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Extracellular Streptomyces vesicles: amphorae for survival and defence

Blue‐pigmented exudates arise as droplets on sporulated lawns of Streptomyces coelicolor M110 grown on agar plates. Our electron microscopical and biochemical studies suggest that droplets contain densely packed vesicles with large assemblies of different protein types and/or the polyketide antibiot...

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Autores principales: Schrempf, Hildgund, Koebsch, Ilona, Walter, Stefan, Engelhardt, Harald, Meschke, Holger
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3818868/
https://www.ncbi.nlm.nih.gov/pubmed/21342473
http://dx.doi.org/10.1111/j.1751-7915.2011.00251.x
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author Schrempf, Hildgund
Koebsch, Ilona
Walter, Stefan
Engelhardt, Harald
Meschke, Holger
author_facet Schrempf, Hildgund
Koebsch, Ilona
Walter, Stefan
Engelhardt, Harald
Meschke, Holger
author_sort Schrempf, Hildgund
collection PubMed
description Blue‐pigmented exudates arise as droplets on sporulated lawns of Streptomyces coelicolor M110 grown on agar plates. Our electron microscopical and biochemical studies suggest that droplets contain densely packed vesicles with large assemblies of different protein types and/or the polyketide antibiotic actinorhodin. Frozen‐hydrated vesicles were unilamellar with a typical bilayer membrane, and ranged from 80 to 400 nm in diameter with a preferred width of 150–300 nm. By means of cryo‐electron tomography, three types were reconstructed three‐dimensionally: vesicles that were filled with particulate material, likely protein assemblies, those that contained membrane‐bound particles, and a vesicle that showed a higher contrast inside, but lacked particles. Our LC/MS analyses of generated tryptic peptides led to the identification of distinct proteins that carry often a predicted N‐terminal signal peptide with a twin‐arginine motif or lack a canonical signal sequence. The proteins are required for a range of processes: the acquisition of inorganic as well as organic phosphate, iron ions, and of distinct carbon sources, energy metabolism and redox balance, defence against oxidants and tellurites, the tailoring of actinorhodin, folding and assembly of proteins, establishment of turgor, and different signalling cascades. Our novel findings have immense implications for understanding new avenues of environmental biology of streptomycetes and for biotechnological applications.
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spelling pubmed-38188682014-02-12 Extracellular Streptomyces vesicles: amphorae for survival and defence Schrempf, Hildgund Koebsch, Ilona Walter, Stefan Engelhardt, Harald Meschke, Holger Microb Biotechnol Research Articles Blue‐pigmented exudates arise as droplets on sporulated lawns of Streptomyces coelicolor M110 grown on agar plates. Our electron microscopical and biochemical studies suggest that droplets contain densely packed vesicles with large assemblies of different protein types and/or the polyketide antibiotic actinorhodin. Frozen‐hydrated vesicles were unilamellar with a typical bilayer membrane, and ranged from 80 to 400 nm in diameter with a preferred width of 150–300 nm. By means of cryo‐electron tomography, three types were reconstructed three‐dimensionally: vesicles that were filled with particulate material, likely protein assemblies, those that contained membrane‐bound particles, and a vesicle that showed a higher contrast inside, but lacked particles. Our LC/MS analyses of generated tryptic peptides led to the identification of distinct proteins that carry often a predicted N‐terminal signal peptide with a twin‐arginine motif or lack a canonical signal sequence. The proteins are required for a range of processes: the acquisition of inorganic as well as organic phosphate, iron ions, and of distinct carbon sources, energy metabolism and redox balance, defence against oxidants and tellurites, the tailoring of actinorhodin, folding and assembly of proteins, establishment of turgor, and different signalling cascades. Our novel findings have immense implications for understanding new avenues of environmental biology of streptomycetes and for biotechnological applications. Blackwell Publishing Ltd 2011-03 2011-02-22 /pmc/articles/PMC3818868/ /pubmed/21342473 http://dx.doi.org/10.1111/j.1751-7915.2011.00251.x Text en Copyright © 2011 The Authors. Journal compilation © 2011 Society for Applied Microbiology and Blackwell Publishing Ltd
spellingShingle Research Articles
Schrempf, Hildgund
Koebsch, Ilona
Walter, Stefan
Engelhardt, Harald
Meschke, Holger
Extracellular Streptomyces vesicles: amphorae for survival and defence
title Extracellular Streptomyces vesicles: amphorae for survival and defence
title_full Extracellular Streptomyces vesicles: amphorae for survival and defence
title_fullStr Extracellular Streptomyces vesicles: amphorae for survival and defence
title_full_unstemmed Extracellular Streptomyces vesicles: amphorae for survival and defence
title_short Extracellular Streptomyces vesicles: amphorae for survival and defence
title_sort extracellular streptomyces vesicles: amphorae for survival and defence
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3818868/
https://www.ncbi.nlm.nih.gov/pubmed/21342473
http://dx.doi.org/10.1111/j.1751-7915.2011.00251.x
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