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Diversified Microbiota of Meconium Is Affected by Maternal Diabetes Status

OBJECTIVES: This study was aimed to assess the diversity of the meconium microbiome and determine if the bacterial community is affected by maternal diabetes status. METHODS: The first intestinal discharge (meconium) was collected from 23 newborns stratified by maternal diabetes status: 4 mothers ha...

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Autores principales: Hu, Jianzhong, Nomura, Yoko, Bashir, Ali, Fernandez-Hernandez, Heriberto, Itzkowitz, Steven, Pei, Zhiheng, Stone, Joanne, Loudon, Holly, Peter, Inga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3819383/
https://www.ncbi.nlm.nih.gov/pubmed/24223144
http://dx.doi.org/10.1371/journal.pone.0078257
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author Hu, Jianzhong
Nomura, Yoko
Bashir, Ali
Fernandez-Hernandez, Heriberto
Itzkowitz, Steven
Pei, Zhiheng
Stone, Joanne
Loudon, Holly
Peter, Inga
author_facet Hu, Jianzhong
Nomura, Yoko
Bashir, Ali
Fernandez-Hernandez, Heriberto
Itzkowitz, Steven
Pei, Zhiheng
Stone, Joanne
Loudon, Holly
Peter, Inga
author_sort Hu, Jianzhong
collection PubMed
description OBJECTIVES: This study was aimed to assess the diversity of the meconium microbiome and determine if the bacterial community is affected by maternal diabetes status. METHODS: The first intestinal discharge (meconium) was collected from 23 newborns stratified by maternal diabetes status: 4 mothers had pre-gestational type 2 diabetes mellitus (DM) including one mother with dizygotic twins, 5 developed gestational diabetes mellitus (GDM) and 13 had no diabetes. The meconium microbiome was profiled using multi-barcode 16S rRNA sequencing followed by taxonomic assignment and diversity analysis. RESULTS: All meconium samples were not sterile and contained diversified microbiota. Compared with adult feces, the meconium showed a lower species diversity, higher sample-to-sample variation, and enrichment of Proteobacteria and reduction of Bacteroidetes. Among the meconium samples, the taxonomy analyses suggested that the overall bacterial content significantly differed by maternal diabetes status, with the microbiome of the DM group showing higher alpha-diversity than that of no-diabetes or GDM groups. No global difference was found between babies delivered vaginally versus via Cesarean-section. Regression analysis showed that the most robust predictor for the meconium microbiota composition was the maternal diabetes status that preceded pregnancy. Specifically, Bacteroidetes (phyla) and Parabacteriodes (genus) were enriched in the meconium in the DM group compared to the no-diabetes group. CONCLUSIONS: Our study provides evidence that meconium contains diversified microbiota and is not affected by the mode of delivery. It also suggests that the meconium microbiome of infants born to mothers with DM is enriched for the same bacterial taxa as those reported in the fecal microbiome of adult DM patients.
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spelling pubmed-38193832013-11-12 Diversified Microbiota of Meconium Is Affected by Maternal Diabetes Status Hu, Jianzhong Nomura, Yoko Bashir, Ali Fernandez-Hernandez, Heriberto Itzkowitz, Steven Pei, Zhiheng Stone, Joanne Loudon, Holly Peter, Inga PLoS One Research Article OBJECTIVES: This study was aimed to assess the diversity of the meconium microbiome and determine if the bacterial community is affected by maternal diabetes status. METHODS: The first intestinal discharge (meconium) was collected from 23 newborns stratified by maternal diabetes status: 4 mothers had pre-gestational type 2 diabetes mellitus (DM) including one mother with dizygotic twins, 5 developed gestational diabetes mellitus (GDM) and 13 had no diabetes. The meconium microbiome was profiled using multi-barcode 16S rRNA sequencing followed by taxonomic assignment and diversity analysis. RESULTS: All meconium samples were not sterile and contained diversified microbiota. Compared with adult feces, the meconium showed a lower species diversity, higher sample-to-sample variation, and enrichment of Proteobacteria and reduction of Bacteroidetes. Among the meconium samples, the taxonomy analyses suggested that the overall bacterial content significantly differed by maternal diabetes status, with the microbiome of the DM group showing higher alpha-diversity than that of no-diabetes or GDM groups. No global difference was found between babies delivered vaginally versus via Cesarean-section. Regression analysis showed that the most robust predictor for the meconium microbiota composition was the maternal diabetes status that preceded pregnancy. Specifically, Bacteroidetes (phyla) and Parabacteriodes (genus) were enriched in the meconium in the DM group compared to the no-diabetes group. CONCLUSIONS: Our study provides evidence that meconium contains diversified microbiota and is not affected by the mode of delivery. It also suggests that the meconium microbiome of infants born to mothers with DM is enriched for the same bacterial taxa as those reported in the fecal microbiome of adult DM patients. Public Library of Science 2013-11-06 /pmc/articles/PMC3819383/ /pubmed/24223144 http://dx.doi.org/10.1371/journal.pone.0078257 Text en © 2013 Hu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Hu, Jianzhong
Nomura, Yoko
Bashir, Ali
Fernandez-Hernandez, Heriberto
Itzkowitz, Steven
Pei, Zhiheng
Stone, Joanne
Loudon, Holly
Peter, Inga
Diversified Microbiota of Meconium Is Affected by Maternal Diabetes Status
title Diversified Microbiota of Meconium Is Affected by Maternal Diabetes Status
title_full Diversified Microbiota of Meconium Is Affected by Maternal Diabetes Status
title_fullStr Diversified Microbiota of Meconium Is Affected by Maternal Diabetes Status
title_full_unstemmed Diversified Microbiota of Meconium Is Affected by Maternal Diabetes Status
title_short Diversified Microbiota of Meconium Is Affected by Maternal Diabetes Status
title_sort diversified microbiota of meconium is affected by maternal diabetes status
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3819383/
https://www.ncbi.nlm.nih.gov/pubmed/24223144
http://dx.doi.org/10.1371/journal.pone.0078257
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