MicroRNA Target Site Identification by Integrating Sequence and Binding Information

High-throughput sequencing has opened numerous possibilities for the identification of regulatory RNA-binding events. Cross-linking and immunoprecipitation of Argonaute protein members can pinpoint microRNA target sites within tens of bases, but leaves the identity of the microRNA unresolved. A flex...

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Autores principales: Majoros, William H., Lekprasert, Parawee, Mukherjee, Neelanjan, Skalsky, Rebecca L., Corcoran, David L., Cullen, Bryan R., Ohler, Uwe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3818907/
https://www.ncbi.nlm.nih.gov/pubmed/23708386
http://dx.doi.org/10.1038/nmeth.2489
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author Majoros, William H.
Lekprasert, Parawee
Mukherjee, Neelanjan
Skalsky, Rebecca L.
Corcoran, David L.
Cullen, Bryan R.
Ohler, Uwe
author_facet Majoros, William H.
Lekprasert, Parawee
Mukherjee, Neelanjan
Skalsky, Rebecca L.
Corcoran, David L.
Cullen, Bryan R.
Ohler, Uwe
author_sort Majoros, William H.
collection PubMed
description High-throughput sequencing has opened numerous possibilities for the identification of regulatory RNA-binding events. Cross-linking and immunoprecipitation of Argonaute protein members can pinpoint microRNA target sites within tens of bases, but leaves the identity of the microRNA unresolved. A flexible computational framework that integrates sequence with cross-linking features reliably identifies the microRNA family involved in each binding event, considerably outperforms sequence-only approaches, and quantifies the prevalence of noncanonical binding modes.
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spelling pubmed-38189072014-01-01 MicroRNA Target Site Identification by Integrating Sequence and Binding Information Majoros, William H. Lekprasert, Parawee Mukherjee, Neelanjan Skalsky, Rebecca L. Corcoran, David L. Cullen, Bryan R. Ohler, Uwe Nat Methods Article High-throughput sequencing has opened numerous possibilities for the identification of regulatory RNA-binding events. Cross-linking and immunoprecipitation of Argonaute protein members can pinpoint microRNA target sites within tens of bases, but leaves the identity of the microRNA unresolved. A flexible computational framework that integrates sequence with cross-linking features reliably identifies the microRNA family involved in each binding event, considerably outperforms sequence-only approaches, and quantifies the prevalence of noncanonical binding modes. 2013-05-26 2013-07 /pmc/articles/PMC3818907/ /pubmed/23708386 http://dx.doi.org/10.1038/nmeth.2489 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Majoros, William H.
Lekprasert, Parawee
Mukherjee, Neelanjan
Skalsky, Rebecca L.
Corcoran, David L.
Cullen, Bryan R.
Ohler, Uwe
MicroRNA Target Site Identification by Integrating Sequence and Binding Information
title MicroRNA Target Site Identification by Integrating Sequence and Binding Information
title_full MicroRNA Target Site Identification by Integrating Sequence and Binding Information
title_fullStr MicroRNA Target Site Identification by Integrating Sequence and Binding Information
title_full_unstemmed MicroRNA Target Site Identification by Integrating Sequence and Binding Information
title_short MicroRNA Target Site Identification by Integrating Sequence and Binding Information
title_sort microrna target site identification by integrating sequence and binding information
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3818907/
https://www.ncbi.nlm.nih.gov/pubmed/23708386
http://dx.doi.org/10.1038/nmeth.2489
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