Cargando…
Zinc finger X-chromosomal protein promotes growth and tumorigenesis in human osteosarcoma cells
Objective: To investigate the role of Zinc finger X-chromosomal protein (ZFX) in oncogenesis of Osteosarcoma tumor. Methods: Here, we first conducted an expression analysis of ZFX in Osteosarcoma cell lines. Then, we constructed ZFX-specific small interfering RNA (siRNA)-lentiviral vector that is ca...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Professional Medical Publicaitons
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3817752/ https://www.ncbi.nlm.nih.gov/pubmed/24353675 |
_version_ | 1782478122067689472 |
---|---|
author | Jiang, Rui Gao, Zhong-li Sun, Mei Zhang, Xing-yi Wang, Jin-cheng Wu, Han |
author_facet | Jiang, Rui Gao, Zhong-li Sun, Mei Zhang, Xing-yi Wang, Jin-cheng Wu, Han |
author_sort | Jiang, Rui |
collection | PubMed |
description | Objective: To investigate the role of Zinc finger X-chromosomal protein (ZFX) in oncogenesis of Osteosarcoma tumor. Methods: Here, we first conducted an expression analysis of ZFX in Osteosarcoma cell lines. Then, we constructed ZFX-specific small interfering RNA (siRNA)-lentiviral vector that is capable of effectively inhibiting the expression of ZFX gene in human Osteosarcoma Saos-2 cells, and investigated systemically the impacts of ZFX silence on the growth and invasive ability of the cancer cells in vitro. Furthermore, we determined the effects of ZFX knockdown on the cell cycle distribution and apoptosis of Saos-2 cells. Results: We found that ZFX inhibition resulted in significantly impaired proliferation and colony formation as well as mitigated invasiveness of Saos-2 cells. Importantly, si-ZFX infected cells exhibited a greater portion of cells at G1 phase, but a minor portion of S and G2/M phase cells. Moreover, a greater portion of sub-G1 apoptotic cells was observed in si-ZFX infected cells. Conclusions: These results strongly suggest that ZFX is a novel proliferation regulator that promotes growth of Osteosarcoma cells, and downregulation of ZFX expression induces growth suppression of Saos-2 cells via arrested G0/G1 phase cell cycle and apoptosis pathways, thereby indicating that ZFX may serve as a new molecular target for Osteosarcoma tumor therapy. |
format | Online Article Text |
id | pubmed-3817752 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Professional Medical Publicaitons |
record_format | MEDLINE/PubMed |
spelling | pubmed-38177522013-12-18 Zinc finger X-chromosomal protein promotes growth and tumorigenesis in human osteosarcoma cells Jiang, Rui Gao, Zhong-li Sun, Mei Zhang, Xing-yi Wang, Jin-cheng Wu, Han Pak J Med Sci Original Article Objective: To investigate the role of Zinc finger X-chromosomal protein (ZFX) in oncogenesis of Osteosarcoma tumor. Methods: Here, we first conducted an expression analysis of ZFX in Osteosarcoma cell lines. Then, we constructed ZFX-specific small interfering RNA (siRNA)-lentiviral vector that is capable of effectively inhibiting the expression of ZFX gene in human Osteosarcoma Saos-2 cells, and investigated systemically the impacts of ZFX silence on the growth and invasive ability of the cancer cells in vitro. Furthermore, we determined the effects of ZFX knockdown on the cell cycle distribution and apoptosis of Saos-2 cells. Results: We found that ZFX inhibition resulted in significantly impaired proliferation and colony formation as well as mitigated invasiveness of Saos-2 cells. Importantly, si-ZFX infected cells exhibited a greater portion of cells at G1 phase, but a minor portion of S and G2/M phase cells. Moreover, a greater portion of sub-G1 apoptotic cells was observed in si-ZFX infected cells. Conclusions: These results strongly suggest that ZFX is a novel proliferation regulator that promotes growth of Osteosarcoma cells, and downregulation of ZFX expression induces growth suppression of Saos-2 cells via arrested G0/G1 phase cell cycle and apoptosis pathways, thereby indicating that ZFX may serve as a new molecular target for Osteosarcoma tumor therapy. Professional Medical Publicaitons 2013 /pmc/articles/PMC3817752/ /pubmed/24353675 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Jiang, Rui Gao, Zhong-li Sun, Mei Zhang, Xing-yi Wang, Jin-cheng Wu, Han Zinc finger X-chromosomal protein promotes growth and tumorigenesis in human osteosarcoma cells |
title | Zinc finger X-chromosomal protein promotes growth and tumorigenesis in human osteosarcoma cells |
title_full | Zinc finger X-chromosomal protein promotes growth and tumorigenesis in human osteosarcoma cells |
title_fullStr | Zinc finger X-chromosomal protein promotes growth and tumorigenesis in human osteosarcoma cells |
title_full_unstemmed | Zinc finger X-chromosomal protein promotes growth and tumorigenesis in human osteosarcoma cells |
title_short | Zinc finger X-chromosomal protein promotes growth and tumorigenesis in human osteosarcoma cells |
title_sort | zinc finger x-chromosomal protein promotes growth and tumorigenesis in human osteosarcoma cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3817752/ https://www.ncbi.nlm.nih.gov/pubmed/24353675 |
work_keys_str_mv | AT jiangrui zincfingerxchromosomalproteinpromotesgrowthandtumorigenesisinhumanosteosarcomacells AT gaozhongli zincfingerxchromosomalproteinpromotesgrowthandtumorigenesisinhumanosteosarcomacells AT sunmei zincfingerxchromosomalproteinpromotesgrowthandtumorigenesisinhumanosteosarcomacells AT zhangxingyi zincfingerxchromosomalproteinpromotesgrowthandtumorigenesisinhumanosteosarcomacells AT wangjincheng zincfingerxchromosomalproteinpromotesgrowthandtumorigenesisinhumanosteosarcomacells AT wuhan zincfingerxchromosomalproteinpromotesgrowthandtumorigenesisinhumanosteosarcomacells |