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Investigations on Aberrant Glycosylation of Glycosphingolipids in Colorectal Cancer Tissues Using Liquid Chromatography and Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry (MALDI-TOF-MS)

Cancer is a leading cause of death and alterations of glycosylation are characteristic features of malignant cells. Colorectal cancer is one of the most common cancers and its exact causes and biology are not yet well understood. Here, we compared glycosylation profiles of colorectal tumor tissues a...

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Autores principales: Holst, Stephanie, Stavenhagen, Kathrin, Balog, Crina I. A., Koeleman, Carolien A. M., McDonnell, Liam M., Mayboroda, Oleg A., Verhoeven, Aswin, Mesker, Wilma E., Tollenaar, Rob A. E. M., Deelder, André M., Wuhrer, Manfred
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Biochemistry and Molecular Biology 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3820925/
https://www.ncbi.nlm.nih.gov/pubmed/23878401
http://dx.doi.org/10.1074/mcp.M113.030387
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author Holst, Stephanie
Stavenhagen, Kathrin
Balog, Crina I. A.
Koeleman, Carolien A. M.
McDonnell, Liam M.
Mayboroda, Oleg A.
Verhoeven, Aswin
Mesker, Wilma E.
Tollenaar, Rob A. E. M.
Deelder, André M.
Wuhrer, Manfred
author_facet Holst, Stephanie
Stavenhagen, Kathrin
Balog, Crina I. A.
Koeleman, Carolien A. M.
McDonnell, Liam M.
Mayboroda, Oleg A.
Verhoeven, Aswin
Mesker, Wilma E.
Tollenaar, Rob A. E. M.
Deelder, André M.
Wuhrer, Manfred
author_sort Holst, Stephanie
collection PubMed
description Cancer is a leading cause of death and alterations of glycosylation are characteristic features of malignant cells. Colorectal cancer is one of the most common cancers and its exact causes and biology are not yet well understood. Here, we compared glycosylation profiles of colorectal tumor tissues and corresponding control tissues of 13 colorectal cancer patients to contribute to the understanding of this cancer. Using MALDI-TOF(/TOF)-MS and 2-dimensional LC-MS/MS we characterized enzymatically released and 2-aminobenzoic acid labeled glycans from glycosphingolipids. Multivariate data analysis revealed significant differences between tumor and corresponding control tissues. Main discriminators were obtained, which represent the overall alteration in glycosylation of glycosphingolipids during colorectal cancer progression, and these were found to be characterized by (1) increased fucosylation, (2) decreased acetylation, (3) decreased sulfation, (4) reduced expression of globo-type glycans, as well as (5) disialyl gangliosides. The findings of our current research confirm former reports, and in addition expand the knowledge of glycosphingolipid glycosylation in colorectal cancer by revealing new glycans with discriminative power and characteristic, cancer-associated glycosylation alterations. The obtained discriminating glycans can contribute to progress the discovery of biomarkers to improve diagnostics and patient treatment.
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spelling pubmed-38209252013-11-15 Investigations on Aberrant Glycosylation of Glycosphingolipids in Colorectal Cancer Tissues Using Liquid Chromatography and Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) Holst, Stephanie Stavenhagen, Kathrin Balog, Crina I. A. Koeleman, Carolien A. M. McDonnell, Liam M. Mayboroda, Oleg A. Verhoeven, Aswin Mesker, Wilma E. Tollenaar, Rob A. E. M. Deelder, André M. Wuhrer, Manfred Mol Cell Proteomics Research Cancer is a leading cause of death and alterations of glycosylation are characteristic features of malignant cells. Colorectal cancer is one of the most common cancers and its exact causes and biology are not yet well understood. Here, we compared glycosylation profiles of colorectal tumor tissues and corresponding control tissues of 13 colorectal cancer patients to contribute to the understanding of this cancer. Using MALDI-TOF(/TOF)-MS and 2-dimensional LC-MS/MS we characterized enzymatically released and 2-aminobenzoic acid labeled glycans from glycosphingolipids. Multivariate data analysis revealed significant differences between tumor and corresponding control tissues. Main discriminators were obtained, which represent the overall alteration in glycosylation of glycosphingolipids during colorectal cancer progression, and these were found to be characterized by (1) increased fucosylation, (2) decreased acetylation, (3) decreased sulfation, (4) reduced expression of globo-type glycans, as well as (5) disialyl gangliosides. The findings of our current research confirm former reports, and in addition expand the knowledge of glycosphingolipid glycosylation in colorectal cancer by revealing new glycans with discriminative power and characteristic, cancer-associated glycosylation alterations. The obtained discriminating glycans can contribute to progress the discovery of biomarkers to improve diagnostics and patient treatment. The American Society for Biochemistry and Molecular Biology 2013-11 2013-07-22 /pmc/articles/PMC3820925/ /pubmed/23878401 http://dx.doi.org/10.1074/mcp.M113.030387 Text en © 2013 by The American Society for Biochemistry and Molecular Biology, Inc. Author's Choice—Final version full access.
spellingShingle Research
Holst, Stephanie
Stavenhagen, Kathrin
Balog, Crina I. A.
Koeleman, Carolien A. M.
McDonnell, Liam M.
Mayboroda, Oleg A.
Verhoeven, Aswin
Mesker, Wilma E.
Tollenaar, Rob A. E. M.
Deelder, André M.
Wuhrer, Manfred
Investigations on Aberrant Glycosylation of Glycosphingolipids in Colorectal Cancer Tissues Using Liquid Chromatography and Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry (MALDI-TOF-MS)
title Investigations on Aberrant Glycosylation of Glycosphingolipids in Colorectal Cancer Tissues Using Liquid Chromatography and Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry (MALDI-TOF-MS)
title_full Investigations on Aberrant Glycosylation of Glycosphingolipids in Colorectal Cancer Tissues Using Liquid Chromatography and Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry (MALDI-TOF-MS)
title_fullStr Investigations on Aberrant Glycosylation of Glycosphingolipids in Colorectal Cancer Tissues Using Liquid Chromatography and Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry (MALDI-TOF-MS)
title_full_unstemmed Investigations on Aberrant Glycosylation of Glycosphingolipids in Colorectal Cancer Tissues Using Liquid Chromatography and Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry (MALDI-TOF-MS)
title_short Investigations on Aberrant Glycosylation of Glycosphingolipids in Colorectal Cancer Tissues Using Liquid Chromatography and Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry (MALDI-TOF-MS)
title_sort investigations on aberrant glycosylation of glycosphingolipids in colorectal cancer tissues using liquid chromatography and matrix-assisted laser desorption time-of-flight mass spectrometry (maldi-tof-ms)
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3820925/
https://www.ncbi.nlm.nih.gov/pubmed/23878401
http://dx.doi.org/10.1074/mcp.M113.030387
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