Cargando…
Proteomic Profiling Reveals Upregulated Protein Expression of Hsp70 in Keloids
Background. The biochemical characteristics of keloid-derived fibroblasts differ from those of adjacent normal fibroblasts, and these differences are thought to be the cause of abnormal fibrosis. Therefore, we investigated the characteristic proteins that are differentially expressed in keloid-deriv...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3821890/ https://www.ncbi.nlm.nih.gov/pubmed/24260741 http://dx.doi.org/10.1155/2013/621538 |
_version_ | 1782290364244164608 |
---|---|
author | Lee, Ju Hee Shin, Jung U. Jung, Inhee Lee, Hemin Rah, Dong Kyun Jung, Jin Young Lee, Won Jai |
author_facet | Lee, Ju Hee Shin, Jung U. Jung, Inhee Lee, Hemin Rah, Dong Kyun Jung, Jin Young Lee, Won Jai |
author_sort | Lee, Ju Hee |
collection | PubMed |
description | Background. The biochemical characteristics of keloid-derived fibroblasts differ from those of adjacent normal fibroblasts, and these differences are thought to be the cause of abnormal fibrosis. Therefore, we investigated the characteristic proteins that are differentially expressed in keloid-derived fibroblasts using proteomics tools. Objective. We attempted to investigate the novel proteins that play important roles in the pathophysiology of keloids. Methods. Proteomics analysis was performed to identify differentially expressed proteins in keloid-derived fibroblasts. Keloid-derived fibroblasts and adjacent normal fibroblasts were analyzed with 2-DAGE. We validated these proteins with immunoblot analysis, real-time RT-PCR, and immunohistochemistry. Results. Sixteen differentially expressed protein spots were identified in keloid-derived fibroblasts. Among them, heat shock protein 70 (Hsp70) was specifically upregulated in keloid-derived fibroblasts. Also, immunohistochemistry and western blot analysis revealed increased Hsp70, TGF-β, and PCNA expressions in keloids compared to normal tissue. Conclusion. Hsp70 is overexpressed in keloid fibroblasts and tissue. The overexpression of Hsp70 may be involved in the pathogenesis of keloids, and the inhibition of Hsp70 could be a new therapeutic tool for the treatment of keloids. |
format | Online Article Text |
id | pubmed-3821890 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-38218902013-11-20 Proteomic Profiling Reveals Upregulated Protein Expression of Hsp70 in Keloids Lee, Ju Hee Shin, Jung U. Jung, Inhee Lee, Hemin Rah, Dong Kyun Jung, Jin Young Lee, Won Jai Biomed Res Int Research Article Background. The biochemical characteristics of keloid-derived fibroblasts differ from those of adjacent normal fibroblasts, and these differences are thought to be the cause of abnormal fibrosis. Therefore, we investigated the characteristic proteins that are differentially expressed in keloid-derived fibroblasts using proteomics tools. Objective. We attempted to investigate the novel proteins that play important roles in the pathophysiology of keloids. Methods. Proteomics analysis was performed to identify differentially expressed proteins in keloid-derived fibroblasts. Keloid-derived fibroblasts and adjacent normal fibroblasts were analyzed with 2-DAGE. We validated these proteins with immunoblot analysis, real-time RT-PCR, and immunohistochemistry. Results. Sixteen differentially expressed protein spots were identified in keloid-derived fibroblasts. Among them, heat shock protein 70 (Hsp70) was specifically upregulated in keloid-derived fibroblasts. Also, immunohistochemistry and western blot analysis revealed increased Hsp70, TGF-β, and PCNA expressions in keloids compared to normal tissue. Conclusion. Hsp70 is overexpressed in keloid fibroblasts and tissue. The overexpression of Hsp70 may be involved in the pathogenesis of keloids, and the inhibition of Hsp70 could be a new therapeutic tool for the treatment of keloids. Hindawi Publishing Corporation 2013 2013-10-24 /pmc/articles/PMC3821890/ /pubmed/24260741 http://dx.doi.org/10.1155/2013/621538 Text en Copyright © 2013 Ju Hee Lee et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Lee, Ju Hee Shin, Jung U. Jung, Inhee Lee, Hemin Rah, Dong Kyun Jung, Jin Young Lee, Won Jai Proteomic Profiling Reveals Upregulated Protein Expression of Hsp70 in Keloids |
title | Proteomic Profiling Reveals Upregulated Protein Expression of Hsp70 in Keloids |
title_full | Proteomic Profiling Reveals Upregulated Protein Expression of Hsp70 in Keloids |
title_fullStr | Proteomic Profiling Reveals Upregulated Protein Expression of Hsp70 in Keloids |
title_full_unstemmed | Proteomic Profiling Reveals Upregulated Protein Expression of Hsp70 in Keloids |
title_short | Proteomic Profiling Reveals Upregulated Protein Expression of Hsp70 in Keloids |
title_sort | proteomic profiling reveals upregulated protein expression of hsp70 in keloids |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3821890/ https://www.ncbi.nlm.nih.gov/pubmed/24260741 http://dx.doi.org/10.1155/2013/621538 |
work_keys_str_mv | AT leejuhee proteomicprofilingrevealsupregulatedproteinexpressionofhsp70inkeloids AT shinjungu proteomicprofilingrevealsupregulatedproteinexpressionofhsp70inkeloids AT junginhee proteomicprofilingrevealsupregulatedproteinexpressionofhsp70inkeloids AT leehemin proteomicprofilingrevealsupregulatedproteinexpressionofhsp70inkeloids AT rahdongkyun proteomicprofilingrevealsupregulatedproteinexpressionofhsp70inkeloids AT jungjinyoung proteomicprofilingrevealsupregulatedproteinexpressionofhsp70inkeloids AT leewonjai proteomicprofilingrevealsupregulatedproteinexpressionofhsp70inkeloids |