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Characterization of a Novel Enterovirus Serotype and an Enterovirus EV-B93 Isolated from Acute Flaccid Paralysis Patients

Non-polio enteroviruses (NPEVs) are among the most common viruses infecting humans worldwide. Most of these infections are asymptomatic but few can lead to systemic and neurological disorders like Acute Flaccid Paralysis (AFP). Acute Flaccid Paralysis is a clinical syndrome and NPEVs have been isola...

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Autores principales: Shaukat, Shahzad, Angez, Mehar, Alam, Muhammad Masroor, Sharif, Salmaan, Khurshid, Adnan, Mahmood, Tariq, Zaidi, Syed Sohail Zahoor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3820551/
https://www.ncbi.nlm.nih.gov/pubmed/24244603
http://dx.doi.org/10.1371/journal.pone.0080040
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author Shaukat, Shahzad
Angez, Mehar
Alam, Muhammad Masroor
Sharif, Salmaan
Khurshid, Adnan
Mahmood, Tariq
Zaidi, Syed Sohail Zahoor
author_facet Shaukat, Shahzad
Angez, Mehar
Alam, Muhammad Masroor
Sharif, Salmaan
Khurshid, Adnan
Mahmood, Tariq
Zaidi, Syed Sohail Zahoor
author_sort Shaukat, Shahzad
collection PubMed
description Non-polio enteroviruses (NPEVs) are among the most common viruses infecting humans worldwide. Most of these infections are asymptomatic but few can lead to systemic and neurological disorders like Acute Flaccid Paralysis (AFP). Acute Flaccid Paralysis is a clinical syndrome and NPEVs have been isolated frequently from the patients suffering from AFP but little is known about their causal relationship. The objective of this study was to identify and characterize the NPEV serotypes recovered from 184 stool samples collected from AFP patients in Federally Administered Tribal Areas (FATA) in north-west of Pakistan. Overall, 44 (95.6 %) isolates were successfully typed through microneutralization assay as a member of enterovirus B species including echovirus (E)-2, E-3, E-4, E-6, E-7, E-11, E-13, E-14, E-21 and E-29 while two isolates (PAK NIH SP6545B and PAK NIH SP1202B) remained untypeable. The VP1 and capsid regions analysis characterized these viruses as EV-B93 and EV-B106. Phylogenetic analysis confirmed that PAK NIH isolates had high genetic diversity and represent distinct genotypes circulating in the country. Our findings highlight the role of NPEVs in AFP cases to be thoroughly investigated especially in high disease risk areas, with limited surveillance activities and health resources.
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spelling pubmed-38205512013-11-15 Characterization of a Novel Enterovirus Serotype and an Enterovirus EV-B93 Isolated from Acute Flaccid Paralysis Patients Shaukat, Shahzad Angez, Mehar Alam, Muhammad Masroor Sharif, Salmaan Khurshid, Adnan Mahmood, Tariq Zaidi, Syed Sohail Zahoor PLoS One Research Article Non-polio enteroviruses (NPEVs) are among the most common viruses infecting humans worldwide. Most of these infections are asymptomatic but few can lead to systemic and neurological disorders like Acute Flaccid Paralysis (AFP). Acute Flaccid Paralysis is a clinical syndrome and NPEVs have been isolated frequently from the patients suffering from AFP but little is known about their causal relationship. The objective of this study was to identify and characterize the NPEV serotypes recovered from 184 stool samples collected from AFP patients in Federally Administered Tribal Areas (FATA) in north-west of Pakistan. Overall, 44 (95.6 %) isolates were successfully typed through microneutralization assay as a member of enterovirus B species including echovirus (E)-2, E-3, E-4, E-6, E-7, E-11, E-13, E-14, E-21 and E-29 while two isolates (PAK NIH SP6545B and PAK NIH SP1202B) remained untypeable. The VP1 and capsid regions analysis characterized these viruses as EV-B93 and EV-B106. Phylogenetic analysis confirmed that PAK NIH isolates had high genetic diversity and represent distinct genotypes circulating in the country. Our findings highlight the role of NPEVs in AFP cases to be thoroughly investigated especially in high disease risk areas, with limited surveillance activities and health resources. Public Library of Science 2013-11-07 /pmc/articles/PMC3820551/ /pubmed/24244603 http://dx.doi.org/10.1371/journal.pone.0080040 Text en © 2013 Shaukat et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Shaukat, Shahzad
Angez, Mehar
Alam, Muhammad Masroor
Sharif, Salmaan
Khurshid, Adnan
Mahmood, Tariq
Zaidi, Syed Sohail Zahoor
Characterization of a Novel Enterovirus Serotype and an Enterovirus EV-B93 Isolated from Acute Flaccid Paralysis Patients
title Characterization of a Novel Enterovirus Serotype and an Enterovirus EV-B93 Isolated from Acute Flaccid Paralysis Patients
title_full Characterization of a Novel Enterovirus Serotype and an Enterovirus EV-B93 Isolated from Acute Flaccid Paralysis Patients
title_fullStr Characterization of a Novel Enterovirus Serotype and an Enterovirus EV-B93 Isolated from Acute Flaccid Paralysis Patients
title_full_unstemmed Characterization of a Novel Enterovirus Serotype and an Enterovirus EV-B93 Isolated from Acute Flaccid Paralysis Patients
title_short Characterization of a Novel Enterovirus Serotype and an Enterovirus EV-B93 Isolated from Acute Flaccid Paralysis Patients
title_sort characterization of a novel enterovirus serotype and an enterovirus ev-b93 isolated from acute flaccid paralysis patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3820551/
https://www.ncbi.nlm.nih.gov/pubmed/24244603
http://dx.doi.org/10.1371/journal.pone.0080040
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