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Significant Overexpression of DVL1 in Taiwanese Colorectal Cancer Patients with Liver Metastasis

Undetected micrometastasis plays a key role in the metastasis of cancer in colorectal cancer (CRC) patients. The aim of this study is to identify a biomarker of CRC patients with liver metastasis through the detection of circulating tumor cells (CTCs). Microarray and bioinformatics analysis of 10 CR...

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Autores principales: Huang, Ming-Yii, Yen, Li-Chen, Liu, Hsueh-Chiao, Liu, Po-Ping, Chung, Fu-Yen, Wang, Tsu-Nai, Wang, Jaw-Yuan, Lin, Shiu-Ru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3821627/
https://www.ncbi.nlm.nih.gov/pubmed/24129181
http://dx.doi.org/10.3390/ijms141020492
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author Huang, Ming-Yii
Yen, Li-Chen
Liu, Hsueh-Chiao
Liu, Po-Ping
Chung, Fu-Yen
Wang, Tsu-Nai
Wang, Jaw-Yuan
Lin, Shiu-Ru
author_facet Huang, Ming-Yii
Yen, Li-Chen
Liu, Hsueh-Chiao
Liu, Po-Ping
Chung, Fu-Yen
Wang, Tsu-Nai
Wang, Jaw-Yuan
Lin, Shiu-Ru
author_sort Huang, Ming-Yii
collection PubMed
description Undetected micrometastasis plays a key role in the metastasis of cancer in colorectal cancer (CRC) patients. The aim of this study is to identify a biomarker of CRC patients with liver metastasis through the detection of circulating tumor cells (CTCs). Microarray and bioinformatics analysis of 10 CRC cancer tissue specimens compared with normal adjacent tissues revealed that 31 genes were up-regulated (gene expression ratio of cancer tissue to paired normal tissue > 2) in the cancer patients. We used a weighted enzymatic chip array (WEnCA) including 31 prognosis-related genes to investigate CTCs in 214 postoperative stage I–III CRC patients and to analyze the correlation between gene expression and clinico-pathological parameters. We employed the immunohistochemistry (IHC) method with polyclonal mouse antibody against DVL1 to detect DVL1 expression in 60 CRC patients. CRC liver metastasis occurred in 19.16% (41/214) of the patients. Using univariate analysis and multivariate proportional hazards regression analysis, we found that DVL1 mRNA overexpression had a significant, independent predictive value for liver metastasis in CRC patients (OR: 5.764; 95% CI: 2.588–12.837; p < 0.0001 on univariate analysis; OR: 3.768; 95% CI: 1.469–9.665; p = 0.006 on multivariate analysis). IHC staining of the immunoreactivity of DVL1 showed that DVL1 was localized in the cytoplasm of CRC cells. High expression of DVL1 was observed in 55% (33/60) of CRC tumor specimens and was associated significantly with tumor depth, perineural invasion and liver metastasis status (all p < 0.05). Our experimental results demonstrated that DVL1 is significantly overexpressed in CRC patients with liver metastasis, leading us to conclude that DVL1 could be a potential prognostic and predictive marker for CRC patients.
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spelling pubmed-38216272013-11-11 Significant Overexpression of DVL1 in Taiwanese Colorectal Cancer Patients with Liver Metastasis Huang, Ming-Yii Yen, Li-Chen Liu, Hsueh-Chiao Liu, Po-Ping Chung, Fu-Yen Wang, Tsu-Nai Wang, Jaw-Yuan Lin, Shiu-Ru Int J Mol Sci Article Undetected micrometastasis plays a key role in the metastasis of cancer in colorectal cancer (CRC) patients. The aim of this study is to identify a biomarker of CRC patients with liver metastasis through the detection of circulating tumor cells (CTCs). Microarray and bioinformatics analysis of 10 CRC cancer tissue specimens compared with normal adjacent tissues revealed that 31 genes were up-regulated (gene expression ratio of cancer tissue to paired normal tissue > 2) in the cancer patients. We used a weighted enzymatic chip array (WEnCA) including 31 prognosis-related genes to investigate CTCs in 214 postoperative stage I–III CRC patients and to analyze the correlation between gene expression and clinico-pathological parameters. We employed the immunohistochemistry (IHC) method with polyclonal mouse antibody against DVL1 to detect DVL1 expression in 60 CRC patients. CRC liver metastasis occurred in 19.16% (41/214) of the patients. Using univariate analysis and multivariate proportional hazards regression analysis, we found that DVL1 mRNA overexpression had a significant, independent predictive value for liver metastasis in CRC patients (OR: 5.764; 95% CI: 2.588–12.837; p < 0.0001 on univariate analysis; OR: 3.768; 95% CI: 1.469–9.665; p = 0.006 on multivariate analysis). IHC staining of the immunoreactivity of DVL1 showed that DVL1 was localized in the cytoplasm of CRC cells. High expression of DVL1 was observed in 55% (33/60) of CRC tumor specimens and was associated significantly with tumor depth, perineural invasion and liver metastasis status (all p < 0.05). Our experimental results demonstrated that DVL1 is significantly overexpressed in CRC patients with liver metastasis, leading us to conclude that DVL1 could be a potential prognostic and predictive marker for CRC patients. Molecular Diversity Preservation International (MDPI) 2013-10-14 /pmc/articles/PMC3821627/ /pubmed/24129181 http://dx.doi.org/10.3390/ijms141020492 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Huang, Ming-Yii
Yen, Li-Chen
Liu, Hsueh-Chiao
Liu, Po-Ping
Chung, Fu-Yen
Wang, Tsu-Nai
Wang, Jaw-Yuan
Lin, Shiu-Ru
Significant Overexpression of DVL1 in Taiwanese Colorectal Cancer Patients with Liver Metastasis
title Significant Overexpression of DVL1 in Taiwanese Colorectal Cancer Patients with Liver Metastasis
title_full Significant Overexpression of DVL1 in Taiwanese Colorectal Cancer Patients with Liver Metastasis
title_fullStr Significant Overexpression of DVL1 in Taiwanese Colorectal Cancer Patients with Liver Metastasis
title_full_unstemmed Significant Overexpression of DVL1 in Taiwanese Colorectal Cancer Patients with Liver Metastasis
title_short Significant Overexpression of DVL1 in Taiwanese Colorectal Cancer Patients with Liver Metastasis
title_sort significant overexpression of dvl1 in taiwanese colorectal cancer patients with liver metastasis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3821627/
https://www.ncbi.nlm.nih.gov/pubmed/24129181
http://dx.doi.org/10.3390/ijms141020492
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