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Solving nucleic acid structures by molecular replacement: examples from group II intron studies

Structured RNA molecules are key players in ensuring cellular viability. It is now emerging that, like proteins, the functions of many nucleic acids are dictated by their tertiary folds. At the same time, the number of known crystal structures of nucleic acids is also increasing rapidly. In this con...

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Autores principales: Marcia, Marco, Humphris-Narayanan, Elisabeth, Keating, Kevin S., Somarowthu, Srinivas, Rajashankar, Kanagalaghatta, Pyle, Anna Marie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3817690/
https://www.ncbi.nlm.nih.gov/pubmed/24189228
http://dx.doi.org/10.1107/S0907444913013218
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author Marcia, Marco
Humphris-Narayanan, Elisabeth
Keating, Kevin S.
Somarowthu, Srinivas
Rajashankar, Kanagalaghatta
Pyle, Anna Marie
author_facet Marcia, Marco
Humphris-Narayanan, Elisabeth
Keating, Kevin S.
Somarowthu, Srinivas
Rajashankar, Kanagalaghatta
Pyle, Anna Marie
author_sort Marcia, Marco
collection PubMed
description Structured RNA molecules are key players in ensuring cellular viability. It is now emerging that, like proteins, the functions of many nucleic acids are dictated by their tertiary folds. At the same time, the number of known crystal structures of nucleic acids is also increasing rapidly. In this context, molecular replacement will become an increasingly useful technique for phasing nucleic acid crystallographic data in the near future. Here, strategies to select, create and refine molecular-replacement search models for nucleic acids are discussed. Using examples taken primarily from research on group II introns, it is shown that nucleic acids are amenable to different and potentially more flexible and sophisticated molecular-replacement searches than proteins. These observations specifically aim to encourage future crystallographic studies on the newly discovered repertoire of noncoding transcripts.
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spelling pubmed-38176902013-11-06 Solving nucleic acid structures by molecular replacement: examples from group II intron studies Marcia, Marco Humphris-Narayanan, Elisabeth Keating, Kevin S. Somarowthu, Srinivas Rajashankar, Kanagalaghatta Pyle, Anna Marie Acta Crystallogr D Biol Crystallogr Research Papers Structured RNA molecules are key players in ensuring cellular viability. It is now emerging that, like proteins, the functions of many nucleic acids are dictated by their tertiary folds. At the same time, the number of known crystal structures of nucleic acids is also increasing rapidly. In this context, molecular replacement will become an increasingly useful technique for phasing nucleic acid crystallographic data in the near future. Here, strategies to select, create and refine molecular-replacement search models for nucleic acids are discussed. Using examples taken primarily from research on group II introns, it is shown that nucleic acids are amenable to different and potentially more flexible and sophisticated molecular-replacement searches than proteins. These observations specifically aim to encourage future crystallographic studies on the newly discovered repertoire of noncoding transcripts. International Union of Crystallography 2013-11-01 2013-10-12 /pmc/articles/PMC3817690/ /pubmed/24189228 http://dx.doi.org/10.1107/S0907444913013218 Text en © Marcia et al. 2013 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
Marcia, Marco
Humphris-Narayanan, Elisabeth
Keating, Kevin S.
Somarowthu, Srinivas
Rajashankar, Kanagalaghatta
Pyle, Anna Marie
Solving nucleic acid structures by molecular replacement: examples from group II intron studies
title Solving nucleic acid structures by molecular replacement: examples from group II intron studies
title_full Solving nucleic acid structures by molecular replacement: examples from group II intron studies
title_fullStr Solving nucleic acid structures by molecular replacement: examples from group II intron studies
title_full_unstemmed Solving nucleic acid structures by molecular replacement: examples from group II intron studies
title_short Solving nucleic acid structures by molecular replacement: examples from group II intron studies
title_sort solving nucleic acid structures by molecular replacement: examples from group ii intron studies
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3817690/
https://www.ncbi.nlm.nih.gov/pubmed/24189228
http://dx.doi.org/10.1107/S0907444913013218
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