CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

Prokaryotic type II CRISPR-Cas systems can be adapted to enable targeted genome modifications across a range of eukaryotes.(1–7). Here we engineer this system to enable RNA-guided genome regulation in human cells by tethering transcriptional activation domains either directly to a nuclease-null Cas9...

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Detalles Bibliográficos
Autores principales: Mali, Prashant, Aach, John, Stranges, P. Benjamin, Esvelt, Kevin M., Moosburner, Mark, Kosuri, Sriram, Yang, Luhan, Church, George M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3818127/
https://www.ncbi.nlm.nih.gov/pubmed/23907171
http://dx.doi.org/10.1038/nbt.2675

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3818127/
https://www.ncbi.nlm.nih.gov/pubmed/23907171
http://dx.doi.org/10.1038/nbt.2675

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