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A novel screening system for secretion of heterologous proteins in Bacillus subtilis

High‐level production of secretory proteins in Bacillus subtilis leads to a stress response involving the two‐component system CssRS and its target genes htrA and htrB. Here, we used this sensing system in a reporter strain in which gfp is under control of P(htrA), the secretion stress responsive pr...

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Detalles Bibliográficos
Autores principales: Trip, Hein, van der Veek, Patricia J., Renniers, Ton C., Meima, Rob, Sagt, Cees M., Mohrmann, Lisette, Kuipers, Oscar P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3819016/
https://www.ncbi.nlm.nih.gov/pubmed/21624103
http://dx.doi.org/10.1111/j.1751-7915.2011.00270.x
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author Trip, Hein
van der Veek, Patricia J.
Renniers, Ton C.
Meima, Rob
Sagt, Cees M.
Mohrmann, Lisette
Kuipers, Oscar P.
author_facet Trip, Hein
van der Veek, Patricia J.
Renniers, Ton C.
Meima, Rob
Sagt, Cees M.
Mohrmann, Lisette
Kuipers, Oscar P.
author_sort Trip, Hein
collection PubMed
description High‐level production of secretory proteins in Bacillus subtilis leads to a stress response involving the two‐component system CssRS and its target genes htrA and htrB. Here, we used this sensing system in a reporter strain in which gfp is under control of P(htrA), the secretion stress responsive promoter of htrA. Overexpression of heterologous secretory proteins in this strain results in green fluorescent cells, which can be separated from non‐secreting, low fluorescent cells using a fluorescence‐activated cell sorter (FACS). Using this principle, genomic libraries of uncharacterized prokaryotic organisms, expressed in the reporter strain, can be screened for genes encoding secretory proteins.
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spelling pubmed-38190162014-02-12 A novel screening system for secretion of heterologous proteins in Bacillus subtilis Trip, Hein van der Veek, Patricia J. Renniers, Ton C. Meima, Rob Sagt, Cees M. Mohrmann, Lisette Kuipers, Oscar P. Microb Biotechnol Research Articles High‐level production of secretory proteins in Bacillus subtilis leads to a stress response involving the two‐component system CssRS and its target genes htrA and htrB. Here, we used this sensing system in a reporter strain in which gfp is under control of P(htrA), the secretion stress responsive promoter of htrA. Overexpression of heterologous secretory proteins in this strain results in green fluorescent cells, which can be separated from non‐secreting, low fluorescent cells using a fluorescence‐activated cell sorter (FACS). Using this principle, genomic libraries of uncharacterized prokaryotic organisms, expressed in the reporter strain, can be screened for genes encoding secretory proteins. Blackwell Publishing Ltd 2011-09 2011-08-17 /pmc/articles/PMC3819016/ /pubmed/21624103 http://dx.doi.org/10.1111/j.1751-7915.2011.00270.x Text en Copyright © 2011 The Authors. Journal compilation © 2011 Society for Applied Microbiology and Blackwell Publishing Ltd
spellingShingle Research Articles
Trip, Hein
van der Veek, Patricia J.
Renniers, Ton C.
Meima, Rob
Sagt, Cees M.
Mohrmann, Lisette
Kuipers, Oscar P.
A novel screening system for secretion of heterologous proteins in Bacillus subtilis
title A novel screening system for secretion of heterologous proteins in Bacillus subtilis
title_full A novel screening system for secretion of heterologous proteins in Bacillus subtilis
title_fullStr A novel screening system for secretion of heterologous proteins in Bacillus subtilis
title_full_unstemmed A novel screening system for secretion of heterologous proteins in Bacillus subtilis
title_short A novel screening system for secretion of heterologous proteins in Bacillus subtilis
title_sort novel screening system for secretion of heterologous proteins in bacillus subtilis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3819016/
https://www.ncbi.nlm.nih.gov/pubmed/21624103
http://dx.doi.org/10.1111/j.1751-7915.2011.00270.x
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