the samples stored in nitrogen gas and in air after artificial stimulation. Cleavage of oocytes and development of cleaved embryos were higher when heat-dried spermatozoa were stored for 3 and 6 months in nitrogen gas than in air. However, the ability of injected oocytes to develop to the morula and blastocyst stages was not inhibited even when heat-dried spermatozoa stored in both atmosphere conditions for as long as 6 months were used. When 2-cell embryos derived from oocytes injected with heads from spermatozoa heat-dried and stored for 1 week and 1 month were transferred, each 1 of 4 recipients was conceived, and the conceived recipients delivered 1 live young each. These results demonstrate that rat oocytes can be fertilized with heat-dried spermatozoa and that the fertilized oocytes can develop to term.
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spelling pubmed-38172412013-11-09 Develop to Term Rat Oocytes Injected with Heat-Dried Sperm Heads Lee, Kyung-Bon Park, Ki-Eun Kwon, In-Kiu Tripurani, Swamy K. Kim, Keun Jung Lee, Ji Hye Niwa, Koji Kim, Min Kyu PLoS One Research Article This study investigated the development of rat oocytes in vitro and in vivo following intracytoplasmic injection of heads from spermatozoa heat-dried at 50°C for 8 h and stored at 4°C in different gas phases. Sperm membrane and chromosome are damaged by the process of heat-drying. Oocyte activation and cleavage of oocytes were worse in oocytes injected with spermatozoa heat-dried and stored for 1 week than unheated, fresh spermatozoa, but in heat-dried spermatozoa, there were no differences in these abilities of oocytes between the samples stored in nitrogen gas and in air. The oocytes injected with heat-dried spermatozoa stored for 1 week could develop to the morula and blastocyst stages without difference between the samples stored in nitrogen gas and in air after artificial stimulation. Cleavage of oocytes and development of cleaved embryos were higher when heat-dried spermatozoa were stored for 3 and 6 months in nitrogen gas than in air. However, the ability of injected oocytes to develop to the morula and blastocyst stages was not inhibited even when heat-dried spermatozoa stored in both atmosphere conditions for as long as 6 months were used. When 2-cell embryos derived from oocytes injected with heads from spermatozoa heat-dried and stored for 1 week and 1 month were transferred, each 1 of 4 recipients was conceived, and the conceived recipients delivered 1 live young each. These results demonstrate that rat oocytes can be fertilized with heat-dried spermatozoa and that the fertilized oocytes can develop to term. Public Library of Science 2013-11-04 /pmc/articles/PMC3817241/ /pubmed/24223784 http://dx.doi.org/10.1371/journal.pone.0078260 Text en © 2013 Lee et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Lee, Kyung-Bon
Park, Ki-Eun
Kwon, In-Kiu
Tripurani, Swamy K.
Kim, Keun Jung
Lee, Ji Hye
Niwa, Koji
Kim, Min Kyu
Develop to Term Rat Oocytes Injected with Heat-Dried Sperm Heads
title Develop to Term Rat Oocytes Injected with Heat-Dried Sperm Heads
title_full Develop to Term Rat Oocytes Injected with Heat-Dried Sperm Heads
title_fullStr Develop to Term Rat Oocytes Injected with Heat-Dried Sperm Heads
title_full_unstemmed Develop to Term Rat Oocytes Injected with Heat-Dried Sperm Heads
title_short Develop to Term Rat Oocytes Injected with Heat-Dried Sperm Heads
title_sort develop to term rat oocytes injected with heat-dried sperm heads
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3817241/
https://www.ncbi.nlm.nih.gov/pubmed/24223784
http://dx.doi.org/10.1371/journal.pone.0078260
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