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Increase in Cellulose Accumulation and Improvement of Saccharification by Overexpression of Arabinofuranosidase in Rice

Cellulosic biomass is available for the production of biofuel, with saccharification of the cell wall being a key process. We investigated whether alteration of arabinoxylan, a major hemicellulose in monocots, causes an increase in saccharification efficiency. Arabinoxylans have β-1,4-D-xylopyranosy...

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Autores principales: Sumiyoshi, Minako, Nakamura, Atsuko, Nakamura, Hidemitsu, Hakata, Makoto, Ichikawa, Hiroaki, Hirochika, Hirohiko, Ishii, Tadashi, Satoh, Shinobu, Iwai, Hiroaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3817243/
https://www.ncbi.nlm.nih.gov/pubmed/24223786
http://dx.doi.org/10.1371/journal.pone.0078269
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author Sumiyoshi, Minako
Nakamura, Atsuko
Nakamura, Hidemitsu
Hakata, Makoto
Ichikawa, Hiroaki
Hirochika, Hirohiko
Ishii, Tadashi
Satoh, Shinobu
Iwai, Hiroaki
author_facet Sumiyoshi, Minako
Nakamura, Atsuko
Nakamura, Hidemitsu
Hakata, Makoto
Ichikawa, Hiroaki
Hirochika, Hirohiko
Ishii, Tadashi
Satoh, Shinobu
Iwai, Hiroaki
author_sort Sumiyoshi, Minako
collection PubMed
description Cellulosic biomass is available for the production of biofuel, with saccharification of the cell wall being a key process. We investigated whether alteration of arabinoxylan, a major hemicellulose in monocots, causes an increase in saccharification efficiency. Arabinoxylans have β-1,4-D-xylopyranosyl backbones and 1,3- or 1,4-α-l-arabinofuranosyl residues linked to O-2 and/or O-3 of xylopyranosyl residues as side chains. Arabinose side chains interrupt the hydrogen bond between arabinoxylan and cellulose and carry an ester-linked feruloyl substituent. Arabinose side chains are the base point for diferuloyl cross-links and lignification. We analyzed rice plants overexpressing arabinofuranosidase (ARAF) to study the role of arabinose residues in the cell wall and their effects on saccharification. Arabinose content in the cell wall of transgenic rice plants overexpressing individual ARAF full-length cDNA (OsARAF1-FOX and OsARAF3-FOX) decreased 25% and 20% compared to the control and the amount of glucose increased by 28.2% and 34.2%, respectively. We studied modifications of cell wall polysaccharides at the cellular level by comparing histochemical cellulose staining patterns and immunolocalization patterns using antibodies raised against α-(1,5)-linked l-Ara (LM6) and β-(1,4)-linked d-Xyl (LM10 and LM11) residues. However, they showed no visible phenotype. Our results suggest that the balance between arabinoxylan and cellulose might maintain the cell wall network. Moreover, ARAF overexpression in rice effectively leads to an increase in cellulose accumulation and saccharification efficiency, which can be used to produce bioethanol.
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spelling pubmed-38172432013-11-09 Increase in Cellulose Accumulation and Improvement of Saccharification by Overexpression of Arabinofuranosidase in Rice Sumiyoshi, Minako Nakamura, Atsuko Nakamura, Hidemitsu Hakata, Makoto Ichikawa, Hiroaki Hirochika, Hirohiko Ishii, Tadashi Satoh, Shinobu Iwai, Hiroaki PLoS One Research Article Cellulosic biomass is available for the production of biofuel, with saccharification of the cell wall being a key process. We investigated whether alteration of arabinoxylan, a major hemicellulose in monocots, causes an increase in saccharification efficiency. Arabinoxylans have β-1,4-D-xylopyranosyl backbones and 1,3- or 1,4-α-l-arabinofuranosyl residues linked to O-2 and/or O-3 of xylopyranosyl residues as side chains. Arabinose side chains interrupt the hydrogen bond between arabinoxylan and cellulose and carry an ester-linked feruloyl substituent. Arabinose side chains are the base point for diferuloyl cross-links and lignification. We analyzed rice plants overexpressing arabinofuranosidase (ARAF) to study the role of arabinose residues in the cell wall and their effects on saccharification. Arabinose content in the cell wall of transgenic rice plants overexpressing individual ARAF full-length cDNA (OsARAF1-FOX and OsARAF3-FOX) decreased 25% and 20% compared to the control and the amount of glucose increased by 28.2% and 34.2%, respectively. We studied modifications of cell wall polysaccharides at the cellular level by comparing histochemical cellulose staining patterns and immunolocalization patterns using antibodies raised against α-(1,5)-linked l-Ara (LM6) and β-(1,4)-linked d-Xyl (LM10 and LM11) residues. However, they showed no visible phenotype. Our results suggest that the balance between arabinoxylan and cellulose might maintain the cell wall network. Moreover, ARAF overexpression in rice effectively leads to an increase in cellulose accumulation and saccharification efficiency, which can be used to produce bioethanol. Public Library of Science 2013-11-04 /pmc/articles/PMC3817243/ /pubmed/24223786 http://dx.doi.org/10.1371/journal.pone.0078269 Text en © 2013 Sumiyoshi, et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Sumiyoshi, Minako
Nakamura, Atsuko
Nakamura, Hidemitsu
Hakata, Makoto
Ichikawa, Hiroaki
Hirochika, Hirohiko
Ishii, Tadashi
Satoh, Shinobu
Iwai, Hiroaki
Increase in Cellulose Accumulation and Improvement of Saccharification by Overexpression of Arabinofuranosidase in Rice
title Increase in Cellulose Accumulation and Improvement of Saccharification by Overexpression of Arabinofuranosidase in Rice
title_full Increase in Cellulose Accumulation and Improvement of Saccharification by Overexpression of Arabinofuranosidase in Rice
title_fullStr Increase in Cellulose Accumulation and Improvement of Saccharification by Overexpression of Arabinofuranosidase in Rice
title_full_unstemmed Increase in Cellulose Accumulation and Improvement of Saccharification by Overexpression of Arabinofuranosidase in Rice
title_short Increase in Cellulose Accumulation and Improvement of Saccharification by Overexpression of Arabinofuranosidase in Rice
title_sort increase in cellulose accumulation and improvement of saccharification by overexpression of arabinofuranosidase in rice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3817243/
https://www.ncbi.nlm.nih.gov/pubmed/24223786
http://dx.doi.org/10.1371/journal.pone.0078269
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