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A novel screening system for secretion of heterologous proteins in Bacillus subtilis
High‐level production of secretory proteins in Bacillus subtilis leads to a stress response involving the two‐component system CssRS and its target genes htrA and htrB. Here, we used this sensing system in a reporter strain in which gfp is under control of P(htrA), the secretion stress responsive pr...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3819016/ https://www.ncbi.nlm.nih.gov/pubmed/21624103 http://dx.doi.org/10.1111/j.1751-7915.2011.00270.x |
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author | Trip, Hein van der Veek, Patricia J. Renniers, Ton C. Meima, Rob Sagt, Cees M. Mohrmann, Lisette Kuipers, Oscar P. |
author_facet | Trip, Hein van der Veek, Patricia J. Renniers, Ton C. Meima, Rob Sagt, Cees M. Mohrmann, Lisette Kuipers, Oscar P. |
author_sort | Trip, Hein |
collection | PubMed |
description | High‐level production of secretory proteins in Bacillus subtilis leads to a stress response involving the two‐component system CssRS and its target genes htrA and htrB. Here, we used this sensing system in a reporter strain in which gfp is under control of P(htrA), the secretion stress responsive promoter of htrA. Overexpression of heterologous secretory proteins in this strain results in green fluorescent cells, which can be separated from non‐secreting, low fluorescent cells using a fluorescence‐activated cell sorter (FACS). Using this principle, genomic libraries of uncharacterized prokaryotic organisms, expressed in the reporter strain, can be screened for genes encoding secretory proteins. |
format | Online Article Text |
id | pubmed-3819016 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Blackwell Publishing Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-38190162014-02-12 A novel screening system for secretion of heterologous proteins in Bacillus subtilis Trip, Hein van der Veek, Patricia J. Renniers, Ton C. Meima, Rob Sagt, Cees M. Mohrmann, Lisette Kuipers, Oscar P. Microb Biotechnol Research Articles High‐level production of secretory proteins in Bacillus subtilis leads to a stress response involving the two‐component system CssRS and its target genes htrA and htrB. Here, we used this sensing system in a reporter strain in which gfp is under control of P(htrA), the secretion stress responsive promoter of htrA. Overexpression of heterologous secretory proteins in this strain results in green fluorescent cells, which can be separated from non‐secreting, low fluorescent cells using a fluorescence‐activated cell sorter (FACS). Using this principle, genomic libraries of uncharacterized prokaryotic organisms, expressed in the reporter strain, can be screened for genes encoding secretory proteins. Blackwell Publishing Ltd 2011-09 2011-08-17 /pmc/articles/PMC3819016/ /pubmed/21624103 http://dx.doi.org/10.1111/j.1751-7915.2011.00270.x Text en Copyright © 2011 The Authors. Journal compilation © 2011 Society for Applied Microbiology and Blackwell Publishing Ltd |
spellingShingle | Research Articles Trip, Hein van der Veek, Patricia J. Renniers, Ton C. Meima, Rob Sagt, Cees M. Mohrmann, Lisette Kuipers, Oscar P. A novel screening system for secretion of heterologous proteins in Bacillus subtilis |
title | A novel screening system for secretion of heterologous proteins in Bacillus subtilis |
title_full | A novel screening system for secretion of heterologous proteins in Bacillus subtilis |
title_fullStr | A novel screening system for secretion of heterologous proteins in Bacillus subtilis |
title_full_unstemmed | A novel screening system for secretion of heterologous proteins in Bacillus subtilis |
title_short | A novel screening system for secretion of heterologous proteins in Bacillus subtilis |
title_sort | novel screening system for secretion of heterologous proteins in bacillus subtilis |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3819016/ https://www.ncbi.nlm.nih.gov/pubmed/21624103 http://dx.doi.org/10.1111/j.1751-7915.2011.00270.x |
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