Murine homologue of the human KIAA1199 is implicated in hyaluronan binding and depolymerization()

Recently, we have disclosed that human KIAA1199 (hKIAA1199) is a hyaluronan (HA) binding protein implicated in HA depolymerization. Although a murine homologue (mKiaa1199) was previously cloned, no information about the function of the molecule was available. Here, we show that cells transfected wit...

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Autores principales: Yoshida, Hiroyuki, Nagaoka, Aya, Nakamura, Sachiko, Sugiyama, Yoshinori, Okada, Yasunori, Inoue, Shintaro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3821019/
https://www.ncbi.nlm.nih.gov/pubmed/24251095
http://dx.doi.org/10.1016/j.fob.2013.08.003
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author Yoshida, Hiroyuki
Nagaoka, Aya
Nakamura, Sachiko
Sugiyama, Yoshinori
Okada, Yasunori
Inoue, Shintaro
author_facet Yoshida, Hiroyuki
Nagaoka, Aya
Nakamura, Sachiko
Sugiyama, Yoshinori
Okada, Yasunori
Inoue, Shintaro
author_sort Yoshida, Hiroyuki
collection PubMed
description Recently, we have disclosed that human KIAA1199 (hKIAA1199) is a hyaluronan (HA) binding protein implicated in HA depolymerization. Although a murine homologue (mKiaa1199) was previously cloned, no information about the function of the molecule was available. Here, we show that cells transfected with mKiaa1199 cDNA selectively catabolized HA via the clathrin-coated pit pathway. A glycosaminoglycan-binding assay demonstrated the specific binding of mKiaa1199 to HA. These results were similar to our observations with hKIAA1199, although slight differences were found in the peak sizes of the minimum degradates of HA. We conclude that like hKIAA1199, mKiaa1199 is a hyaladherin, leading to HA depolymerization.
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spelling pubmed-38210192013-11-18 Murine homologue of the human KIAA1199 is implicated in hyaluronan binding and depolymerization() Yoshida, Hiroyuki Nagaoka, Aya Nakamura, Sachiko Sugiyama, Yoshinori Okada, Yasunori Inoue, Shintaro FEBS Open Bio Article Recently, we have disclosed that human KIAA1199 (hKIAA1199) is a hyaluronan (HA) binding protein implicated in HA depolymerization. Although a murine homologue (mKiaa1199) was previously cloned, no information about the function of the molecule was available. Here, we show that cells transfected with mKiaa1199 cDNA selectively catabolized HA via the clathrin-coated pit pathway. A glycosaminoglycan-binding assay demonstrated the specific binding of mKiaa1199 to HA. These results were similar to our observations with hKIAA1199, although slight differences were found in the peak sizes of the minimum degradates of HA. We conclude that like hKIAA1199, mKiaa1199 is a hyaladherin, leading to HA depolymerization. Elsevier 2013-08-17 /pmc/articles/PMC3821019/ /pubmed/24251095 http://dx.doi.org/10.1016/j.fob.2013.08.003 Text en © 2013 The Authors http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License, which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Yoshida, Hiroyuki
Nagaoka, Aya
Nakamura, Sachiko
Sugiyama, Yoshinori
Okada, Yasunori
Inoue, Shintaro
Murine homologue of the human KIAA1199 is implicated in hyaluronan binding and depolymerization()
title Murine homologue of the human KIAA1199 is implicated in hyaluronan binding and depolymerization()
title_full Murine homologue of the human KIAA1199 is implicated in hyaluronan binding and depolymerization()
title_fullStr Murine homologue of the human KIAA1199 is implicated in hyaluronan binding and depolymerization()
title_full_unstemmed Murine homologue of the human KIAA1199 is implicated in hyaluronan binding and depolymerization()
title_short Murine homologue of the human KIAA1199 is implicated in hyaluronan binding and depolymerization()
title_sort murine homologue of the human kiaa1199 is implicated in hyaluronan binding and depolymerization()
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3821019/
https://www.ncbi.nlm.nih.gov/pubmed/24251095
http://dx.doi.org/10.1016/j.fob.2013.08.003
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