Cargando…
Solving nucleic acid structures by molecular replacement: examples from group II intron studies
Structured RNA molecules are key players in ensuring cellular viability. It is now emerging that, like proteins, the functions of many nucleic acids are dictated by their tertiary folds. At the same time, the number of known crystal structures of nucleic acids is also increasing rapidly. In this con...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3817690/ https://www.ncbi.nlm.nih.gov/pubmed/24189228 http://dx.doi.org/10.1107/S0907444913013218 |
_version_ | 1782478112520404992 |
---|---|
author | Marcia, Marco Humphris-Narayanan, Elisabeth Keating, Kevin S. Somarowthu, Srinivas Rajashankar, Kanagalaghatta Pyle, Anna Marie |
author_facet | Marcia, Marco Humphris-Narayanan, Elisabeth Keating, Kevin S. Somarowthu, Srinivas Rajashankar, Kanagalaghatta Pyle, Anna Marie |
author_sort | Marcia, Marco |
collection | PubMed |
description | Structured RNA molecules are key players in ensuring cellular viability. It is now emerging that, like proteins, the functions of many nucleic acids are dictated by their tertiary folds. At the same time, the number of known crystal structures of nucleic acids is also increasing rapidly. In this context, molecular replacement will become an increasingly useful technique for phasing nucleic acid crystallographic data in the near future. Here, strategies to select, create and refine molecular-replacement search models for nucleic acids are discussed. Using examples taken primarily from research on group II introns, it is shown that nucleic acids are amenable to different and potentially more flexible and sophisticated molecular-replacement searches than proteins. These observations specifically aim to encourage future crystallographic studies on the newly discovered repertoire of noncoding transcripts. |
format | Online Article Text |
id | pubmed-3817690 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-38176902013-11-06 Solving nucleic acid structures by molecular replacement: examples from group II intron studies Marcia, Marco Humphris-Narayanan, Elisabeth Keating, Kevin S. Somarowthu, Srinivas Rajashankar, Kanagalaghatta Pyle, Anna Marie Acta Crystallogr D Biol Crystallogr Research Papers Structured RNA molecules are key players in ensuring cellular viability. It is now emerging that, like proteins, the functions of many nucleic acids are dictated by their tertiary folds. At the same time, the number of known crystal structures of nucleic acids is also increasing rapidly. In this context, molecular replacement will become an increasingly useful technique for phasing nucleic acid crystallographic data in the near future. Here, strategies to select, create and refine molecular-replacement search models for nucleic acids are discussed. Using examples taken primarily from research on group II introns, it is shown that nucleic acids are amenable to different and potentially more flexible and sophisticated molecular-replacement searches than proteins. These observations specifically aim to encourage future crystallographic studies on the newly discovered repertoire of noncoding transcripts. International Union of Crystallography 2013-11-01 2013-10-12 /pmc/articles/PMC3817690/ /pubmed/24189228 http://dx.doi.org/10.1107/S0907444913013218 Text en © Marcia et al. 2013 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Research Papers Marcia, Marco Humphris-Narayanan, Elisabeth Keating, Kevin S. Somarowthu, Srinivas Rajashankar, Kanagalaghatta Pyle, Anna Marie Solving nucleic acid structures by molecular replacement: examples from group II intron studies |
title | Solving nucleic acid structures by molecular replacement: examples from group II intron studies |
title_full | Solving nucleic acid structures by molecular replacement: examples from group II intron studies |
title_fullStr | Solving nucleic acid structures by molecular replacement: examples from group II intron studies |
title_full_unstemmed | Solving nucleic acid structures by molecular replacement: examples from group II intron studies |
title_short | Solving nucleic acid structures by molecular replacement: examples from group II intron studies |
title_sort | solving nucleic acid structures by molecular replacement: examples from group ii intron studies |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3817690/ https://www.ncbi.nlm.nih.gov/pubmed/24189228 http://dx.doi.org/10.1107/S0907444913013218 |
work_keys_str_mv | AT marciamarco solvingnucleicacidstructuresbymolecularreplacementexamplesfromgroupiiintronstudies AT humphrisnarayananelisabeth solvingnucleicacidstructuresbymolecularreplacementexamplesfromgroupiiintronstudies AT keatingkevins solvingnucleicacidstructuresbymolecularreplacementexamplesfromgroupiiintronstudies AT somarowthusrinivas solvingnucleicacidstructuresbymolecularreplacementexamplesfromgroupiiintronstudies AT rajashankarkanagalaghatta solvingnucleicacidstructuresbymolecularreplacementexamplesfromgroupiiintronstudies AT pyleannamarie solvingnucleicacidstructuresbymolecularreplacementexamplesfromgroupiiintronstudies |