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Three-Dimensional Reconstruction of Oral Tongue Squamous Cell Carcinoma at Invasion Front

We conducted three-dimensional (3D) reconstruction of oral tongue squamous cell carcinoma (OTSCC) using serial histological sections to visualize the architecture of invasive tumors. Fourteen OTSCC cases were collected from archival paraffin-embedded specimens. Based on a pathodiagnostic survey of w...

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Autores principales: Kudo, Tomoo, Shimazu, Yoshihito, Yagishita, Hisao, Izumo, Toshiyuki, Soeno, Yuuichi, Sato, Kaori, Taya, Yuji, Aoba, Takaaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3818895/
https://www.ncbi.nlm.nih.gov/pubmed/24228031
http://dx.doi.org/10.1155/2013/482765
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author Kudo, Tomoo
Shimazu, Yoshihito
Yagishita, Hisao
Izumo, Toshiyuki
Soeno, Yuuichi
Sato, Kaori
Taya, Yuji
Aoba, Takaaki
author_facet Kudo, Tomoo
Shimazu, Yoshihito
Yagishita, Hisao
Izumo, Toshiyuki
Soeno, Yuuichi
Sato, Kaori
Taya, Yuji
Aoba, Takaaki
author_sort Kudo, Tomoo
collection PubMed
description We conducted three-dimensional (3D) reconstruction of oral tongue squamous cell carcinoma (OTSCC) using serial histological sections to visualize the architecture of invasive tumors. Fourteen OTSCC cases were collected from archival paraffin-embedded specimens. Based on a pathodiagnostic survey of whole cancer lesions, a core tissue specimen (3 mm in diameter) was dissected out from the deep invasion front using a paraffin tissue microarray. Serial sections (4 μm thick) were double immunostained with pan-cytokeratin and Ki67 antibodies and digitized images were acquired using virtual microscopy. For 3D reconstruction, image registration and RGB color segmentation were automated using ImageJ software to avoid operator-dependent subjective errors. Based on the 3D tumor architecture, we classified the mode of invasion into four types: pushing and bulky architecture; trabecular architecture; diffuse spreading; and special forms. Direct visualization and quantitative assessment of the parenchymal-stromal border provide a new dimension in our understanding of OTSCC architecture. These 3D morphometric analyses also ascertained that cell invasion (individually and collectively) occurs at the deep invasive front of the OTSCC. These results demonstrate the advantages of histology-based 3D reconstruction for evaluating tumor architecture and its potential for a wide range of applications.
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spelling pubmed-38188952013-11-13 Three-Dimensional Reconstruction of Oral Tongue Squamous Cell Carcinoma at Invasion Front Kudo, Tomoo Shimazu, Yoshihito Yagishita, Hisao Izumo, Toshiyuki Soeno, Yuuichi Sato, Kaori Taya, Yuji Aoba, Takaaki Int J Dent Research Article We conducted three-dimensional (3D) reconstruction of oral tongue squamous cell carcinoma (OTSCC) using serial histological sections to visualize the architecture of invasive tumors. Fourteen OTSCC cases were collected from archival paraffin-embedded specimens. Based on a pathodiagnostic survey of whole cancer lesions, a core tissue specimen (3 mm in diameter) was dissected out from the deep invasion front using a paraffin tissue microarray. Serial sections (4 μm thick) were double immunostained with pan-cytokeratin and Ki67 antibodies and digitized images were acquired using virtual microscopy. For 3D reconstruction, image registration and RGB color segmentation were automated using ImageJ software to avoid operator-dependent subjective errors. Based on the 3D tumor architecture, we classified the mode of invasion into four types: pushing and bulky architecture; trabecular architecture; diffuse spreading; and special forms. Direct visualization and quantitative assessment of the parenchymal-stromal border provide a new dimension in our understanding of OTSCC architecture. These 3D morphometric analyses also ascertained that cell invasion (individually and collectively) occurs at the deep invasive front of the OTSCC. These results demonstrate the advantages of histology-based 3D reconstruction for evaluating tumor architecture and its potential for a wide range of applications. Hindawi Publishing Corporation 2013 2013-10-21 /pmc/articles/PMC3818895/ /pubmed/24228031 http://dx.doi.org/10.1155/2013/482765 Text en Copyright © 2013 Tomoo Kudo et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kudo, Tomoo
Shimazu, Yoshihito
Yagishita, Hisao
Izumo, Toshiyuki
Soeno, Yuuichi
Sato, Kaori
Taya, Yuji
Aoba, Takaaki
Three-Dimensional Reconstruction of Oral Tongue Squamous Cell Carcinoma at Invasion Front
title Three-Dimensional Reconstruction of Oral Tongue Squamous Cell Carcinoma at Invasion Front
title_full Three-Dimensional Reconstruction of Oral Tongue Squamous Cell Carcinoma at Invasion Front
title_fullStr Three-Dimensional Reconstruction of Oral Tongue Squamous Cell Carcinoma at Invasion Front
title_full_unstemmed Three-Dimensional Reconstruction of Oral Tongue Squamous Cell Carcinoma at Invasion Front
title_short Three-Dimensional Reconstruction of Oral Tongue Squamous Cell Carcinoma at Invasion Front
title_sort three-dimensional reconstruction of oral tongue squamous cell carcinoma at invasion front
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3818895/
https://www.ncbi.nlm.nih.gov/pubmed/24228031
http://dx.doi.org/10.1155/2013/482765
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