Evaluation of a commercial enzyme‐linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl

The ID Screen Influenza H5 Antibody Competition enzyme‐linked immunosorbent assay was tested for the detection of antibodies to the H5 subtype of influenza A (IA) virus in waterfowl. Assays were conducted with sera obtained from Mallards (Anas platyrhynchos) and Pekin Ducks (Anas platyrhynchos domes...

Descripción completa

Detalles Bibliográficos
Autores principales: Lebarbenchon, Camille, Pantin‐Jackwood, Mary, Kistler, Whitney M., Page Luttrell, M., Spackman, Erica, Stallknecht, David E., Brown, Justin D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2012
Materias:
Part 4
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3819717/
https://www.ncbi.nlm.nih.gov/pubmed/24192340
http://dx.doi.org/10.1111/irv.12070
_version_ 1782290028192333824
author Lebarbenchon, Camille
Pantin‐Jackwood, Mary
Kistler, Whitney M.
Page Luttrell, M.
Spackman, Erica
Stallknecht, David E.
Brown, Justin D.
author_facet Lebarbenchon, Camille
Pantin‐Jackwood, Mary
Kistler, Whitney M.
Page Luttrell, M.
Spackman, Erica
Stallknecht, David E.
Brown, Justin D.
author_sort Lebarbenchon, Camille
collection PubMed
description The ID Screen Influenza H5 Antibody Competition enzyme‐linked immunosorbent assay was tested for the detection of antibodies to the H5 subtype of influenza A (IA) virus in waterfowl. Assays were conducted with sera obtained from Mallards (Anas platyrhynchos) and Pekin Ducks (Anas platyrhynchos domestica), experimentally infected with eight low pathogenic (LP) and nine highly pathogenic (HP) H5N1 IA viral strains. Three incubation periods (1, 4 and 18 hours) and two dilutions (1:2 and 1:5) were tested. All serum samples from LP H5‐infected birds tested positive; however, improved detection rates were observed for viruses belonging to the HP H5N1 clade 2.2.1 as compared with those belonging to clade 2.1.3.
format Online
Article
Text
id pubmed-3819717
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-38197172014-11-01 Evaluation of a commercial enzyme‐linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl Lebarbenchon, Camille Pantin‐Jackwood, Mary Kistler, Whitney M. Page Luttrell, M. Spackman, Erica Stallknecht, David E. Brown, Justin D. Influenza Other Respir Viruses Part 4 The ID Screen Influenza H5 Antibody Competition enzyme‐linked immunosorbent assay was tested for the detection of antibodies to the H5 subtype of influenza A (IA) virus in waterfowl. Assays were conducted with sera obtained from Mallards (Anas platyrhynchos) and Pekin Ducks (Anas platyrhynchos domestica), experimentally infected with eight low pathogenic (LP) and nine highly pathogenic (HP) H5N1 IA viral strains. Three incubation periods (1, 4 and 18 hours) and two dilutions (1:2 and 1:5) were tested. All serum samples from LP H5‐infected birds tested positive; however, improved detection rates were observed for viruses belonging to the HP H5N1 clade 2.2.1 as compared with those belonging to clade 2.1.3. John Wiley and Sons Inc. 2012-12-22 2013-11 /pmc/articles/PMC3819717/ /pubmed/24192340 http://dx.doi.org/10.1111/irv.12070 Text en © 2012 John Wiley & Sons Ltd
spellingShingle Part 4
Lebarbenchon, Camille
Pantin‐Jackwood, Mary
Kistler, Whitney M.
Page Luttrell, M.
Spackman, Erica
Stallknecht, David E.
Brown, Justin D.
Evaluation of a commercial enzyme‐linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl
title Evaluation of a commercial enzyme‐linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl
title_full Evaluation of a commercial enzyme‐linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl
title_fullStr Evaluation of a commercial enzyme‐linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl
title_full_unstemmed Evaluation of a commercial enzyme‐linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl
title_short Evaluation of a commercial enzyme‐linked immunosorbent assay for detection of antibodies against the H5 subtype of Influenza A virus in waterfowl
title_sort evaluation of a commercial enzyme‐linked immunosorbent assay for detection of antibodies against the h5 subtype of influenza a virus in waterfowl
topic Part 4
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3819717/
https://www.ncbi.nlm.nih.gov/pubmed/24192340
http://dx.doi.org/10.1111/irv.12070
work_keys_str_mv AT lebarbenchoncamille evaluationofacommercialenzymelinkedimmunosorbentassayfordetectionofantibodiesagainsttheh5subtypeofinfluenzaavirusinwaterfowl
AT pantinjackwoodmary evaluationofacommercialenzymelinkedimmunosorbentassayfordetectionofantibodiesagainsttheh5subtypeofinfluenzaavirusinwaterfowl
AT kistlerwhitneym evaluationofacommercialenzymelinkedimmunosorbentassayfordetectionofantibodiesagainsttheh5subtypeofinfluenzaavirusinwaterfowl
AT pageluttrellm evaluationofacommercialenzymelinkedimmunosorbentassayfordetectionofantibodiesagainsttheh5subtypeofinfluenzaavirusinwaterfowl
AT spackmanerica evaluationofacommercialenzymelinkedimmunosorbentassayfordetectionofantibodiesagainsttheh5subtypeofinfluenzaavirusinwaterfowl
AT stallknechtdavide evaluationofacommercialenzymelinkedimmunosorbentassayfordetectionofantibodiesagainsttheh5subtypeofinfluenzaavirusinwaterfowl
AT brownjustind evaluationofacommercialenzymelinkedimmunosorbentassayfordetectionofantibodiesagainsttheh5subtypeofinfluenzaavirusinwaterfowl

Ejemplares similares