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Metabolism of a plant derived galactose‐containing polysaccharide by Bifidobacterium breve UCC2003
In this study, we describe the functional characterization of the Bifidobacterium breve UCC2003 gal locus, which is dedicated to the utilization of galactan, a plant‐derived polysaccharide. Using a combination of molecular approaches we conclude that the galA gene of B. breve UCC2003 encodes a β‐1,4...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3818998/ https://www.ncbi.nlm.nih.gov/pubmed/21375716 http://dx.doi.org/10.1111/j.1751-7915.2010.00218.x |
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author | O'Connell Motherway, Mary Fitzgerald, Gerald F. van Sinderen, Douwe |
author_facet | O'Connell Motherway, Mary Fitzgerald, Gerald F. van Sinderen, Douwe |
author_sort | O'Connell Motherway, Mary |
collection | PubMed |
description | In this study, we describe the functional characterization of the Bifidobacterium breve UCC2003 gal locus, which is dedicated to the utilization of galactan, a plant‐derived polysaccharide. Using a combination of molecular approaches we conclude that the galA gene of B. breve UCC2003 encodes a β‐1,4‐endogalactanase producing galacto‐oligosaccharides, which are specifically internalized by an ABC transport system, encoded by galBCDE, and which are then hydrolysed to galactose moieties by a dedicated intracellular β‐galactosidase, specified by galG. The generated galactose molecules are presumed to be fed into the fructose‐6‐phosphate phosphoketolase pathway via the Leloir pathway, thereby allowing B. breve UCC2003 to use galactan as its sole carbon and energy source. In addition to these findings we demonstrate that GalR is a LacI‐type DNA‐binding protein, which not only appears to control transcription of the galCDEGR operon, but also that of the galA gene. |
format | Online Article Text |
id | pubmed-3818998 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-38189982014-02-12 Metabolism of a plant derived galactose‐containing polysaccharide by Bifidobacterium breve UCC2003 O'Connell Motherway, Mary Fitzgerald, Gerald F. van Sinderen, Douwe Microb Biotechnol Research Articles In this study, we describe the functional characterization of the Bifidobacterium breve UCC2003 gal locus, which is dedicated to the utilization of galactan, a plant‐derived polysaccharide. Using a combination of molecular approaches we conclude that the galA gene of B. breve UCC2003 encodes a β‐1,4‐endogalactanase producing galacto‐oligosaccharides, which are specifically internalized by an ABC transport system, encoded by galBCDE, and which are then hydrolysed to galactose moieties by a dedicated intracellular β‐galactosidase, specified by galG. The generated galactose molecules are presumed to be fed into the fructose‐6‐phosphate phosphoketolase pathway via the Leloir pathway, thereby allowing B. breve UCC2003 to use galactan as its sole carbon and energy source. In addition to these findings we demonstrate that GalR is a LacI‐type DNA‐binding protein, which not only appears to control transcription of the galCDEGR operon, but also that of the galA gene. Blackwell Publishing Ltd 2011-05 2011-04-25 /pmc/articles/PMC3818998/ /pubmed/21375716 http://dx.doi.org/10.1111/j.1751-7915.2010.00218.x Text en Copyright © 2010 University College Cork. Journal compilation © 2010 Society for Applied Microbiology and Blackwell Publishing Ltd |
spellingShingle | Research Articles O'Connell Motherway, Mary Fitzgerald, Gerald F. van Sinderen, Douwe Metabolism of a plant derived galactose‐containing polysaccharide by Bifidobacterium breve UCC2003 |
title | Metabolism of a plant derived galactose‐containing polysaccharide by Bifidobacterium breve UCC2003 |
title_full | Metabolism of a plant derived galactose‐containing polysaccharide by Bifidobacterium breve UCC2003 |
title_fullStr | Metabolism of a plant derived galactose‐containing polysaccharide by Bifidobacterium breve UCC2003 |
title_full_unstemmed | Metabolism of a plant derived galactose‐containing polysaccharide by Bifidobacterium breve UCC2003 |
title_short | Metabolism of a plant derived galactose‐containing polysaccharide by Bifidobacterium breve UCC2003 |
title_sort | metabolism of a plant derived galactose‐containing polysaccharide by bifidobacterium breve ucc2003 |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3818998/ https://www.ncbi.nlm.nih.gov/pubmed/21375716 http://dx.doi.org/10.1111/j.1751-7915.2010.00218.x |
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