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Neuroprotective Effect of Ginkgolide B on Bupivacaine-Induced Apoptosis in SH-SY5Y Cells

Local anesthetics are used routinely and effectively. However, many are also known to activate neurotoxic pathways. We tested the neuroprotective efficacy of ginkgolide B (GB), an active component of Ginkgo biloba, against ROS-mediated neurotoxicity caused by the local anesthetic bupivacaine. SH-SY5...

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Autores principales: Li, Le, Zhang, Qing-guo, Lai, Lu-ying, Wen, Xian-jie, Zheng, Ting, Cheung, Chi-wai, Zhou, Shu-qin, Xu, Shi-yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3818975/
https://www.ncbi.nlm.nih.gov/pubmed/24228138
http://dx.doi.org/10.1155/2013/159864
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author Li, Le
Zhang, Qing-guo
Lai, Lu-ying
Wen, Xian-jie
Zheng, Ting
Cheung, Chi-wai
Zhou, Shu-qin
Xu, Shi-yuan
author_facet Li, Le
Zhang, Qing-guo
Lai, Lu-ying
Wen, Xian-jie
Zheng, Ting
Cheung, Chi-wai
Zhou, Shu-qin
Xu, Shi-yuan
author_sort Li, Le
collection PubMed
description Local anesthetics are used routinely and effectively. However, many are also known to activate neurotoxic pathways. We tested the neuroprotective efficacy of ginkgolide B (GB), an active component of Ginkgo biloba, against ROS-mediated neurotoxicity caused by the local anesthetic bupivacaine. SH-SY5Y cells were treated with different concentrations of bupivacaine alone or following preincubation with GB. Pretreatment with GB increased SH-SY5Y cell viability and attenuated intracellular ROS accumulation, apoptosis, mitochondrial dysfunction, and ER stress. GB suppressed bupivacaine-induced mitochondrial depolarization and mitochondria complex I and III inhibition and increased cleaved caspase-3 and Htra2 expression, which was strongly indicative of activation of mitochondria-dependent apoptosis with concomitantly enhanced expressions of Grp78, caspase-12 mRNA, protein, and ER stress. GB also improved ultrastructural changes indicative of mitochondrial and ER damage induced by bupivacaine. These results implicate bupivacaine-induced ROS-dependent mitochondria, ER dysfunction, and apoptosis, which can be attenuated by GB through its antioxidant property.
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spelling pubmed-38189752013-11-13 Neuroprotective Effect of Ginkgolide B on Bupivacaine-Induced Apoptosis in SH-SY5Y Cells Li, Le Zhang, Qing-guo Lai, Lu-ying Wen, Xian-jie Zheng, Ting Cheung, Chi-wai Zhou, Shu-qin Xu, Shi-yuan Oxid Med Cell Longev Research Article Local anesthetics are used routinely and effectively. However, many are also known to activate neurotoxic pathways. We tested the neuroprotective efficacy of ginkgolide B (GB), an active component of Ginkgo biloba, against ROS-mediated neurotoxicity caused by the local anesthetic bupivacaine. SH-SY5Y cells were treated with different concentrations of bupivacaine alone or following preincubation with GB. Pretreatment with GB increased SH-SY5Y cell viability and attenuated intracellular ROS accumulation, apoptosis, mitochondrial dysfunction, and ER stress. GB suppressed bupivacaine-induced mitochondrial depolarization and mitochondria complex I and III inhibition and increased cleaved caspase-3 and Htra2 expression, which was strongly indicative of activation of mitochondria-dependent apoptosis with concomitantly enhanced expressions of Grp78, caspase-12 mRNA, protein, and ER stress. GB also improved ultrastructural changes indicative of mitochondrial and ER damage induced by bupivacaine. These results implicate bupivacaine-induced ROS-dependent mitochondria, ER dysfunction, and apoptosis, which can be attenuated by GB through its antioxidant property. Hindawi Publishing Corporation 2013 2013-10-21 /pmc/articles/PMC3818975/ /pubmed/24228138 http://dx.doi.org/10.1155/2013/159864 Text en Copyright © 2013 Le Li et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Le
Zhang, Qing-guo
Lai, Lu-ying
Wen, Xian-jie
Zheng, Ting
Cheung, Chi-wai
Zhou, Shu-qin
Xu, Shi-yuan
Neuroprotective Effect of Ginkgolide B on Bupivacaine-Induced Apoptosis in SH-SY5Y Cells
title Neuroprotective Effect of Ginkgolide B on Bupivacaine-Induced Apoptosis in SH-SY5Y Cells
title_full Neuroprotective Effect of Ginkgolide B on Bupivacaine-Induced Apoptosis in SH-SY5Y Cells
title_fullStr Neuroprotective Effect of Ginkgolide B on Bupivacaine-Induced Apoptosis in SH-SY5Y Cells
title_full_unstemmed Neuroprotective Effect of Ginkgolide B on Bupivacaine-Induced Apoptosis in SH-SY5Y Cells
title_short Neuroprotective Effect of Ginkgolide B on Bupivacaine-Induced Apoptosis in SH-SY5Y Cells
title_sort neuroprotective effect of ginkgolide b on bupivacaine-induced apoptosis in sh-sy5y cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3818975/
https://www.ncbi.nlm.nih.gov/pubmed/24228138
http://dx.doi.org/10.1155/2013/159864
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