Cargando…
Combined Taurine, Epigallocatechin Gallate and Genistein Therapy Reduces HSC-T6 Cell Proliferation and Modulates the Expression of Fibrogenic Factors
Hepatic fibrogenesis involves the activation of hepatic stellate cells (HSCs), which synthesize excess extracellular matrix and contribute to the development of liver fibrosis. In a prior study we tested the effect of combined treatment with taurine, epigallocatechin gallate and genistein on the dev...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3821629/ https://www.ncbi.nlm.nih.gov/pubmed/24129183 http://dx.doi.org/10.3390/ijms141020543 |
_version_ | 1782290331565293568 |
---|---|
author | Li, Yan Luo, Ying Zhang, Xuerong Lin, Xing He, Min Liao, Ming |
author_facet | Li, Yan Luo, Ying Zhang, Xuerong Lin, Xing He, Min Liao, Ming |
author_sort | Li, Yan |
collection | PubMed |
description | Hepatic fibrogenesis involves the activation of hepatic stellate cells (HSCs), which synthesize excess extracellular matrix and contribute to the development of liver fibrosis. In a prior study we tested the effect of combined treatment with taurine, epigallocatechin gallate and genistein on the development of alcohol-induced liver fibrosis in vitro. In this study, the biological activity of the combination of these molecules was assessed by measuring its effect on cell proliferation, fibrosis-related gene expression, and proteomic expression profiling in the activated HSC cell line, HSC-T6. HSC-T6 cells were incubated with different concentrations of the drug combination taurine, epigallocatechin gallate and genistein. Cell proliferation was evaluated by MTT assay. Transforming growth factor β1 (TGF-β1), collagen type I (Col-I), matrix metalloproteinase-2 (MMP-2), and tissue inhibitor of metalloproteinases 1 and 2 (TIMP-1 and TIMP-2) mRNA were analyzed by semi-quantitative reverse-transcription PCR. Proteomic profiling of HSC-T6 cells was also performed by SELDI-TOF-MS. Combined drug treatment significantly inhibited cell proliferation and TGF-β1, Col-I, TIMP-1 and TIMP-2 mRNA expression in activated HSC-T6 cells, while the expression of MMP-2 mRNA increased. A total of 176 protein m/z peaks were identified. The intensities of 10 protein peaks were downregulated and two protein peaks were upregulated in HSC-T6 cells after combined drug treatment. In conclusion, combined drug treatment with taurine, epigallocatechin gallate and genistein can inhibit HSC proliferation, and impact fibrosis-related gene and protein expression. The antifibrotic effects of this drug combination may be due to its effects on the expression of fibrogenic genes. |
format | Online Article Text |
id | pubmed-3821629 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-38216292013-11-11 Combined Taurine, Epigallocatechin Gallate and Genistein Therapy Reduces HSC-T6 Cell Proliferation and Modulates the Expression of Fibrogenic Factors Li, Yan Luo, Ying Zhang, Xuerong Lin, Xing He, Min Liao, Ming Int J Mol Sci Article Hepatic fibrogenesis involves the activation of hepatic stellate cells (HSCs), which synthesize excess extracellular matrix and contribute to the development of liver fibrosis. In a prior study we tested the effect of combined treatment with taurine, epigallocatechin gallate and genistein on the development of alcohol-induced liver fibrosis in vitro. In this study, the biological activity of the combination of these molecules was assessed by measuring its effect on cell proliferation, fibrosis-related gene expression, and proteomic expression profiling in the activated HSC cell line, HSC-T6. HSC-T6 cells were incubated with different concentrations of the drug combination taurine, epigallocatechin gallate and genistein. Cell proliferation was evaluated by MTT assay. Transforming growth factor β1 (TGF-β1), collagen type I (Col-I), matrix metalloproteinase-2 (MMP-2), and tissue inhibitor of metalloproteinases 1 and 2 (TIMP-1 and TIMP-2) mRNA were analyzed by semi-quantitative reverse-transcription PCR. Proteomic profiling of HSC-T6 cells was also performed by SELDI-TOF-MS. Combined drug treatment significantly inhibited cell proliferation and TGF-β1, Col-I, TIMP-1 and TIMP-2 mRNA expression in activated HSC-T6 cells, while the expression of MMP-2 mRNA increased. A total of 176 protein m/z peaks were identified. The intensities of 10 protein peaks were downregulated and two protein peaks were upregulated in HSC-T6 cells after combined drug treatment. In conclusion, combined drug treatment with taurine, epigallocatechin gallate and genistein can inhibit HSC proliferation, and impact fibrosis-related gene and protein expression. The antifibrotic effects of this drug combination may be due to its effects on the expression of fibrogenic genes. Molecular Diversity Preservation International (MDPI) 2013-10-14 /pmc/articles/PMC3821629/ /pubmed/24129183 http://dx.doi.org/10.3390/ijms141020543 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Article Li, Yan Luo, Ying Zhang, Xuerong Lin, Xing He, Min Liao, Ming Combined Taurine, Epigallocatechin Gallate and Genistein Therapy Reduces HSC-T6 Cell Proliferation and Modulates the Expression of Fibrogenic Factors |
title | Combined Taurine, Epigallocatechin Gallate and Genistein Therapy Reduces HSC-T6 Cell Proliferation and Modulates the Expression of Fibrogenic Factors |
title_full | Combined Taurine, Epigallocatechin Gallate and Genistein Therapy Reduces HSC-T6 Cell Proliferation and Modulates the Expression of Fibrogenic Factors |
title_fullStr | Combined Taurine, Epigallocatechin Gallate and Genistein Therapy Reduces HSC-T6 Cell Proliferation and Modulates the Expression of Fibrogenic Factors |
title_full_unstemmed | Combined Taurine, Epigallocatechin Gallate and Genistein Therapy Reduces HSC-T6 Cell Proliferation and Modulates the Expression of Fibrogenic Factors |
title_short | Combined Taurine, Epigallocatechin Gallate and Genistein Therapy Reduces HSC-T6 Cell Proliferation and Modulates the Expression of Fibrogenic Factors |
title_sort | combined taurine, epigallocatechin gallate and genistein therapy reduces hsc-t6 cell proliferation and modulates the expression of fibrogenic factors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3821629/ https://www.ncbi.nlm.nih.gov/pubmed/24129183 http://dx.doi.org/10.3390/ijms141020543 |
work_keys_str_mv | AT liyan combinedtaurineepigallocatechingallateandgenisteintherapyreduceshsct6cellproliferationandmodulatestheexpressionoffibrogenicfactors AT luoying combinedtaurineepigallocatechingallateandgenisteintherapyreduceshsct6cellproliferationandmodulatestheexpressionoffibrogenicfactors AT zhangxuerong combinedtaurineepigallocatechingallateandgenisteintherapyreduceshsct6cellproliferationandmodulatestheexpressionoffibrogenicfactors AT linxing combinedtaurineepigallocatechingallateandgenisteintherapyreduceshsct6cellproliferationandmodulatestheexpressionoffibrogenicfactors AT hemin combinedtaurineepigallocatechingallateandgenisteintherapyreduceshsct6cellproliferationandmodulatestheexpressionoffibrogenicfactors AT liaoming combinedtaurineepigallocatechingallateandgenisteintherapyreduceshsct6cellproliferationandmodulatestheexpressionoffibrogenicfactors |