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Inhibition of fibrosis and inflammation by triple therapy with pirfenidone, edaravone and erythropoietin in rabbits with drug-induced lung injury: comparison of CT imaging and pathological findings
In a rabbit model of bleomycin-induced lung injury, computed tomography (CT) and pathological studies were conducted to investigate whether the progression of this injury is inhibited by pirfenidone and by triple therapy with pirfenidone, edaravone and erythropoietin. We divided nine rabbits with bl...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3820844/ https://www.ncbi.nlm.nih.gov/pubmed/24223628 http://dx.doi.org/10.3892/etm.2013.1308 |
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author | WATANABE, SHOBU NITTA, NORIHISA SONODA, AKINAGA NITTA-SEKO, AYUMI OHTA, SHINICHI TSUCHIYA, KEIKO OTANI, HIDEJI TOMOZAWA, YUKI NAGATANI, YUKIHIRO MUKAISHO, KENICHI TAKAHASHI, MASASHI MURATA, KIYOSHI |
author_facet | WATANABE, SHOBU NITTA, NORIHISA SONODA, AKINAGA NITTA-SEKO, AYUMI OHTA, SHINICHI TSUCHIYA, KEIKO OTANI, HIDEJI TOMOZAWA, YUKI NAGATANI, YUKIHIRO MUKAISHO, KENICHI TAKAHASHI, MASASHI MURATA, KIYOSHI |
author_sort | WATANABE, SHOBU |
collection | PubMed |
description | In a rabbit model of bleomycin-induced lung injury, computed tomography (CT) and pathological studies were conducted to investigate whether the progression of this injury is inhibited by pirfenidone and by triple therapy with pirfenidone, edaravone and erythropoietin. We divided nine rabbits with bleomycin-induced lung injury into three equally sized groups. Group 1 served as the control, group 2 received pirfenidone alone and group 3 was treated with pirfenidone, edaravone and erythropoietin. Multidetector CT (MDCT) scans were acquired immediately after the administration of bleomycin, and further scans were performed on days 14 and 28. The area of abnormal opacity was calculated. The rabbit lungs were removed and the size of abnormal areas in macroscopic specimens was calculated and the degree of fibrosis and inflammation in microscopic specimens was scored. In order, the average size of the area of abnormal opacity on CT scans was largest in group 1, followed by groups 2 and 3. On day 28, the area of opacity was significantly smaller in group 3 than in group 1 (P=0.071). The average size of the area of abnormal opacity on macroscopic findings was largest in group 1, followed in order by groups 2 and 3; the difference between group 1 and 3 was significant (P<0.05). The average fibrosis score was highest in group 3 followed by groups 2 and 1. By contrast, the average inflammation score was highest in group 2 followed by groups 1 and 3. Although the administration of pirfenidone alone slowed the progression of bleomycin-induced lung injury, the triple-drug combination was more effective. |
format | Online Article Text |
id | pubmed-3820844 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-38208442013-11-09 Inhibition of fibrosis and inflammation by triple therapy with pirfenidone, edaravone and erythropoietin in rabbits with drug-induced lung injury: comparison of CT imaging and pathological findings WATANABE, SHOBU NITTA, NORIHISA SONODA, AKINAGA NITTA-SEKO, AYUMI OHTA, SHINICHI TSUCHIYA, KEIKO OTANI, HIDEJI TOMOZAWA, YUKI NAGATANI, YUKIHIRO MUKAISHO, KENICHI TAKAHASHI, MASASHI MURATA, KIYOSHI Exp Ther Med Articles In a rabbit model of bleomycin-induced lung injury, computed tomography (CT) and pathological studies were conducted to investigate whether the progression of this injury is inhibited by pirfenidone and by triple therapy with pirfenidone, edaravone and erythropoietin. We divided nine rabbits with bleomycin-induced lung injury into three equally sized groups. Group 1 served as the control, group 2 received pirfenidone alone and group 3 was treated with pirfenidone, edaravone and erythropoietin. Multidetector CT (MDCT) scans were acquired immediately after the administration of bleomycin, and further scans were performed on days 14 and 28. The area of abnormal opacity was calculated. The rabbit lungs were removed and the size of abnormal areas in macroscopic specimens was calculated and the degree of fibrosis and inflammation in microscopic specimens was scored. In order, the average size of the area of abnormal opacity on CT scans was largest in group 1, followed by groups 2 and 3. On day 28, the area of opacity was significantly smaller in group 3 than in group 1 (P=0.071). The average size of the area of abnormal opacity on macroscopic findings was largest in group 1, followed in order by groups 2 and 3; the difference between group 1 and 3 was significant (P<0.05). The average fibrosis score was highest in group 3 followed by groups 2 and 1. By contrast, the average inflammation score was highest in group 2 followed by groups 1 and 3. Although the administration of pirfenidone alone slowed the progression of bleomycin-induced lung injury, the triple-drug combination was more effective. D.A. Spandidos 2013-11 2013-09-18 /pmc/articles/PMC3820844/ /pubmed/24223628 http://dx.doi.org/10.3892/etm.2013.1308 Text en Copyright © 2013, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles WATANABE, SHOBU NITTA, NORIHISA SONODA, AKINAGA NITTA-SEKO, AYUMI OHTA, SHINICHI TSUCHIYA, KEIKO OTANI, HIDEJI TOMOZAWA, YUKI NAGATANI, YUKIHIRO MUKAISHO, KENICHI TAKAHASHI, MASASHI MURATA, KIYOSHI Inhibition of fibrosis and inflammation by triple therapy with pirfenidone, edaravone and erythropoietin in rabbits with drug-induced lung injury: comparison of CT imaging and pathological findings |
title | Inhibition of fibrosis and inflammation by triple therapy with pirfenidone, edaravone and erythropoietin in rabbits with drug-induced lung injury: comparison of CT imaging and pathological findings |
title_full | Inhibition of fibrosis and inflammation by triple therapy with pirfenidone, edaravone and erythropoietin in rabbits with drug-induced lung injury: comparison of CT imaging and pathological findings |
title_fullStr | Inhibition of fibrosis and inflammation by triple therapy with pirfenidone, edaravone and erythropoietin in rabbits with drug-induced lung injury: comparison of CT imaging and pathological findings |
title_full_unstemmed | Inhibition of fibrosis and inflammation by triple therapy with pirfenidone, edaravone and erythropoietin in rabbits with drug-induced lung injury: comparison of CT imaging and pathological findings |
title_short | Inhibition of fibrosis and inflammation by triple therapy with pirfenidone, edaravone and erythropoietin in rabbits with drug-induced lung injury: comparison of CT imaging and pathological findings |
title_sort | inhibition of fibrosis and inflammation by triple therapy with pirfenidone, edaravone and erythropoietin in rabbits with drug-induced lung injury: comparison of ct imaging and pathological findings |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3820844/ https://www.ncbi.nlm.nih.gov/pubmed/24223628 http://dx.doi.org/10.3892/etm.2013.1308 |
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