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The Effect on Proliferation and Differentiation of Cementoblast by Using Sclerostin as Inhibitor

Cementogenesis is of great importance for normal teeth root development and is involved in the repair process of root resorption caused by orthodontic treatment. As highly differentiated mesenchymal cells, cementoblasts are responsible for this process under the regulation of many endogenous agents....

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Autores principales: Bao, Xingfu, Liu, Yuyan, Han, Guanghong, Zuo, Zhigang, Hu, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3821662/
https://www.ncbi.nlm.nih.gov/pubmed/24152444
http://dx.doi.org/10.3390/ijms141021140
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author Bao, Xingfu
Liu, Yuyan
Han, Guanghong
Zuo, Zhigang
Hu, Min
author_facet Bao, Xingfu
Liu, Yuyan
Han, Guanghong
Zuo, Zhigang
Hu, Min
author_sort Bao, Xingfu
collection PubMed
description Cementogenesis is of great importance for normal teeth root development and is involved in the repair process of root resorption caused by orthodontic treatment. As highly differentiated mesenchymal cells, cementoblasts are responsible for this process under the regulation of many endogenous agents. Among these molecules, sclerostin has been much investigated recently for its distinct antagonism effect on bone metabolism. Encoded by the sost gene, sclerostin is expressed in osteocytes and cementocytes of cellular cementum. it is still unclear. In the current study, we investigated the effects of sclerostin on the processes of proliferation and differentiation; a series of experiments including MTT, apoptosis examination, alkaline phosphatase (ALP) activity, gene analysis, and alizarin red staining were carried out to evaluate the proliferation and differentiation of cementoblasts. Protein expression including osteoprotegerin (OPG) and receptor activator of nuclear factor kappa B ligand (RANKL) were also checked to analyze changes in osteoclastogenesis. Results show that sclerostin inhibits cementoblasts proliferation and differentiation, and promotes osteoclastogenesis. Interestingly, the monoclonal antibody for sclerostin has shown positive effects on osteoporosis, indicating that it may facilitate cementogenesis and benefit the treatment of cementum related diseases.
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spelling pubmed-38216622013-11-11 The Effect on Proliferation and Differentiation of Cementoblast by Using Sclerostin as Inhibitor Bao, Xingfu Liu, Yuyan Han, Guanghong Zuo, Zhigang Hu, Min Int J Mol Sci Article Cementogenesis is of great importance for normal teeth root development and is involved in the repair process of root resorption caused by orthodontic treatment. As highly differentiated mesenchymal cells, cementoblasts are responsible for this process under the regulation of many endogenous agents. Among these molecules, sclerostin has been much investigated recently for its distinct antagonism effect on bone metabolism. Encoded by the sost gene, sclerostin is expressed in osteocytes and cementocytes of cellular cementum. it is still unclear. In the current study, we investigated the effects of sclerostin on the processes of proliferation and differentiation; a series of experiments including MTT, apoptosis examination, alkaline phosphatase (ALP) activity, gene analysis, and alizarin red staining were carried out to evaluate the proliferation and differentiation of cementoblasts. Protein expression including osteoprotegerin (OPG) and receptor activator of nuclear factor kappa B ligand (RANKL) were also checked to analyze changes in osteoclastogenesis. Results show that sclerostin inhibits cementoblasts proliferation and differentiation, and promotes osteoclastogenesis. Interestingly, the monoclonal antibody for sclerostin has shown positive effects on osteoporosis, indicating that it may facilitate cementogenesis and benefit the treatment of cementum related diseases. Molecular Diversity Preservation International (MDPI) 2013-10-21 /pmc/articles/PMC3821662/ /pubmed/24152444 http://dx.doi.org/10.3390/ijms141021140 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Bao, Xingfu
Liu, Yuyan
Han, Guanghong
Zuo, Zhigang
Hu, Min
The Effect on Proliferation and Differentiation of Cementoblast by Using Sclerostin as Inhibitor
title The Effect on Proliferation and Differentiation of Cementoblast by Using Sclerostin as Inhibitor
title_full The Effect on Proliferation and Differentiation of Cementoblast by Using Sclerostin as Inhibitor
title_fullStr The Effect on Proliferation and Differentiation of Cementoblast by Using Sclerostin as Inhibitor
title_full_unstemmed The Effect on Proliferation and Differentiation of Cementoblast by Using Sclerostin as Inhibitor
title_short The Effect on Proliferation and Differentiation of Cementoblast by Using Sclerostin as Inhibitor
title_sort effect on proliferation and differentiation of cementoblast by using sclerostin as inhibitor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3821662/
https://www.ncbi.nlm.nih.gov/pubmed/24152444
http://dx.doi.org/10.3390/ijms141021140
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