Cargando…

UPLC-TOF-MS Characterization and Identification of Bioactive Iridoids in Cornus mas Fruit

Cornus mas L. is indigenous to Europe and parts of Asia. Although Cornus is widely considered to be an iridoid rich genera, only two iridoids have been previously found in this plant. The lack of information on taxonomically and biologically active iridoids prompted us to develop and optimize an ana...

Descripción completa

Detalles Bibliográficos
Autores principales: Deng, Shixin, West, Brett J., Jensen, C. Jarakae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3817684/
https://www.ncbi.nlm.nih.gov/pubmed/24228188
http://dx.doi.org/10.1155/2013/710972
_version_ 1782478111219122176
author Deng, Shixin
West, Brett J.
Jensen, C. Jarakae
author_facet Deng, Shixin
West, Brett J.
Jensen, C. Jarakae
author_sort Deng, Shixin
collection PubMed
description Cornus mas L. is indigenous to Europe and parts of Asia. Although Cornus is widely considered to be an iridoid rich genera, only two iridoids have been previously found in this plant. The lack of information on taxonomically and biologically active iridoids prompted us to develop and optimize an analytical method for characterization of additional phytochemicals in C. mas fruit. An ultra performance liquid chromatography (UPLC) coupled with photodiode array spectrophotometry (PDA) and electrospray time-of-flight mass spectrometry (ESI-TOF-MS) was employed and mass parameters were optimized. Identification was made by elucidating the mass spectral data and further confirmed by comparing retention times and UV spectra of target peaks with those of reference compounds. Primary DNA damage and antigenotoxicity tests in E. coli PQ37 were used to screen the iridoids for biological activity. As a result, ten phytochemicals were identified, including iridoids loganic acid, loganin, sweroside, and cornuside. Nine of these were reported for the first time from C. mas fruit. The iridoids did not induce SOS repair of DNA, indicating a lack of genotoxic activity in E. coli PQ37. However, loganin, sweroside, and cornuside did reduce the amount of DNA damage caused by 4-nitroquinoline 1-oxide, suggesting potential antigenotoxic activity.
format Online
Article
Text
id pubmed-3817684
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-38176842013-11-13 UPLC-TOF-MS Characterization and Identification of Bioactive Iridoids in Cornus mas Fruit Deng, Shixin West, Brett J. Jensen, C. Jarakae J Anal Methods Chem Research Article Cornus mas L. is indigenous to Europe and parts of Asia. Although Cornus is widely considered to be an iridoid rich genera, only two iridoids have been previously found in this plant. The lack of information on taxonomically and biologically active iridoids prompted us to develop and optimize an analytical method for characterization of additional phytochemicals in C. mas fruit. An ultra performance liquid chromatography (UPLC) coupled with photodiode array spectrophotometry (PDA) and electrospray time-of-flight mass spectrometry (ESI-TOF-MS) was employed and mass parameters were optimized. Identification was made by elucidating the mass spectral data and further confirmed by comparing retention times and UV spectra of target peaks with those of reference compounds. Primary DNA damage and antigenotoxicity tests in E. coli PQ37 were used to screen the iridoids for biological activity. As a result, ten phytochemicals were identified, including iridoids loganic acid, loganin, sweroside, and cornuside. Nine of these were reported for the first time from C. mas fruit. The iridoids did not induce SOS repair of DNA, indicating a lack of genotoxic activity in E. coli PQ37. However, loganin, sweroside, and cornuside did reduce the amount of DNA damage caused by 4-nitroquinoline 1-oxide, suggesting potential antigenotoxic activity. Hindawi Publishing Corporation 2013 2013-10-08 /pmc/articles/PMC3817684/ /pubmed/24228188 http://dx.doi.org/10.1155/2013/710972 Text en Copyright © 2013 Shixin Deng et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Deng, Shixin
West, Brett J.
Jensen, C. Jarakae
UPLC-TOF-MS Characterization and Identification of Bioactive Iridoids in Cornus mas Fruit
title UPLC-TOF-MS Characterization and Identification of Bioactive Iridoids in Cornus mas Fruit
title_full UPLC-TOF-MS Characterization and Identification of Bioactive Iridoids in Cornus mas Fruit
title_fullStr UPLC-TOF-MS Characterization and Identification of Bioactive Iridoids in Cornus mas Fruit
title_full_unstemmed UPLC-TOF-MS Characterization and Identification of Bioactive Iridoids in Cornus mas Fruit
title_short UPLC-TOF-MS Characterization and Identification of Bioactive Iridoids in Cornus mas Fruit
title_sort uplc-tof-ms characterization and identification of bioactive iridoids in cornus mas fruit
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3817684/
https://www.ncbi.nlm.nih.gov/pubmed/24228188
http://dx.doi.org/10.1155/2013/710972
work_keys_str_mv AT dengshixin uplctofmscharacterizationandidentificationofbioactiveiridoidsincornusmasfruit
AT westbrettj uplctofmscharacterizationandidentificationofbioactiveiridoidsincornusmasfruit
AT jensencjarakae uplctofmscharacterizationandidentificationofbioactiveiridoidsincornusmasfruit